3EK5

Unique GTP-binding Pocket and Allostery of UMP Kinase from a Gram-Negative Phytopathogen Bacterium

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006225	biological_process	UDP biosynthetic process
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0033862	molecular_function	UMP kinase activity
A	0044210	biological_process	'de novo' CTP biosynthetic process
A	0046940	biological_process	nucleoside monophosphate phosphorylation
B	0000166	molecular_function	nucleotide binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0006225	biological_process	UDP biosynthetic process
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0033862	molecular_function	UMP kinase activity
B	0044210	biological_process	'de novo' CTP biosynthetic process
B	0046940	biological_process	nucleoside monophosphate phosphorylation
C	0000166	molecular_function	nucleotide binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006221	biological_process	pyrimidine nucleotide biosynthetic process
C	0006225	biological_process	UDP biosynthetic process
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0033862	molecular_function	UMP kinase activity
C	0044210	biological_process	'de novo' CTP biosynthetic process
C	0046940	biological_process	nucleoside monophosphate phosphorylation
D	0000166	molecular_function	nucleotide binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0006225	biological_process	UDP biosynthetic process
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0033862	molecular_function	UMP kinase activity
D	0044210	biological_process	'de novo' CTP biosynthetic process
D	0046940	biological_process	nucleoside monophosphate phosphorylation
E	0000166	molecular_function	nucleotide binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006221	biological_process	pyrimidine nucleotide biosynthetic process
E	0006225	biological_process	UDP biosynthetic process
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0033862	molecular_function	UMP kinase activity
E	0044210	biological_process	'de novo' CTP biosynthetic process
E	0046940	biological_process	nucleoside monophosphate phosphorylation
F	0000166	molecular_function	nucleotide binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006221	biological_process	pyrimidine nucleotide biosynthetic process
F	0006225	biological_process	UDP biosynthetic process
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0033862	molecular_function	UMP kinase activity
F	0044210	biological_process	'de novo' CTP biosynthetic process
F	0046940	biological_process	nucleoside monophosphate phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GTP E2001

Chain	Residue
B	ARG100
C	ARG118
C	ARG121
C	LYS125
B	ASN108
B	ASP109
B	VAL110
B	CYS111
B	GLU112
B	ARG127
C	ILE115
C	ARG117

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GTP E2002

Chain	Residue
C	ARG100
C	ASN108
C	ASP109
C	VAL110
C	CYS111
C	GLU112
C	ARG118
C	ARG127
F	ILE115
F	ARG117
F	ARG118
F	ARG121
F	LYS125

---

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GTP E2003

Chain	Residue
B	ILE115
B	ARG117
B	ARG118
B	ARG121
B	LYS125
F	ARG100
F	ASN108
F	ASP109
F	VAL110
F	CYS111
F	GLU112
F	ARG127

---

site_id	AC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE GTP E2004

Chain	Residue
A	ARG100
A	ASN108
A	ASP109
A	VAL110
A	CYS111
A	GLU112
A	ARG118
A	LYS125
A	ARG127
E	ILE115
E	ARG117
E	ARG118
E	ARG121
E	LYS125

---

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GTP E2005

Chain	Residue
A	ILE115
A	ARG117
A	ARG118
A	ARG121
A	LYS125
D	ARG100
D	ASN108
D	ASP109
D	VAL110
D	CYS111
D	GLU112
D	ARG118
D	ARG127

---

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GTP E2006

Chain	Residue
D	ILE115
D	ARG117
D	ARG118
D	ARG121
D	LYS125
E	ARG100
E	ASN108
E	ASP109
E	VAL110
E	CYS111
E	GLU112
E	ARG118
E	ARG127

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	102
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01220","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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