Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3E3K

Structural characterization of a putative endogenous metal chelator in the periplasmic nickel transporter NikA (butane-1,2,4-tricarboxylate without nickel form)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0015675	biological_process	nickel cation transport
A	0015833	biological_process	peptide transport
A	0016020	cellular_component	membrane
A	0016151	molecular_function	nickel cation binding
A	0020037	molecular_function	heme binding
A	0030288	cellular_component	outer membrane-bounded periplasmic space
A	0042597	cellular_component	periplasmic space
A	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
A	0046914	molecular_function	transition metal ion binding
A	0050919	biological_process	negative chemotaxis
A	0051540	molecular_function	metal cluster binding
A	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
A	0055085	biological_process	transmembrane transport
A	0098716	biological_process	nickel cation import across plasma membrane
A	1904680	molecular_function	peptide transmembrane transporter activity
B	0005515	molecular_function	protein binding
B	0015675	biological_process	nickel cation transport
B	0015833	biological_process	peptide transport
B	0016020	cellular_component	membrane
B	0016151	molecular_function	nickel cation binding
B	0020037	molecular_function	heme binding
B	0030288	cellular_component	outer membrane-bounded periplasmic space
B	0042597	cellular_component	periplasmic space
B	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
B	0046914	molecular_function	transition metal ion binding
B	0050919	biological_process	negative chemotaxis
B	0051540	molecular_function	metal cluster binding
B	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
B	0055085	biological_process	transmembrane transport
B	0098716	biological_process	nickel cation import across plasma membrane
B	1904680	molecular_function	peptide transmembrane transporter activity
C	0005515	molecular_function	protein binding
C	0015675	biological_process	nickel cation transport
C	0015833	biological_process	peptide transport
C	0016020	cellular_component	membrane
C	0016151	molecular_function	nickel cation binding
C	0020037	molecular_function	heme binding
C	0030288	cellular_component	outer membrane-bounded periplasmic space
C	0042597	cellular_component	periplasmic space
C	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
C	0046914	molecular_function	transition metal ion binding
C	0050919	biological_process	negative chemotaxis
C	0051540	molecular_function	metal cluster binding
C	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
C	0055085	biological_process	transmembrane transport
C	0098716	biological_process	nickel cation import across plasma membrane
C	1904680	molecular_function	peptide transmembrane transporter activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL A 503

Chain	Residue
A	LYS123

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 504

Chain	Residue
A	ASN270
A	ASP291
A	SER308
A	HIS459
A	TYR464
A	HOH527

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT A 505

Chain	Residue
A	SER57
A	GLU58

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 506

Chain	Residue
A	GLN361
A	VAL371
A	SER372
A	LEU373

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE HCT A 507

Chain	Residue
A	TYR22
A	MET27
A	TRP100
A	ARG137
A	TRP398
A	TYR402
A	HIS416

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL B 504

Chain	Residue
B	SER57
B	GLU58
B	ASP59

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT B 505

Chain	Residue
B	LYS306
B	SER308
B	HIS459
B	ALA462

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT B 506

Chain	Residue
B	ASN149
B	HOH529

site_id	AC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT B 507

Chain	Residue
B	ARG341

site_id	BC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE HCT B 508

Chain	Residue
B	TYR22
B	MET27
B	ARG97
B	TRP100
B	ARG137
B	TYR382
B	TRP398
B	TYR402
B	SER415
B	HIS416
B	HOH515

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL C 503

Chain	Residue
C	ASP331
C	ARG365

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT C 506

Chain	Residue
B	LYS433
C	ARG341

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT C 507

Chain	Residue
C	GLN385
C	HIS395
C	ARG396
C	TRP398
C	HIS416
C	HOH529

site_id	BC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE HCT C 508

Chain	Residue
C	TYR22
C	MET27
C	ARG97
C	TRP100
C	ARG137
C	ARG386
C	TRP398
C	SER415
C	HIS416

Functional Information from PROSITE/UniProt

site_id	PS01040
Number of Residues	23
Details	SBP_BACTERIAL_5 Bacterial extracellular solute-binding proteins, family 5 signature. AkswthseDgkTWtFtLRDDVKF

Chain	Residue	Details
A	ALA51-PHE73

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)