Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3E1N

Crystal structure of E. coli Bacterioferritin (BFR) after a 65 minute (aerobic) exposure to FE(II) revealing a possible MU-OXO bridge/MU-Hydroxy bridged DIIRON intermediate at the ferroxidase centre. (FE(III)-O-FE(III)-BFR).

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006826	biological_process	iron ion transport
A	0006879	biological_process	intracellular iron ion homeostasis
A	0008199	molecular_function	ferric iron binding
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0020037	molecular_function	heme binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0140315	molecular_function	iron ion sequestering activity
B	0004322	molecular_function	ferroxidase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006826	biological_process	iron ion transport
B	0006879	biological_process	intracellular iron ion homeostasis
B	0008199	molecular_function	ferric iron binding
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0020037	molecular_function	heme binding
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0140315	molecular_function	iron ion sequestering activity
C	0004322	molecular_function	ferroxidase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006826	biological_process	iron ion transport
C	0006879	biological_process	intracellular iron ion homeostasis
C	0008199	molecular_function	ferric iron binding
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0020037	molecular_function	heme binding
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0140315	molecular_function	iron ion sequestering activity
D	0004322	molecular_function	ferroxidase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006826	biological_process	iron ion transport
D	0006879	biological_process	intracellular iron ion homeostasis
D	0008199	molecular_function	ferric iron binding
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0020037	molecular_function	heme binding
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0140315	molecular_function	iron ion sequestering activity
E	0004322	molecular_function	ferroxidase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0006826	biological_process	iron ion transport
E	0006879	biological_process	intracellular iron ion homeostasis
E	0008199	molecular_function	ferric iron binding
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0020037	molecular_function	heme binding
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0140315	molecular_function	iron ion sequestering activity
F	0004322	molecular_function	ferroxidase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0006826	biological_process	iron ion transport
F	0006879	biological_process	intracellular iron ion homeostasis
F	0008199	molecular_function	ferric iron binding
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0020037	molecular_function	heme binding
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0046872	molecular_function	metal ion binding
F	0140315	molecular_function	iron ion sequestering activity
G	0004322	molecular_function	ferroxidase activity
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0006826	biological_process	iron ion transport
G	0006879	biological_process	intracellular iron ion homeostasis
G	0008199	molecular_function	ferric iron binding
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0020037	molecular_function	heme binding
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0046872	molecular_function	metal ion binding
G	0140315	molecular_function	iron ion sequestering activity
H	0004322	molecular_function	ferroxidase activity
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0006826	biological_process	iron ion transport
H	0006879	biological_process	intracellular iron ion homeostasis
H	0008199	molecular_function	ferric iron binding
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0020037	molecular_function	heme binding
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0046872	molecular_function	metal ion binding
H	0140315	molecular_function	iron ion sequestering activity
I	0004322	molecular_function	ferroxidase activity
I	0005506	molecular_function	iron ion binding
I	0005515	molecular_function	protein binding
I	0005829	cellular_component	cytosol
I	0006826	biological_process	iron ion transport
I	0006879	biological_process	intracellular iron ion homeostasis
I	0008199	molecular_function	ferric iron binding
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0020037	molecular_function	heme binding
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0046872	molecular_function	metal ion binding
I	0140315	molecular_function	iron ion sequestering activity
J	0004322	molecular_function	ferroxidase activity
J	0005506	molecular_function	iron ion binding
J	0005515	molecular_function	protein binding
J	0005829	cellular_component	cytosol
J	0006826	biological_process	iron ion transport
J	0006879	biological_process	intracellular iron ion homeostasis
J	0008199	molecular_function	ferric iron binding
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0020037	molecular_function	heme binding
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0046872	molecular_function	metal ion binding
J	0140315	molecular_function	iron ion sequestering activity
K	0004322	molecular_function	ferroxidase activity
K	0005506	molecular_function	iron ion binding
K	0005515	molecular_function	protein binding
K	0005829	cellular_component	cytosol
K	0006826	biological_process	iron ion transport
K	0006879	biological_process	intracellular iron ion homeostasis
K	0008199	molecular_function	ferric iron binding
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0020037	molecular_function	heme binding
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0046872	molecular_function	metal ion binding
K	0140315	molecular_function	iron ion sequestering activity
L	0004322	molecular_function	ferroxidase activity
L	0005506	molecular_function	iron ion binding
L	0005515	molecular_function	protein binding
L	0005829	cellular_component	cytosol
L	0006826	biological_process	iron ion transport
L	0006879	biological_process	intracellular iron ion homeostasis
L	0008199	molecular_function	ferric iron binding
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0020037	molecular_function	heme binding
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0046872	molecular_function	metal ion binding
L	0140315	molecular_function	iron ion sequestering activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 250

Chain	Residue
A	GLY145
A	LEU146
A	GLN147
A	HOH419

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 A 300

Chain	Residue
A	UNL400
A	GLU18
A	GLU51
A	HIS54
A	GLU127
A	FE2301

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 A 301

Chain	Residue
A	GLU51
A	GLU94
A	GLU127
A	HIS130
A	FE2300
A	UNL400

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 302

Chain	Residue
A	ARG117
D	ARG117
J	ARG117

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM A 200

Chain	Residue
A	ILE22
A	ASN23
A	PHE26
A	TYR45
A	MET52
A	ALA55
A	ASP56
B	LEU19
B	ASN23
B	PHE26
B	TYR45
B	MET52
B	HOH420

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 250

Chain	Residue
B	GLY145
B	LEU146
B	GLN147
B	HOH410

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 B 300

Chain	Residue
B	GLU18
B	GLU51
B	HIS54
B	GLU127
B	FE2301
B	UNL400

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 B 301

Chain	Residue
B	GLU51
B	GLU94
B	GLU127
B	HIS130
B	FE2300
B	UNL400

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 302

Chain	Residue
B	ARG117
C	ARG117
H	ARG117

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 250

Chain	Residue
C	GLY145
C	LEU146
C	GLN147
C	HOH407
C	HOH410

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 C 300

Chain	Residue
C	GLU18
C	GLU51
C	HIS54
C	GLU127
C	FE2301
C	UNL400

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 C 301

Chain	Residue
C	GLU51
C	GLU94
C	GLU127
C	HIS130
C	FE2300
C	UNL400

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 250

Chain	Residue
D	GLY145
D	LEU146
D	GLN147
D	HOH403
D	HOH404

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 D 300

Chain	Residue
D	GLU18
D	GLU51
D	HIS54
D	GLU127
D	FE2301
D	UNL400

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 D 301

Chain	Residue
D	GLU51
D	GLU94
D	GLU127
D	HIS130
D	FE2300
D	UNL400

site_id	BC7
Number of Residues	11
Details	BINDING SITE FOR RESIDUE HEM D 200

Chain	Residue
C	ASN23
C	PHE26
C	TYR45
C	MET52
C	ALA55
C	ASP56
D	ASN23
D	PHE26
D	TYR45
D	MET52
D	HOH407

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 250

Chain	Residue
E	HOH417
E	GLY145
E	LEU146
E	GLN147

site_id	BC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 E 300

Chain	Residue
E	GLU18
E	GLU51
E	HIS54
E	GLU127
E	FE2301
E	UNL400

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 E 301

Chain	Residue
E	GLU51
E	GLU94
E	GLU127
E	HIS130
E	FE2300
E	UNL400

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 F 250

Chain	Residue
F	GLY145
F	LEU146
F	GLN147
F	HOH413

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 F 300

Chain	Residue
F	GLU18
F	GLU51
F	HIS54
F	GLU127
F	FE2301
F	UNL400

site_id	CC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 F 301

Chain	Residue
F	GLU51
F	GLU94
F	GLU127
F	HIS130
F	FE2300
F	UNL400

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 F 302

Chain	Residue
F	ARG117
I	ARG117
K	ARG117

site_id	CC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HEM F 200

Chain	Residue
E	ASN23
E	PHE26
E	TYR45
E	MET52
E	ALA55
E	ASP56
F	LEU19
F	ASN23
F	PHE26
F	TYR45
F	MET52
F	HOH420

site_id	CC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 G 250

Chain	Residue
G	GLY145
G	LEU146
G	GLN147
G	HOH407

site_id	CC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 G 300

Chain	Residue
G	GLU18
G	GLU51
G	HIS54
G	GLU127
G	FE2301
G	UNL400

site_id	CC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 G 301

Chain	Residue
G	GLU51
G	GLU94
G	GLU127
G	HIS130
G	FE2300
G	UNL400

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 G 302

Chain	Residue
E	ARG117
G	ARG117
L	ARG117

site_id	DC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HEM G 200

Chain	Residue
G	ILE22
G	ASN23
G	PHE26
G	TYR45
G	MET52
G	ALA55
G	ASP56
H	ASN23
H	PHE26
H	TYR45
H	MET52
H	HOH406

site_id	DC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 H 250

Chain	Residue
H	GLY145
H	LEU146
H	GLN147

site_id	DC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 H 300

Chain	Residue
H	GLU18
H	GLU51
H	HIS54
H	GLU127
H	FE2301
H	UNL400

site_id	DC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 H 301

Chain	Residue
H	GLU51
H	GLU94
H	GLU127
H	HIS130
H	FE2300
H	UNL400

site_id	DC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 250

Chain	Residue
I	GLY145
I	LEU146
I	GLN147
I	HOH426

site_id	DC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 I 300

Chain	Residue
I	GLU18
I	GLU51
I	HIS54
I	GLU127
I	FE2301
I	UNL400

site_id	DC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 I 301

Chain	Residue
I	GLU51
I	GLU94
I	GLU127
I	HIS130
I	FE2300
I	UNL400

site_id	DC9
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM I 200

Chain	Residue
I	ILE22
I	ASN23
I	PHE26
I	TYR45
I	ILE49
I	MET52
I	ALA55
I	ASP56
J	LEU19
J	ASN23
J	PHE26
J	TYR45
J	MET52
J	HOH422

site_id	EC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 J 250

Chain	Residue
J	GLY145
J	LEU146
J	GLN147

site_id	EC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 J 300

Chain	Residue
J	GLU18
J	GLU51
J	HIS54
J	GLU127
J	FE2301
J	UNL400

site_id	EC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 J 301

Chain	Residue
J	GLU51
J	GLU94
J	GLU127
J	HIS130
J	FE2300
J	UNL400

site_id	EC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 K 250

Chain	Residue
K	GLY145
K	LEU146
K	GLN147

site_id	EC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 K 300

Chain	Residue
K	GLU18
K	GLU51
K	HIS54
K	GLU127
K	FE2301
K	UNL400

site_id	EC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 K 301

Chain	Residue
K	GLU51
K	GLU94
K	GLU127
K	HIS130
K	FE2300
K	UNL400

site_id	EC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 L 250

Chain	Residue
L	GLY145
L	LEU146
L	GLN147

site_id	EC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 L 300

Chain	Residue
L	GLU18
L	GLU51
L	HIS54
L	GLU127
L	FE2301
L	UNL400

site_id	EC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE2 L 301

Chain	Residue
L	GLU51
L	GLU94
L	GLU127
L	HIS130
L	FE2300
L	UNL400

site_id	FC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM L 200

Chain	Residue
K	ILE22
K	ASN23
K	PHE26
K	TYR45
K	MET52
K	ALA55
K	ASP56
L	LEU19
L	ASN23
L	PHE26
L	TYR45
L	ILE49
L	MET52
L	HOH407

Functional Information from PROSITE/UniProt

site_id	PS00549
Number of Residues	19
Details	BACTERIOFERRITIN Bacterioferritin signature. MkGdtkVInyLnklLgneL

Chain	Residue	Details
A	MET1-LEU19

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1728
Details	Domain: {"description":"Ferritin-like diiron","evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	96
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17077480","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"description":"axial binding residue","evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17077480","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)