Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3E1J

Crystal structure of E. coli Bacterioferritin (BFR) with an unoccupied ferroxidase centre (APO-BFR).

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006826	biological_process	iron ion transport
A	0006879	biological_process	intracellular iron ion homeostasis
A	0008199	molecular_function	ferric iron binding
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0020037	molecular_function	heme binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0140315	molecular_function	iron ion sequestering activity
B	0004322	molecular_function	ferroxidase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006826	biological_process	iron ion transport
B	0006879	biological_process	intracellular iron ion homeostasis
B	0008199	molecular_function	ferric iron binding
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0020037	molecular_function	heme binding
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0140315	molecular_function	iron ion sequestering activity
C	0004322	molecular_function	ferroxidase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006826	biological_process	iron ion transport
C	0006879	biological_process	intracellular iron ion homeostasis
C	0008199	molecular_function	ferric iron binding
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0020037	molecular_function	heme binding
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0140315	molecular_function	iron ion sequestering activity
D	0004322	molecular_function	ferroxidase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006826	biological_process	iron ion transport
D	0006879	biological_process	intracellular iron ion homeostasis
D	0008199	molecular_function	ferric iron binding
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0020037	molecular_function	heme binding
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0140315	molecular_function	iron ion sequestering activity
E	0004322	molecular_function	ferroxidase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0006826	biological_process	iron ion transport
E	0006879	biological_process	intracellular iron ion homeostasis
E	0008199	molecular_function	ferric iron binding
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0020037	molecular_function	heme binding
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0140315	molecular_function	iron ion sequestering activity
F	0004322	molecular_function	ferroxidase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0006826	biological_process	iron ion transport
F	0006879	biological_process	intracellular iron ion homeostasis
F	0008199	molecular_function	ferric iron binding
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0020037	molecular_function	heme binding
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0046872	molecular_function	metal ion binding
F	0140315	molecular_function	iron ion sequestering activity
G	0004322	molecular_function	ferroxidase activity
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0006826	biological_process	iron ion transport
G	0006879	biological_process	intracellular iron ion homeostasis
G	0008199	molecular_function	ferric iron binding
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0020037	molecular_function	heme binding
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0046872	molecular_function	metal ion binding
G	0140315	molecular_function	iron ion sequestering activity
H	0004322	molecular_function	ferroxidase activity
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0006826	biological_process	iron ion transport
H	0006879	biological_process	intracellular iron ion homeostasis
H	0008199	molecular_function	ferric iron binding
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0020037	molecular_function	heme binding
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0046872	molecular_function	metal ion binding
H	0140315	molecular_function	iron ion sequestering activity
I	0004322	molecular_function	ferroxidase activity
I	0005506	molecular_function	iron ion binding
I	0005515	molecular_function	protein binding
I	0005829	cellular_component	cytosol
I	0006826	biological_process	iron ion transport
I	0006879	biological_process	intracellular iron ion homeostasis
I	0008199	molecular_function	ferric iron binding
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0020037	molecular_function	heme binding
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0046872	molecular_function	metal ion binding
I	0140315	molecular_function	iron ion sequestering activity
J	0004322	molecular_function	ferroxidase activity
J	0005506	molecular_function	iron ion binding
J	0005515	molecular_function	protein binding
J	0005829	cellular_component	cytosol
J	0006826	biological_process	iron ion transport
J	0006879	biological_process	intracellular iron ion homeostasis
J	0008199	molecular_function	ferric iron binding
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0020037	molecular_function	heme binding
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0046872	molecular_function	metal ion binding
J	0140315	molecular_function	iron ion sequestering activity
K	0004322	molecular_function	ferroxidase activity
K	0005506	molecular_function	iron ion binding
K	0005515	molecular_function	protein binding
K	0005829	cellular_component	cytosol
K	0006826	biological_process	iron ion transport
K	0006879	biological_process	intracellular iron ion homeostasis
K	0008199	molecular_function	ferric iron binding
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0020037	molecular_function	heme binding
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0046872	molecular_function	metal ion binding
K	0140315	molecular_function	iron ion sequestering activity
L	0004322	molecular_function	ferroxidase activity
L	0005506	molecular_function	iron ion binding
L	0005515	molecular_function	protein binding
L	0005829	cellular_component	cytosol
L	0006826	biological_process	iron ion transport
L	0006879	biological_process	intracellular iron ion homeostasis
L	0008199	molecular_function	ferric iron binding
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0020037	molecular_function	heme binding
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0046872	molecular_function	metal ion binding
L	0140315	molecular_function	iron ion sequestering activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 250

Chain	Residue
A	GLY145
A	LEU146
A	GLN147
A	HOH258

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 251

Chain	Residue
A	ARG117
D	ARG117
J	ARG117

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 250

Chain	Residue
B	GLN147
B	HOH256
B	GLY145
B	LEU146

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 251

Chain	Residue
B	ARG117
C	ARG117
H	ARG117

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM B 200

Chain	Residue
A	ILE22
A	ASN23
A	PHE26
A	TYR45
A	MET52
A	ALA55
A	HOH254
B	ILE22
B	ASN23
B	PHE26
B	TYR45
B	MET52
B	HOH253
B	HOH254

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 250

Chain	Residue
C	GLY145
C	LEU146
C	GLN147
C	HOH253
C	HOH273

site_id	AC7
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM C 200

Chain	Residue
C	ILE22
C	ASN23
C	PHE26
C	TYR45
C	MET52
C	ALA55
D	LEU19
D	ASN23
D	PHE26
D	TYR45
D	MET52
D	HOH253
D	HOH265

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 250

Chain	Residue
D	GLY145
D	LEU146
D	GLN147
D	HOH251

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 250

Chain	Residue
E	GLY145
E	LEU146
E	GLN147
E	HOH251

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 F 250

Chain	Residue
F	GLY145
F	LEU146
F	GLN147
F	HOH252

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 F 251

Chain	Residue
F	ARG117
I	ARG117
I	HOH278
K	ARG117

site_id	BC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE HEM F 200

Chain	Residue
E	ILE22
E	ASN23
E	PHE26
E	TYR45
E	ILE49
E	MET52
E	ALA55
E	HOH252
F	ILE22
F	ASN23
F	PHE26
F	TYR45
F	MET52
F	HOH253
F	HOH255

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 G 250

Chain	Residue
G	GLY145
G	LEU146
G	GLN147
G	HOH252
G	HOH284

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 G 251

Chain	Residue
E	ARG117
G	ARG117
L	ARG117

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 250

Chain	Residue
H	GLY145
H	LEU146
H	GLN147
H	HOH251

site_id	BC7
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM H 200

Chain	Residue
H	TYR45
H	MET52
H	HOH252
H	HOH256
G	ILE22
G	ASN23
G	PHE26
G	TYR45
G	MET52
G	ALA55
G	HOH253
H	LEU19
H	ASN23
H	PHE26

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 250

Chain	Residue
I	GLY145
I	LEU146
I	GLN147
I	HOH251

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 J 250

Chain	Residue
J	GLY145
J	LEU146
J	GLN147
J	HOH252

site_id	CC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM J 200

Chain	Residue
I	ILE22
I	ASN23
I	PHE26
I	TYR45
I	MET52
I	ALA55
J	ILE22
J	ASN23
J	PHE26
J	TYR45
J	MET52
J	HOH253
J	HOH256

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 K 250

Chain	Residue
K	GLY145
K	LEU146
K	GLN147
K	HOH251

site_id	CC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 250

Chain	Residue
L	GLY145
L	LEU146
L	GLN147
L	HOH251

site_id	CC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM L 200

Chain	Residue
K	ILE22
K	ASN23
K	PHE26
K	TYR45
K	MET52
K	ALA55
K	HOH252
L	ILE22
L	ASN23
L	PHE26
L	TYR45
L	MET52
L	HOH253

Functional Information from PROSITE/UniProt

site_id	PS00549
Number of Residues	19
Details	BACTERIOFERRITIN Bacterioferritin signature. MkGdtkVInyLnklLgneL

Chain	Residue	Details
A	MET1-LEU19

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1728
Details	Domain: {"description":"Ferritin-like diiron","evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	96
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17077480","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"description":"axial binding residue","evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17077480","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)