3D2A
Structure of 1-17A4, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution
Functional Information from GO Data
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:11491291, ECO:0000305|PubMed:11583117, ECO:0000305|PubMed:12077437 |
Chain | Residue | Details |
A | SER77 |
site_id | SWS_FT_FI2 |
Number of Residues | 2 |
Details | ACT_SITE: Charge relay system => ECO:0000305|PubMed:11491291, ECO:0000305|PubMed:11583117, ECO:0000305|PubMed:12077437 |
Chain | Residue | Details |
A | ASP133 | |
A | HIS156 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 5 |
Details | Annotated By Reference To The Literature 1r4z |
Chain | Residue | Details |
A | ASP133 | |
A | HIS156 | |
A | MET78 | |
A | SER77 | |
A | ILE12 |
site_id | MCSA1 |
Number of Residues | 5 |
Details | M-CSA 518 |
Chain | Residue | Details |
A | ILE12 | electrostatic stabiliser |
A | SER77 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
A | MET78 | electrostatic stabiliser |
A | ASP133 | electrostatic stabiliser, increase basicity, modifies pKa |
A | HIS156 | proton acceptor, proton donor |