Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3CMC

Thioacylenzyme intermediate of Bacillus stearothermophilus phosphorylating GAPDH

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0006006	biological_process	glucose metabolic process
O	0006096	biological_process	glycolytic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0030554	molecular_function	adenyl nucleotide binding
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0006006	biological_process	glucose metabolic process
P	0006096	biological_process	glycolytic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0030554	molecular_function	adenyl nucleotide binding
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0000166	molecular_function	nucleotide binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0006006	biological_process	glucose metabolic process
Q	0006096	biological_process	glycolytic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0030554	molecular_function	adenyl nucleotide binding
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0000166	molecular_function	nucleotide binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0006006	biological_process	glucose metabolic process
R	0006096	biological_process	glycolytic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0030554	molecular_function	adenyl nucleotide binding
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 O 401

Chain	Residue
O	SER148
O	THR207
O	THR208
O	GLY209
O	ALA210

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 P 401

Chain	Residue
P	GLY209
P	ALA210
P	PRO121
P	SER148
P	THR207
P	THR208

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 Q 401

Chain	Residue
Q	PRO121
Q	SER148
Q	THR207
Q	THR208
Q	GLY209
Q	ALA210

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 R 401

Chain	Residue
R	SER148
R	THR207
R	THR208
R	GLY209
R	ALA210

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 O 402

Chain	Residue
O	THR179
O	ASP181
O	ARG195
O	ARG231

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 P 402

Chain	Residue
P	THR179
P	ASP181
P	ARG195
P	ARG231

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 Q 402

Chain	Residue
Q	THR179
Q	ASP181
Q	ARG195
Q	ARG231

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 R 402

Chain	Residue
R	THR179
R	ASP181
R	ARG195
R	ARG231

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 O 403

Chain	Residue
O	ARG169
P	LYS303

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 O 404

Chain	Residue
O	ARG183
O	HIS190
O	LYS191

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 R 403

Chain	Residue
Q	LYS303
R	ARG169

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 Q 403

Chain	Residue
Q	ARG183
Q	PRO188
Q	HIS190
Q	LYS191

site_id	BC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 R 404

Chain	Residue
R	ARG52

site_id	BC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 R 405

Chain	Residue
R	SER58

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 O 405

Chain	Residue
O	ASN62
O	ASN63

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 R 406

Chain	Residue
R	ARG183
R	HIS190
R	LYS191

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 R 407

Chain	Residue
P	ASP138
P	LYS139
R	LEU109
R	GLY112
R	ALA113
R	LYS114

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 O 406

Chain	Residue
O	GLY85
O	ALA111
O	GLY112

site_id	CC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE G3H O 400

Chain	Residue
O	SER148
O	CYS149
O	THR150
O	HIS176
O	THR179
O	THR208
O	GLY209
O	ARG231

site_id	CC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE G3H P 400

Chain	Residue
P	SER148
P	CYS149
P	THR150
P	HIS176
P	THR208
P	GLY209
P	ARG231

site_id	CC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE G3H Q 400

Chain	Residue
Q	SER148
Q	CYS149
Q	THR150
Q	HIS176
Q	THR179
Q	THR208
Q	GLY209
Q	ARG231

site_id	CC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE G3H R 400

Chain	Residue
R	SER148
R	CYS149
R	THR150
R	HIS176
R	THR179
R	THR208
R	GLY209
R	ARG231

site_id	CC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NAD O 407

Chain	Residue
O	ILE11
O	ASN31
O	ASP32
O	LEU33
O	ARG77
O	SER95
O	THR96
O	GLY97
O	ARG98
O	PHE99
O	SER119
O	ALA120
O	ASN180
O	ASN313
O	TYR317
R	LEU187
O	GLY7
O	GLY9
O	ARG10

site_id	CC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE NAD P 403

Chain	Residue
P	GLY7
P	GLY9
P	ARG10
P	ILE11
P	ASN31
P	ASP32
P	LEU33
P	ARG77
P	SER95
P	THR96
P	GLY97
P	SER119
P	ALA120
P	ASN180
P	ASN313
P	TYR317

site_id	CC7
Number of Residues	18
Details	BINDING SITE FOR RESIDUE NAD Q 404

Chain	Residue
Q	GLY7
Q	GLY9
Q	ARG10
Q	ILE11
Q	ASN31
Q	ASP32
Q	LEU33
Q	ARG77
Q	SER95
Q	THR96
Q	GLY97
Q	ARG98
Q	PHE99
Q	SER119
Q	ALA120
Q	ASN180
Q	ASN313
Q	TYR317

site_id	CC8
Number of Residues	18
Details	BINDING SITE FOR RESIDUE NAD R 408

Chain	Residue
R	GLY7
R	GLY9
R	ARG10
R	ILE11
R	ASN31
R	ASP32
R	LEU33
R	ARG77
R	SER95
R	THR96
R	GLY97
R	ARG98
R	PHE99
R	SER119
R	ALA120
R	ASN180
R	ASN313
R	TYR317

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO Q 405

Chain	Residue
Q	GLN134
Q	ASP135
Q	LYS159

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL R 409

Chain	Residue
Q	ARG169
R	LYS303

site_id	DC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL P 404

Chain	Residue
P	GLY131
P	GLN134
P	ASP135
P	LYS159

site_id	DC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL P 405

Chain	Residue
P	ASP181
P	ARG183
P	PRO188
P	HIS190
P	LYS191

site_id	DC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL O 408

Chain	Residue
O	LYS303
P	ARG169

site_id	DC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL R 410

Chain	Residue
R	GLN134
R	ASP135

site_id	DC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL P 406

Chain	Residue
P	LYS212
P	ALA215
P	GLY223

site_id	DC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL Q 406

Chain	Residue
Q	ASN14
Q	ARG17
Q	HIS50
Q	THR315

site_id	DC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL P 407

Chain	Residue
P	HIS50
P	GLY51
R	ARG281

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269\|PubMed:18480053, ECO:0000305\|PubMed:12569100

Chain	Residue	Details
O	CYS149
P	CYS149
Q	CYS149
R	CYS149

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:12569100, ECO:0000269\|PubMed:18480053, ECO:0000269\|PubMed:3586018, ECO:0000269\|PubMed:9175858

Chain	Residue	Details
O	ARG10
P	SER119
P	ASN180
P	ASN313
Q	ARG10
Q	ASP32
Q	ARG77
Q	SER119
Q	ASN180
Q	ASN313
R	ARG10
O	ASP32
R	ASP32
R	ARG77
R	SER119
R	ASN180
R	ASN313
O	ARG77
O	SER119
O	ASN180
O	ASN313
P	ARG10
P	ASP32
P	ARG77

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:12569100, ECO:0000269\|PubMed:18480053, ECO:0000269\|PubMed:3210237, ECO:0000269\|PubMed:3586018, ECO:0000269\|PubMed:9175858

Chain	Residue	Details
O	SER148
R	SER148
R	THR179
R	ARG231
O	THR179
O	ARG231
P	SER148
P	THR179
P	ARG231
Q	SER148
Q	THR179
Q	ARG231

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:12569100, ECO:0000269\|PubMed:18480053, ECO:0000269\|PubMed:9175858

Chain	Residue	Details
O	ARG195
P	ARG195
Q	ARG195
R	ARG195

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:18480053, ECO:0000269\|PubMed:3210237, ECO:0000269\|PubMed:3586018, ECO:0000269\|PubMed:9175858

Chain	Residue	Details
O	THR208
P	THR208
Q	THR208
R	THR208

site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000250\|UniProtKB:Q6GIL8

Chain	Residue	Details
O	HIS176
P	HIS176
Q	HIS176
R	HIS176

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
O	CYS149
O	HIS176

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
P	CYS149
P	HIS176

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
Q	CYS149
Q	HIS176

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
R	CYS149
R	HIS176

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)