3BYK

Crystal structure of B. subtilis levansucrase mutant D247A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009758	biological_process	carbohydrate utilization
A	0050053	molecular_function	levansucrase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 474

Chain	Residue
A	ASP241
A	GLN272
A	LEU308
A	ASN310
A	ASP339
A	HOH475

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000305|PubMed:14517548

Chain	Residue	Details
A	ASP86

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:14517548

Chain	Residue	Details
A	GLU342

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site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:3BYN

Chain	Residue	Details
A	TRP85
A	ASP86
A	SER164
A	ARG246
A	ALA247
A	GLU340
A	ARG360

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site_id	SWS_FT_FI4
Number of Residues	5
Details	BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0000269|PubMed:18596022, ECO:0000269|PubMed:32553967, ECO:0000269|PubMed:33303628, ECO:0007744|PDB:1OYG, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:2VDT, ECO:0007744|PDB:3BYJ, ECO:0007744|PDB:3BYK, ECO:0007744|PDB:3BYN, ECO:0007744|PDB:6PWQ, ECO:0007744|PDB:6VHQ

Chain	Residue	Details
A	ASP241
A	GLN272
A	LEU308
A	ASN310
A	ASP339

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site_id	SWS_FT_FI5
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:14517548

Chain	Residue	Details
A	ALA247

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1oyg

Chain	Residue	Details
A	GLU342
A	ASP86
A	ALA247

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 875

Chain	Residue	Details
A	ASP86	covalent catalysis
A	SER164	steric locator
A	ALA247	electrostatic stabiliser
A	GLU342	proton shuttle (general acid/base)

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