3BYJ
Crystal structure of B. subtilis levansucrase mutant D86A
Functional Information from GO Data
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE CA A 474 |
Chain | Residue |
A | ASP241 |
A | GLN272 |
A | LEU308 |
A | ASN310 |
A | ASP339 |
A | HOH481 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 1 |
Details | ACT_SITE: Nucleophile => ECO:0000305|PubMed:14517548 |
Chain | Residue | Details |
A | ALA86 |
site_id | SWS_FT_FI2 |
Number of Residues | 1 |
Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:14517548 |
Chain | Residue | Details |
A | GLU342 |
site_id | SWS_FT_FI3 |
Number of Residues | 7 |
Details | BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:3BYN |
Chain | Residue | Details |
A | TRP85 | |
A | ALA86 | |
A | SER164 | |
A | ARG246 | |
A | ASP247 | |
A | GLU340 | |
A | ARG360 |
site_id | SWS_FT_FI4 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:14517548, ECO:0000269|PubMed:18596022, ECO:0000269|PubMed:32553967, ECO:0000269|PubMed:33303628, ECO:0007744|PDB:1OYG, ECO:0007744|PDB:1PT2, ECO:0007744|PDB:2VDT, ECO:0007744|PDB:3BYJ, ECO:0007744|PDB:3BYK, ECO:0007744|PDB:3BYN, ECO:0007744|PDB:6PWQ, ECO:0007744|PDB:6VHQ |
Chain | Residue | Details |
A | ASP241 | |
A | GLN272 | |
A | LEU308 | |
A | ASN310 | |
A | ASP339 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:14517548 |
Chain | Residue | Details |
A | ASP247 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 3 |
Details | Annotated By Reference To The Literature 1oyg |
Chain | Residue | Details |
A | GLU342 | |
A | ALA86 | |
A | ASP247 |
site_id | MCSA1 |
Number of Residues | 4 |
Details | M-CSA 875 |
Chain | Residue | Details |
A | ALA86 | covalent catalysis |
A | SER164 | steric locator |
A | ASP247 | electrostatic stabiliser |
A | GLU342 | proton shuttle (general acid/base) |