3BUI
Golgi mannosidase II D204A catalytic nucleophile mutant complex with Tris
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000139 | cellular_component | Golgi membrane |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004559 | molecular_function | alpha-mannosidase activity |
| A | 0004572 | molecular_function | mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005795 | cellular_component | Golgi stack |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0006013 | biological_process | mannose metabolic process |
| A | 0006486 | biological_process | obsolete protein glycosylation |
| A | 0006487 | biological_process | protein N-linked glycosylation |
| A | 0006491 | biological_process | N-glycan processing |
| A | 0015923 | molecular_function | mannosidase activity |
| A | 0016063 | biological_process | rhodopsin biosynthetic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0030246 | molecular_function | carbohydrate binding |
| A | 0035010 | biological_process | encapsulation of foreign target |
| A | 0046872 | molecular_function | metal ion binding |
| A | 1904381 | biological_process | Golgi apparatus N-glycan mannose trimming |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ZN A 1046 |
| Chain | Residue |
| A | HIS90 |
| A | ASP92 |
| A | HIS471 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE TRS A 1047 |
| Chain | Residue |
| A | ASP341 |
| A | HIS471 |
| A | ASP472 |
| A | HOH2265 |
| A | HOH2282 |
| A | HOH2444 |
| A | HIS90 |
| A | ASP92 |
| A | TRP95 |
| A | ALA204 |
| A | ARG228 |
| A | TYR269 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MPD A 1048 |
| Chain | Residue |
| A | LYS63 |
| A | GLN64 |
| A | TYR267 |
| A | HIS273 |
| A | HOH1143 |
| A | HOH1597 |
| A | HOH1599 |
| A | HOH1601 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE MPD A 1049 |
| Chain | Residue |
| A | TYR435 |
| A | GLN491 |
| A | LEU494 |
| A | LYS495 |
| A | GLN498 |
| A | LEU526 |
| A | TRP531 |
| A | HOH1488 |
| A | HOH2263 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MPD A 1050 |
| Chain | Residue |
| A | ASP106 |
| A | ASP341 |
| A | PHE342 |
| A | HOH1191 |
| A | HOH1717 |
| A | HOH1718 |
| A | HOH2342 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MPD A 1051 |
| Chain | Residue |
| A | LYS495 |
| A | ALA496 |
| A | HOH1218 |
| A | HOH1494 |
| A | HOH1499 |
| A | HOH2065 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 793 |
| Chain | Residue | Details |
| A | HIS90 | metal ligand |
| A | ASP92 | metal ligand |
| A | ALA204 | covalently attached, metal ligand, nucleofuge, nucleophile |
| A | ASP341 | activator, increase nucleophilicity, proton acceptor, proton donor |
| A | HIS471 | metal ligand |
