3BSG

Barley alpha-amylase isozyme 1 (AMY1) H395A mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004556	molecular_function	alpha-amylase activity
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0005983	biological_process	starch catabolic process
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0043169	molecular_function	cation binding
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 500

Chain	Residue
A	ASN92
A	ASP139
A	ALA142
A	ASP149
A	GLY184
A	HOH630

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 501

Chain	Residue
A	ASP118
A	HOH604
A	GLU109
A	THR112
A	ASP114

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 502

Chain	Residue
A	ASP128
A	ASP143
A	PHE144
A	ALA147
A	ASP149
A	HOH788

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:7901200

Chain	Residue	Details
A	ASP180

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:7901200

Chain	Residue	Details
A	GLU205

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000305|PubMed:12906828

Chain	Residue	Details
A	HIS45
A	ARG178

---

site_id	SWS_FT_FI4
Number of Residues	7
Details	BINDING: BINDING => ECO:0000305|PubMed:16030022

Chain	Residue	Details
A	TYR52
A	TRP207
A	ASN209
A	LYS271
A	HIS290
A	GLN296
A	TRP402

---

site_id	SWS_FT_FI5
Number of Residues	13
Details	BINDING: BINDING => ECO:0000269|PubMed:12906828, ECO:0000269|PubMed:16030022, ECO:0000269|PubMed:17803687, ECO:0000269|PubMed:18588886

Chain	Residue	Details
A	ASN92
A	PHE144
A	ALA147
A	ASP149
A	GLY184
A	GLU109
A	THR112
A	ASP114
A	ASP118
A	ASP128
A	ASP139
A	ALA142
A	ASP143

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000305|PubMed:12906828, ECO:0000305|PubMed:16030022

Chain	Residue	Details
A	GLN227
A	GLY277
A	LYS375
A	THR392

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:17803687

Chain	Residue	Details
A	ASP234

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:12906828, ECO:0000305|PubMed:16030022, ECO:0000305|PubMed:17803687, ECO:0000305|PubMed:18588886

Chain	Residue	Details
A	TYR380

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:7901200

Chain	Residue	Details
A	ASP291

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP180
A	GLU205

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP234
A	ASP180

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	TRP207
A	ASP291
A	ASP180
A	GLU205

---

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	HIS290
A	ARG178
A	GLU205

---

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	HIS93
A	GLU205

---

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP291
A	ASP180
A	GLU205

---