3BP1

Crystal structure of putative 7-cyano-7-deazaguanine reductase QueF from Vibrio cholerae O1 biovar eltor

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008616	biological_process	tRNA queuosine(34) biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0033739	molecular_function	preQ1 synthase activity
A	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006400	biological_process	tRNA modification
B	0008616	biological_process	tRNA queuosine(34) biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0033739	molecular_function	preQ1 synthase activity
B	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006400	biological_process	tRNA modification
C	0008616	biological_process	tRNA queuosine(34) biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0033739	molecular_function	preQ1 synthase activity
C	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006400	biological_process	tRNA modification
D	0008616	biological_process	tRNA queuosine(34) biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0033739	molecular_function	preQ1 synthase activity
D	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 A 288

Chain	Residue
A	THR149
A	HIS186
A	HIS188
A	HOH327
A	HOH351
A	HOH448
C	PO4288

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 C 288

Chain	Residue
B	THR143
C	HIS210
C	GLN251
C	SER252
C	HIS274
A	PO4288
A	HOH351

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 D 288

Chain	Residue
B	PO4288
D	THR149
D	CYS154
D	HIS186
D	HIS188
D	HOH353
D	HOH358
D	HOH461
D	HOH483

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 B 288

Chain	Residue
B	HIS210
B	GLN251
B	SER252
B	HIS274
C	THR143
D	THR149
D	PO4288
D	HOH353

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PO4 C 289

Chain	Residue
C	THR149
C	HIS186
C	HIS188
C	HOH341
C	HOH380
C	HOH388
C	HOH546
C	HOH564

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 B 289

Chain	Residue
B	THR149
B	HIS186
B	HIS188
B	HOH350
B	HOH380
B	HOH389
B	HOH488

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 289

Chain	Residue
A	THR63
A	TYR65
A	ARG258
A	ASP266
A	ARG283
A	GLN287
A	HOH338

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG D 289

Chain	Residue
D	THR63
D	TYR65
D	ARG258
D	ASP266
D	ARG283
D	GLN287
D	HOH344

---

site_id	AC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GUN B 290

Chain	Residue
B	TRP62
B	LEU92
B	ILE93
B	GLU94
B	SER95
B	PHE232
B	HIS233
B	GLU234
B	ILE267

---

site_id	BC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GUN C 290

Chain	Residue
C	TRP62
C	LEU92
C	ILE93
C	GLU94
C	SER95
C	PHE232
C	HIS233
C	GLU234
C	ILE267
C	HOH530

---

site_id	BC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GUN A 290

Chain	Residue
A	TRP62
A	ILE93
A	GLU94
A	SER95
A	PHE232
A	HIS233
A	GLU234
A	ILE267
A	HOH469

---

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GUN D 290

Chain	Residue
D	TRP62
D	ILE93
D	GLU94
D	SER95
D	PHE232
D	HIS233
D	GLU234
D	ILE267
D	HOH487

---

site_id	BC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE POP B 291

Chain	Residue
B	LYS99
B	ARG261
B	ARG262
B	GLY263
B	HOH299
B	HOH305
B	HOH585
A	ARG262
A	GLY263

---

site_id	BC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE POP C 291

Chain	Residue
C	LYS99
C	GLN199
C	ARG261
C	ARG262
C	GLY263
C	HOH297
C	HOH300
C	HOH583
D	ARG262
D	GLY263

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Thioimide intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JAN-2013","submissionDatabase":"PDB data bank","title":"Crystal structures of 7-cyano-7-deazaguanine reductase, QueF, from Vibrio cholerae.","authoringGroup":["Center for structural genomics of infectious diseases (CSGID)"]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	Binding site: {}

Chain

Residue

Details

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