Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3BLX

Yeast Isocitrate Dehydrogenase (Apo Form)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003723	molecular_function	RNA binding
A	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006099	biological_process	tricarboxylic acid cycle
A	0006102	biological_process	isocitrate metabolic process
A	0006537	biological_process	glutamate biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0042645	cellular_component	mitochondrial nucleoid
A	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000287	molecular_function	magnesium ion binding
B	0003723	molecular_function	RNA binding
B	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006099	biological_process	tricarboxylic acid cycle
B	0006102	biological_process	isocitrate metabolic process
B	0006537	biological_process	glutamate biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
C	0000287	molecular_function	magnesium ion binding
C	0003723	molecular_function	RNA binding
C	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005758	cellular_component	mitochondrial intermembrane space
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0006099	biological_process	tricarboxylic acid cycle
C	0006102	biological_process	isocitrate metabolic process
C	0006537	biological_process	glutamate biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0042645	cellular_component	mitochondrial nucleoid
C	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
D	0000287	molecular_function	magnesium ion binding
D	0003723	molecular_function	RNA binding
D	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006099	biological_process	tricarboxylic acid cycle
D	0006102	biological_process	isocitrate metabolic process
D	0006537	biological_process	glutamate biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding
E	0000287	molecular_function	magnesium ion binding
E	0003723	molecular_function	RNA binding
E	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005758	cellular_component	mitochondrial intermembrane space
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0006099	biological_process	tricarboxylic acid cycle
E	0006102	biological_process	isocitrate metabolic process
E	0006537	biological_process	glutamate biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0042645	cellular_component	mitochondrial nucleoid
E	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
F	0000287	molecular_function	magnesium ion binding
F	0003723	molecular_function	RNA binding
F	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0006099	biological_process	tricarboxylic acid cycle
F	0006102	biological_process	isocitrate metabolic process
F	0006537	biological_process	glutamate biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
F	0046872	molecular_function	metal ion binding
F	0051287	molecular_function	NAD binding
G	0000287	molecular_function	magnesium ion binding
G	0003723	molecular_function	RNA binding
G	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005739	cellular_component	mitochondrion
G	0005758	cellular_component	mitochondrial intermembrane space
G	0005759	cellular_component	mitochondrial matrix
G	0005829	cellular_component	cytosol
G	0006099	biological_process	tricarboxylic acid cycle
G	0006102	biological_process	isocitrate metabolic process
G	0006537	biological_process	glutamate biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0042645	cellular_component	mitochondrial nucleoid
G	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
G	0046872	molecular_function	metal ion binding
G	0051287	molecular_function	NAD binding
H	0000287	molecular_function	magnesium ion binding
H	0003723	molecular_function	RNA binding
H	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005739	cellular_component	mitochondrion
H	0005759	cellular_component	mitochondrial matrix
H	0006099	biological_process	tricarboxylic acid cycle
H	0006102	biological_process	isocitrate metabolic process
H	0006537	biological_process	glutamate biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
H	0046872	molecular_function	metal ion binding
H	0051287	molecular_function	NAD binding
I	0000287	molecular_function	magnesium ion binding
I	0003723	molecular_function	RNA binding
I	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
I	0005515	molecular_function	protein binding
I	0005739	cellular_component	mitochondrion
I	0005758	cellular_component	mitochondrial intermembrane space
I	0005759	cellular_component	mitochondrial matrix
I	0005829	cellular_component	cytosol
I	0006099	biological_process	tricarboxylic acid cycle
I	0006102	biological_process	isocitrate metabolic process
I	0006537	biological_process	glutamate biosynthetic process
I	0016491	molecular_function	oxidoreductase activity
I	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
I	0042645	cellular_component	mitochondrial nucleoid
I	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
I	0046872	molecular_function	metal ion binding
I	0051287	molecular_function	NAD binding
J	0000287	molecular_function	magnesium ion binding
J	0003723	molecular_function	RNA binding
J	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
J	0005515	molecular_function	protein binding
J	0005739	cellular_component	mitochondrion
J	0005759	cellular_component	mitochondrial matrix
J	0006099	biological_process	tricarboxylic acid cycle
J	0006102	biological_process	isocitrate metabolic process
J	0006537	biological_process	glutamate biosynthetic process
J	0016491	molecular_function	oxidoreductase activity
J	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
J	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
J	0046872	molecular_function	metal ion binding
J	0051287	molecular_function	NAD binding
K	0000287	molecular_function	magnesium ion binding
K	0003723	molecular_function	RNA binding
K	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
K	0005515	molecular_function	protein binding
K	0005739	cellular_component	mitochondrion
K	0005758	cellular_component	mitochondrial intermembrane space
K	0005759	cellular_component	mitochondrial matrix
K	0005829	cellular_component	cytosol
K	0006099	biological_process	tricarboxylic acid cycle
K	0006102	biological_process	isocitrate metabolic process
K	0006537	biological_process	glutamate biosynthetic process
K	0016491	molecular_function	oxidoreductase activity
K	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
K	0042645	cellular_component	mitochondrial nucleoid
K	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
K	0046872	molecular_function	metal ion binding
K	0051287	molecular_function	NAD binding
L	0000287	molecular_function	magnesium ion binding
L	0003723	molecular_function	RNA binding
L	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
L	0005515	molecular_function	protein binding
L	0005739	cellular_component	mitochondrion
L	0005759	cellular_component	mitochondrial matrix
L	0006099	biological_process	tricarboxylic acid cycle
L	0006102	biological_process	isocitrate metabolic process
L	0006537	biological_process	glutamate biosynthetic process
L	0016491	molecular_function	oxidoreductase activity
L	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
L	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
L	0046872	molecular_function	metal ion binding
L	0051287	molecular_function	NAD binding
M	0000287	molecular_function	magnesium ion binding
M	0003723	molecular_function	RNA binding
M	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
M	0005515	molecular_function	protein binding
M	0005739	cellular_component	mitochondrion
M	0005758	cellular_component	mitochondrial intermembrane space
M	0005759	cellular_component	mitochondrial matrix
M	0005829	cellular_component	cytosol
M	0006099	biological_process	tricarboxylic acid cycle
M	0006102	biological_process	isocitrate metabolic process
M	0006537	biological_process	glutamate biosynthetic process
M	0016491	molecular_function	oxidoreductase activity
M	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
M	0042645	cellular_component	mitochondrial nucleoid
M	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
M	0046872	molecular_function	metal ion binding
M	0051287	molecular_function	NAD binding
N	0000287	molecular_function	magnesium ion binding
N	0003723	molecular_function	RNA binding
N	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
N	0005515	molecular_function	protein binding
N	0005739	cellular_component	mitochondrion
N	0005759	cellular_component	mitochondrial matrix
N	0006099	biological_process	tricarboxylic acid cycle
N	0006102	biological_process	isocitrate metabolic process
N	0006537	biological_process	glutamate biosynthetic process
N	0016491	molecular_function	oxidoreductase activity
N	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
N	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
N	0046872	molecular_function	metal ion binding
N	0051287	molecular_function	NAD binding
O	0000287	molecular_function	magnesium ion binding
O	0003723	molecular_function	RNA binding
O	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
O	0005515	molecular_function	protein binding
O	0005739	cellular_component	mitochondrion
O	0005758	cellular_component	mitochondrial intermembrane space
O	0005759	cellular_component	mitochondrial matrix
O	0005829	cellular_component	cytosol
O	0006099	biological_process	tricarboxylic acid cycle
O	0006102	biological_process	isocitrate metabolic process
O	0006537	biological_process	glutamate biosynthetic process
O	0016491	molecular_function	oxidoreductase activity
O	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
O	0042645	cellular_component	mitochondrial nucleoid
O	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
O	0046872	molecular_function	metal ion binding
O	0051287	molecular_function	NAD binding
P	0000287	molecular_function	magnesium ion binding
P	0003723	molecular_function	RNA binding
P	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
P	0005515	molecular_function	protein binding
P	0005739	cellular_component	mitochondrion
P	0005759	cellular_component	mitochondrial matrix
P	0006099	biological_process	tricarboxylic acid cycle
P	0006102	biological_process	isocitrate metabolic process
P	0006537	biological_process	glutamate biosynthetic process
P	0016491	molecular_function	oxidoreductase activity
P	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
P	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
P	0046872	molecular_function	metal ion binding
P	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	21
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NLYGDIlSDlnSglsaGSLGL

Chain	Residue	Details
B	ASN244-LEU264
A	SER237-LEU256

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
B	ARG104
F	ARG114
F	ARG135
F	ASP222
H	ARG104
H	ARG114
H	ARG135
H	ASP222
J	ARG104
J	ARG114
J	ARG135
B	ARG114
J	ASP222
L	ARG104
L	ARG114
L	ARG135
L	ASP222
N	ARG104
N	ARG114
N	ARG135
N	ASP222
P	ARG104
B	ARG135
P	ARG114
P	ARG135
P	ASP222
B	ASP222
D	ARG104
D	ARG114
D	ARG135
D	ASP222
F	ARG104

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P50213

Chain	Residue	Details
B	ASP248
J	ASP252
L	ASP248
L	ASP252
N	ASP248
N	ASP252
P	ASP248
P	ASP252
B	ASP252
D	ASP248
D	ASP252
F	ASP248
F	ASP252
H	ASP248
H	ASP252
J	ASP248

site_id	SWS_FT_FI3
Number of Residues	16
Details	SITE: Critical for catalysis => ECO:0000250

Chain	Residue	Details
B	TYR142
J	LYS189
L	TYR142
L	LYS189
N	TYR142
N	LYS189
P	TYR142
P	LYS189
B	LYS189
D	TYR142
D	LYS189
F	TYR142
F	LYS189
H	TYR142
H	LYS189
J	TYR142

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
B	THR90
D	THR90
F	THR90
H	THR90
J	THR90
L	THR90
N	THR90
P	THR90

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
B	THR138
D	THR138
F	THR138
H	THR138
J	THR138
L	THR138
N	THR138
P	THR138

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
B	THR312
D	THR312
F	THR312
H	THR312
J	THR312
L	THR312
N	THR312
P	THR312

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17761666

Chain	Residue	Details
B	THR334
D	THR334
F	THR334
H	THR334
J	THR334
L	THR334
N	THR334
P	THR334

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
B	TYR142

site_id	CSA10
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
C	PHE136

site_id	CSA11
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
E	PHE136

site_id	CSA12
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
G	PHE136

site_id	CSA13
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
I	PHE136

site_id	CSA14
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
K	PHE136

site_id	CSA15
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
M	PHE136

site_id	CSA16
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
O	PHE136

site_id	CSA17
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
B	LYS189
B	ASP222

site_id	CSA18
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
D	LYS189
D	ASP222

site_id	CSA19
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
F	LYS189
F	ASP222

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
D	TYR142

site_id	CSA20
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
H	LYS189
H	ASP222

site_id	CSA21
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
J	LYS189
J	ASP222

site_id	CSA22
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
L	LYS189
L	ASP222

site_id	CSA23
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
N	LYS189
N	ASP222

site_id	CSA24
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
P	LYS189
P	ASP222

site_id	CSA25
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
A	LYS183
A	ASP217

site_id	CSA26
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
C	LYS183
C	ASP217

site_id	CSA27
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
E	LYS183
E	ASP217

site_id	CSA28
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
G	LYS183
G	ASP217

site_id	CSA29
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
I	LYS183
I	ASP217

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
F	TYR142

site_id	CSA30
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
K	LYS183
K	ASP217

site_id	CSA31
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
M	LYS183
M	ASP217

site_id	CSA32
Number of Residues	2
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
O	LYS183
O	ASP217

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
H	TYR142

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
J	TYR142

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
L	TYR142

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
N	TYR142

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
P	TYR142

site_id	CSA9
Number of Residues	1
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
A	PHE136

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)