3B0U
tRNA-dihydrouridine synthase from Thermus thermophilus in complex with tRNA fragment
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| X | 0000049 | molecular_function | tRNA binding |
| X | 0000166 | molecular_function | nucleotide binding |
| X | 0002943 | biological_process | tRNA dihydrouridine synthesis |
| X | 0008033 | biological_process | tRNA processing |
| X | 0010181 | molecular_function | FMN binding |
| X | 0016491 | molecular_function | oxidoreductase activity |
| X | 0017150 | molecular_function | tRNA dihydrouridine synthase activity |
| X | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| X | 0102264 | molecular_function | tRNA-dihydrouridine20 synthase activity |
| X | 0102266 | molecular_function | tRNA-dihydrouridine20a synthase activity |
| Y | 0000049 | molecular_function | tRNA binding |
| Y | 0000166 | molecular_function | nucleotide binding |
| Y | 0002943 | biological_process | tRNA dihydrouridine synthesis |
| Y | 0008033 | biological_process | tRNA processing |
| Y | 0010181 | molecular_function | FMN binding |
| Y | 0016491 | molecular_function | oxidoreductase activity |
| Y | 0017150 | molecular_function | tRNA dihydrouridine synthase activity |
| Y | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| Y | 0102264 | molecular_function | tRNA-dihydrouridine20 synthase activity |
| Y | 0102266 | molecular_function | tRNA-dihydrouridine20a synthase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE FMN X 400 |
| Chain | Residue |
| A | H2U20 |
| X | HIS164 |
| X | ARG178 |
| X | ASN203 |
| X | GLY204 |
| X | GLY205 |
| X | GLY225 |
| X | ARG226 |
| X | HOH351 |
| X | HOH364 |
| X | HOH367 |
| X | ALA9 |
| X | HOH417 |
| X | PRO10 |
| X | MET11 |
| X | VAL12 |
| X | MET36 |
| X | GLN63 |
| X | ASN90 |
| X | LYS132 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE FMN Y 400 |
| Chain | Residue |
| B | H2U20 |
| Y | ALA9 |
| Y | PRO10 |
| Y | MET11 |
| Y | VAL12 |
| Y | MET36 |
| Y | GLN63 |
| Y | ASN90 |
| Y | LYS132 |
| Y | HIS164 |
| Y | ARG178 |
| Y | ASN203 |
| Y | GLY205 |
| Y | GLY225 |
| Y | ARG226 |
| Y | HOH352 |
| Y | HOH355 |
| Y | HOH402 |
| Y | HOH423 |
Functional Information from PROSITE/UniProt
| site_id | PS01136 |
| Number of Residues | 19 |
| Details | UPF0034 Uncharacterized protein family UPF0034 signature. INLNlGCPsek.Aqeggy.GA |
| Chain | Residue | Details |
| X | ILE87-ALA105 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:22123979 |
| Chain | Residue | Details |
| X | CYS93 | |
| Y | CYS93 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22123979, ECO:0007744|PDB:3B0P, ECO:0007744|PDB:3B0U, ECO:0007744|PDB:3B0V |
| Chain | Residue | Details |
| X | PRO10 | |
| Y | HIS164 | |
| Y | ASN203 | |
| Y | GLY225 | |
| X | GLN63 | |
| X | LYS132 | |
| X | HIS164 | |
| X | ASN203 | |
| X | GLY225 | |
| Y | PRO10 | |
| Y | GLN63 | |
| Y | LYS132 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Interacts with tRNA => ECO:0000269|PubMed:22123979 |
| Chain | Residue | Details |
| X | ASN90 | |
| X | ARG178 | |
| Y | ASN90 | |
| Y | ARG178 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | SITE: Interacts with tRNA; defines subfamily-specific binding signature => ECO:0000269|PubMed:22123979, ECO:0000305|PubMed:25902496 |
| Chain | Residue | Details |
| X | LYS97 | |
| X | LYS175 | |
| X | ARG290 | |
| X | ARG293 | |
| Y | LYS97 | |
| Y | LYS175 | |
| Y | ARG290 | |
| Y | ARG293 |
