3AHS

Crystal Structure of Ustilago sphaerogena Ribonuclease U2B

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0016829	molecular_function	lyase activity
A	0033899	molecular_function	ribonuclease U2 activity
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0016829	molecular_function	lyase activity
B	0033899	molecular_function	ribonuclease U2 activity
B	0046872	molecular_function	metal ion binding
C	0003723	molecular_function	RNA binding
C	0004519	molecular_function	endonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0016829	molecular_function	lyase activity
C	0033899	molecular_function	ribonuclease U2 activity
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 115

Chain	Residue
A	TYR39
C	TYR78
A	HIS41
A	GLU62
A	ARG85
A	HIS101
A	PHE110
A	HOH247
A	HOH382
A	HOH488

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K A 116

Chain	Residue
A	ASP28
A	VAL30
A	GLY33
A	HOH505

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 B 115

Chain	Residue
B	TYR39
B	HIS41
B	GLU62
B	TYR78
B	ARG85
B	HIS101
B	PHE110
B	HOH258
B	HOH282
B	HOH390

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K B 116

Chain	Residue
B	ASP28
B	VAL30
B	GLY33
B	HOH427

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 C 115

Chain	Residue
A	TYR78
C	TYR39
C	HIS41
C	GLU62
C	ARG85
C	HIS101
C	PHE110
C	HOH305
C	HOH320
C	HOH438

---

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL C 201

Chain	Residue
B	GLY10
B	THR102
B	GLY103
B	THR111
C	GLY10
C	GLY11
C	ASN12
C	THR102
C	HOH294
C	HOH329
C	HOH367
C	HOH481

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 202

Chain	Residue
A	TYR44
A	GLU46
A	HOH437

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|Ref.5

Chain	Residue	Details
A	HIS41
B	HIS41
C	HIS41

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	ACT_SITE: Proton acceptor => ECO:0000269|Ref.5

Chain	Residue	Details
A	GLU62
B	GLU62
C	GLU62

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	ACT_SITE: Proton donor => ECO:0000269|Ref.5

Chain	Residue	Details
A	HIS101
B	HIS101
C	HIS101

---

site_id	SWS_FT_FI4
Number of Residues	18
Details	BINDING: BINDING => ECO:0000269|PubMed:20858208

Chain	Residue	Details
A	ASP29
B	ASP29
B	VAL30
B	ALA31
B	IAS32
B	ASP37
B	TYR39
C	ASP29
C	VAL30
C	ALA31
C	IAS32
C	ASP37
C	TYR39
A	VAL30
A	ALA31
A	IAS32
A	ASP37
A	TYR39

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
B	ASP108
C	ARG85
C	ASP108
A	ARG85
A	ASP108
B	ARG85

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Methylation inactivates enzyme => ECO:0000269|PubMed:7492561

Chain	Residue	Details
A	GLU62
B	GLU62
C	GLU62

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
A	HIS41	proton shuttle (general acid/base)
A	GLU62	proton shuttle (general acid/base)
A	ARG85	electrostatic stabiliser
A	HIS101	proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
B	HIS41	proton shuttle (general acid/base)
B	GLU62	proton shuttle (general acid/base)
B	ARG85	electrostatic stabiliser
B	HIS101	proton shuttle (general acid/base)

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
C	HIS41	proton shuttle (general acid/base)
C	GLU62	proton shuttle (general acid/base)
C	ARG85	electrostatic stabiliser
C	HIS101	proton shuttle (general acid/base)

---