3AHS

Crystal Structure of Ustilago sphaerogena Ribonuclease U2B

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0033899	molecular_function	ribonuclease U2 activity
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0004540	molecular_function	RNA nuclease activity
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0033899	molecular_function	ribonuclease U2 activity
B	0046872	molecular_function	metal ion binding
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0004540	molecular_function	RNA nuclease activity
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0033899	molecular_function	ribonuclease U2 activity
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 115

Chain	Residue
A	TYR39
C	TYR78
A	HIS41
A	GLU62
A	ARG85
A	HIS101
A	PHE110
A	HOH247
A	HOH382
A	HOH488

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K A 116

Chain	Residue
A	ASP28
A	VAL30
A	GLY33
A	HOH505

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 B 115

Chain	Residue
B	TYR39
B	HIS41
B	GLU62
B	TYR78
B	ARG85
B	HIS101
B	PHE110
B	HOH258
B	HOH282
B	HOH390

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K B 116

Chain	Residue
B	ASP28
B	VAL30
B	GLY33
B	HOH427

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 C 115

Chain	Residue
A	TYR78
C	TYR39
C	HIS41
C	GLU62
C	ARG85
C	HIS101
C	PHE110
C	HOH305
C	HOH320
C	HOH438

---

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL C 201

Chain	Residue
B	GLY10
B	THR102
B	GLY103
B	THR111
C	GLY10
C	GLY11
C	ASN12
C	THR102
C	HOH294
C	HOH329
C	HOH367
C	HOH481

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 202

Chain	Residue
A	TYR44
A	GLU46
A	HOH437

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Active site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Active site: {"description":"Proton donor","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20858208","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	39
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Site: {"description":"Methylation inactivates enzyme","evidences":[{"source":"PubMed","id":"7492561","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
A	ASP45	proton shuttle (general acid/base)
A	VAL66	proton shuttle (general acid/base)
A	GLN89	electrostatic stabiliser
A	ALA105	proton shuttle (general acid/base)

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
B	ASP45	proton shuttle (general acid/base)
B	VAL66	proton shuttle (general acid/base)
B	GLN89	electrostatic stabiliser
B	ALA105	proton shuttle (general acid/base)

---

site_id	MCSA3
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
C	ASP45	proton shuttle (general acid/base)
C	VAL66	proton shuttle (general acid/base)
C	GLN89	electrostatic stabiliser
C	ALA105	proton shuttle (general acid/base)

---