Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3A74

Lysyl-tRNA synthetase from Bacillus stearothermophilus complexed with Diadenosine Tetraphosphate (AP4A)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004812	molecular_function	aminoacyl-tRNA ligase activity
A	0004824	molecular_function	lysine-tRNA ligase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006418	biological_process	tRNA aminoacylation for protein translation
A	0006430	biological_process	lysyl-tRNA aminoacylation
A	0016740	molecular_function	transferase activity
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
A	0140096	molecular_function	catalytic activity, acting on a protein
B	0000049	molecular_function	tRNA binding
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004812	molecular_function	aminoacyl-tRNA ligase activity
B	0004824	molecular_function	lysine-tRNA ligase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006412	biological_process	translation
B	0006418	biological_process	tRNA aminoacylation for protein translation
B	0006430	biological_process	lysyl-tRNA aminoacylation
B	0016740	molecular_function	transferase activity
B	0016874	molecular_function	ligase activity
B	0046872	molecular_function	metal ion binding
B	0140096	molecular_function	catalytic activity, acting on a protein
C	0000049	molecular_function	tRNA binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004812	molecular_function	aminoacyl-tRNA ligase activity
C	0004824	molecular_function	lysine-tRNA ligase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006412	biological_process	translation
C	0006418	biological_process	tRNA aminoacylation for protein translation
C	0006430	biological_process	lysyl-tRNA aminoacylation
C	0016740	molecular_function	transferase activity
C	0016874	molecular_function	ligase activity
C	0046872	molecular_function	metal ion binding
C	0140096	molecular_function	catalytic activity, acting on a protein
D	0000049	molecular_function	tRNA binding
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004812	molecular_function	aminoacyl-tRNA ligase activity
D	0004824	molecular_function	lysine-tRNA ligase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006412	biological_process	translation
D	0006418	biological_process	tRNA aminoacylation for protein translation
D	0006430	biological_process	lysyl-tRNA aminoacylation
D	0016740	molecular_function	transferase activity
D	0016874	molecular_function	ligase activity
D	0046872	molecular_function	metal ion binding
D	0140096	molecular_function	catalytic activity, acting on a protein

Functional Information from PDB Data

site_id	AC1
Number of Residues	30
Details	BINDING SITE FOR RESIDUE B4P A 494

Chain	Residue
A	ARG253
A	GLU366
A	GLU370
A	GLU411
A	HIS412
A	ASN414
A	GLY467
A	ARG470
A	ILE481
A	LYN495
A	HOH508
A	GLU255
A	HOH511
A	HOH543
A	HOH747
A	HOH802
A	HOH885
A	HOH914
A	MG1000
A	MG1001
A	MG1002
A	HOH1291
A	GLY256
A	HOH1300
A	SER258
A	ARG260
A	HIS261
A	ASN262
A	PHE265
A	MET267

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE LYN A 495

Chain	Residue
A	GLY207
A	ALA229
A	GLU231
A	ARG253
A	MET267
A	GLU269
A	TYR271
A	ASN414
A	PHE416
A	GLU418
A	B4P494

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1000

Chain	Residue
A	GLU404
A	GLU411
A	ALA413
A	ASN414
A	B4P494

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1001

Chain	Residue
A	B4P494
A	HOH508
A	HOH543
A	HOH802
A	HOH1291

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1002

Chain	Residue
A	GLU404
A	GLU411
A	B4P494
A	HOH1300

site_id	AC6
Number of Residues	30
Details	BINDING SITE FOR RESIDUE B4P B 494

Chain	Residue
B	ARG253
B	GLU255
B	GLY256
B	SER258
B	ARG260
B	HIS261
B	ASN262
B	PHE265
B	MET267
B	GLU366
B	GLU370
B	GLU411
B	HIS412
B	ALA413
B	ASN414
B	GLY467
B	ARG470
B	ILE481
B	LYN495
B	HOH502
B	HOH580
B	HOH864
B	HOH866
B	HOH868
B	HOH870
B	MG1100
B	MG1101
B	MG1102
B	HOH1206
C	GLN438

site_id	AC7
Number of Residues	11
Details	BINDING SITE FOR RESIDUE LYN B 495

Chain	Residue
B	GLY207
B	ALA208
B	GLU231
B	ARG253
B	MET267
B	GLU269
B	TYR271
B	ASN414
B	PHE416
B	GLU418
B	B4P494

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 1100

Chain	Residue
B	B4P494
B	HOH1206
B	GLU404
B	GLU411
B	ASN414

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 1101

Chain	Residue
B	B4P494
B	HOH580
B	HOH864
B	HOH865
B	HOH868

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 1102

Chain	Residue
B	GLU404
B	GLU411
B	B4P494
B	HOH1206

site_id	BC2
Number of Residues	31
Details	BINDING SITE FOR RESIDUE B4P C 494

Chain	Residue
C	ARG253
C	GLU255
C	GLY256
C	SER258
C	ARG260
C	HIS261
C	ASN262
C	PHE265
C	MET267
C	GLU366
C	GLU370
C	GLU411
C	HIS412
C	ASN414
C	GLY467
C	ARG470
C	ILE481
C	LYN495
C	HOH507
C	HOH509
C	HOH723
C	HOH819
C	HOH915
C	HOH917
C	HOH925
C	MG1200
C	MG1201
C	MG1202
C	HOH1302
C	HOH1303
C	HOH1304

site_id	BC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE LYN C 495

Chain	Residue
C	GLY207
C	ALA229
C	GLU231
C	ARG253
C	MET267
C	GLU269
C	TYR271
C	ASN414
C	PHE416
C	GLU418
C	B4P494

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 1200

Chain	Residue
C	GLU404
C	GLU411
C	ALA413
C	ASN414
C	B4P494

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 1201

Chain	Residue
C	B4P494
C	HOH509
C	HOH819
C	HOH915
C	HOH917

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG C 1202

Chain	Residue
C	GLU404
C	GLU411
C	B4P494
C	HOH1302

site_id	BC7
Number of Residues	29
Details	BINDING SITE FOR RESIDUE B4P D 494

Chain	Residue
A	GLN438
D	ARG253
D	GLU255
D	GLY256
D	ARG260
D	HIS261
D	ASN262
D	PHE265
D	MET267
D	GLU366
D	GLU370
D	GLU411
D	HIS412
D	ALA413
D	ASN414
D	GLY467
D	ARG470
D	ILE481
D	LYN495
D	HOH501
D	HOH596
D	HOH851
D	HOH853
D	HOH855
D	HOH1164
D	MG1300
D	MG1301
D	MG1302
D	HOH1307

site_id	BC8
Number of Residues	11
Details	BINDING SITE FOR RESIDUE LYN D 495

Chain	Residue
D	GLY207
D	ALA208
D	GLU231
D	ARG253
D	MET267
D	GLU269
D	TYR271
D	ASN414
D	PHE416
D	GLU418
D	B4P494

site_id	BC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG D 1300

Chain	Residue
D	GLU404
D	GLU411
D	ALA413
D	ASN414
D	B4P494
D	HOH1305

site_id	CC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 1301

Chain	Residue
D	B4P494
D	HOH596
D	HOH851
D	HOH852
D	HOH853

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 1302

Chain	Residue
D	GLU404
D	GLU411
D	B4P494
D	HOH1307

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)