3A2Z
E. coli Gsp amidase Cys59 sulfenic acid
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate => ECO:0000269|PubMed:9398217 |
| Chain | Residue | Details |
| A | CYS59 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | GLN58 | |
| A | ARG64 | |
| A | VAL78 | |
| A | ASN149 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Increases nucleophilicity of active site Cys; for amidase activity |
| Chain | Residue | Details |
| A | HIS131 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine sulfenic acid (-SOH); transient => ECO:0000269|PubMed:20530482 |
| Chain | Residue | Details |
| A | CYS59 |
