2ZXU
Crystal structure of tRNA modification enzyme MiaA in the complex with tRNA(Phe) and DMASPP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008033 | biological_process | tRNA processing |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| A | 0034605 | biological_process | cellular response to heat |
| A | 0052381 | molecular_function | tRNA dimethylallyltransferase activity |
| A | 1990497 | biological_process | regulation of cytoplasmic translation in response to stress |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006400 | biological_process | tRNA modification |
| B | 0008033 | biological_process | tRNA processing |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| B | 0034605 | biological_process | cellular response to heat |
| B | 0052381 | molecular_function | tRNA dimethylallyltransferase activity |
| B | 1990497 | biological_process | regulation of cytoplasmic translation in response to stress |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE DST A 317 |
| Chain | Residue |
| A | THR19 |
| A | LEU278 |
| A | MG318 |
| A | HOH343 |
| A | HOH366 |
| A | HOH372 |
| C | A37 |
| A | ALA20 |
| A | SER21 |
| A | GLY22 |
| A | LYS23 |
| A | THR24 |
| A | GLY53 |
| A | THR54 |
| A | ARG217 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE DST B 317 |
| Chain | Residue |
| B | THR19 |
| B | ALA20 |
| B | SER21 |
| B | GLY22 |
| B | LYS23 |
| B | THR24 |
| B | THR54 |
| B | ALA55 |
| B | ARG217 |
| B | MG318 |
| B | HOH322 |
| B | HOH325 |
| B | HOH348 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG C 77 |
| Chain | Residue |
| C | U16 |
| C | C17 |
| C | G19 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG C 78 |
| Chain | Residue |
| C | G27 |
| C | HOH108 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 79 |
| Chain | Residue |
| C | A7 |
| C | U8 |
| C | A14 |
| C | HOH112 |
| C | HOH113 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG D 77 |
| Chain | Residue |
| D | G27 |
| D | HOH142 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG C 80 |
| Chain | Residue |
| C | U59 |
| C | U60 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG C 81 |
| Chain | Residue |
| C | A58 |
| C | U59 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG C 82 |
| Chain | Residue |
| C | G22 |
| C | U45 |
| C | G46 |
| C | HOH195 |
| site_id | BC1 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE MG C 83 |
| Chain | Residue |
| C | U12 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG A 318 |
| Chain | Residue |
| A | THR24 |
| A | DST317 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG B 318 |
| Chain | Residue |
| B | THR24 |
| B | ALA55 |
| B | DST317 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 46 |
| Details | Region: {"description":"Interaction with substrate tRNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 46 |
| Details | Region: {"description":"Interaction with isopentenylpyrophosphate transferase"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Site: {"description":"Interaction with substrate tRNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Required for specificity towards tRNA substrates containing a purine at position 29"} |
| Chain | Residue | Details |
