2ZO5
Structure of the Thioalkalivibrio nitratireducens cytochrome c nitrite reductase in a complex with azide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0019331 | biological_process | anaerobic respiration, using ammonium as electron donor |
| A | 0019645 | biological_process | anaerobic electron transport chain |
| A | 0020037 | molecular_function | heme binding |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0042128 | biological_process | nitrate assimilation |
| A | 0042279 | molecular_function | nitrite reductase (cytochrome, ammonia-forming) activity |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0019331 | biological_process | anaerobic respiration, using ammonium as electron donor |
| B | 0019645 | biological_process | anaerobic electron transport chain |
| B | 0020037 | molecular_function | heme binding |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0042128 | biological_process | nitrate assimilation |
| B | 0042279 | molecular_function | nitrite reductase (cytochrome, ammonia-forming) activity |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE HEC A 1004 |
| Chain | Residue |
| A | HIS113 |
| A | ARG242 |
| A | CYS299 |
| A | HIS300 |
| A | TYR303 |
| A | CYS305 |
| A | HIS361 |
| A | ASN486 |
| A | AZI526 |
| A | HEC1006 |
| A | HOH1014 |
| A | ALA114 |
| A | HOH1029 |
| A | HOH1149 |
| A | HOH1150 |
| A | HOH1156 |
| A | HOH1165 |
| A | HOH1377 |
| A | ASP125 |
| A | HIS126 |
| A | VAL129 |
| A | ARG131 |
| A | CYS184 |
| A | CYS187 |
| A | LYS188 |
| site_id | AC2 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE HEC A 1005 |
| Chain | Residue |
| A | CYS66 |
| A | HIS70 |
| A | GLN73 |
| A | LEU225 |
| A | CYS227 |
| A | CYS230 |
| A | HIS231 |
| A | ALA290 |
| A | MET384 |
| A | TYR395 |
| A | THR396 |
| A | HIS398 |
| A | HEC1003 |
| A | HOH1122 |
| A | HOH1234 |
| A | HOH1255 |
| A | HOH1289 |
| A | HOH1535 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE HEC A 1006 |
| Chain | Residue |
| A | SER84 |
| A | PRO116 |
| A | ARG117 |
| A | HIS119 |
| A | PHE121 |
| A | MET122 |
| A | ASP125 |
| A | CYS187 |
| A | LYS188 |
| A | MET229 |
| A | CYS296 |
| A | CYS299 |
| A | HIS300 |
| A | HIS383 |
| A | MET384 |
| A | CA528 |
| A | HEC1004 |
| A | HEC1007 |
| A | HOH1018 |
| A | HOH1036 |
| A | HOH1144 |
| site_id | AC4 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HEC A 1007 |
| Chain | Residue |
| A | HIS300 |
| A | PHE367 |
| A | HIS372 |
| A | ALA378 |
| A | CYS379 |
| A | CYS382 |
| A | HIS383 |
| A | THR402 |
| A | PRO403 |
| A | ARG404 |
| A | ILE427 |
| A | LYS431 |
| A | ASN486 |
| A | PHE490 |
| A | HIS491 |
| A | CA528 |
| A | HEC1006 |
| A | HOH1144 |
| A | HOH1145 |
| A | HOH1153 |
| A | HOH1157 |
| A | HOH1336 |
| A | HOH1364 |
| site_id | AC5 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE HEC A 1008 |
| Chain | Residue |
| A | PHE490 |
| A | HOH1423 |
| A | HOH1461 |
| A | HOH1463 |
| A | HOH1529 |
| A | ASN141 |
| A | TRP142 |
| A | GLN143 |
| A | VAL371 |
| A | HIS372 |
| A | PRO403 |
| A | ALA410 |
| A | CYS411 |
| A | CYS414 |
| A | HIS415 |
| A | TRP418 |
| A | ILE427 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE HEC A 1002 |
| Chain | Residue |
| A | CYS14 |
| A | PHE15 |
| A | HIS18 |
| A | VAL33 |
| A | ASN34 |
| A | CYS35 |
| A | CYS38 |
| A | HIS39 |
| A | THR59 |
| A | LEU194 |
| A | PHE228 |
| A | PRO233 |
| A | HIS234 |
| A | ARG239 |
| A | PHE274 |
| A | ARG276 |
| A | ARG282 |
| A | HEC1001 |
| A | HEC1003 |
| A | HOH1089 |
| A | HOH1346 |
| A | HOH1475 |
| site_id | AC7 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE HEC A 1003 |
| Chain | Residue |
| A | LYS29 |
| A | HIS30 |
| A | HIS37 |
| A | ALA65 |
| A | CYS66 |
| A | THR68 |
| A | CYS69 |
| A | HIS70 |
| A | CYS227 |
| A | HIS231 |
| A | ALA236 |
| A | HEC1002 |
| A | HEC1005 |
| A | HOH1120 |
| A | HOH1314 |
| A | HOH1359 |
| A | HOH1398 |
| A | HOH1439 |
| A | HOH1550 |
| B | THR68 |
| site_id | AC8 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE HEC A 1001 |
| Chain | Residue |
| A | GLN13 |
| A | CYS14 |
| A | CYS17 |
| A | HIS18 |
| A | HIS39 |
| A | HIS44 |
| A | VAL45 |
| A | ALA48 |
| A | SER49 |
| A | SER50 |
| A | ARG52 |
| A | MET53 |
| A | ARG56 |
| A | PRO57 |
| A | THR59 |
| A | LEU194 |
| A | ARG276 |
| A | GLY277 |
| A | HEC1002 |
| A | HOH1092 |
| A | HOH1119 |
| A | HOH1474 |
| A | HOH1476 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE AZI A 526 |
| Chain | Residue |
| A | ARG131 |
| A | TYR303 |
| A | GLN360 |
| A | HIS361 |
| A | HEC1004 |
| A | HOH1014 |
| A | HOH1369 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 527 |
| Chain | Residue |
| A | GLU302 |
| A | TYR303 |
| A | LYS358 |
| A | GLN360 |
| A | HOH1237 |
| A | HOH1238 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 528 |
| Chain | Residue |
| A | PRO116 |
| A | HEC1006 |
| A | HEC1007 |
| A | HOH1049 |
| A | HOH1336 |
| A | HOH1396 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE NA A 529 |
| Chain | Residue |
| A | ASP450 |
| A | HOH1415 |
| A | HOH1415 |
| A | HOH1415 |
| A | HOH1416 |
| A | HOH1416 |
| A | HOH1416 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 A 1009 |
| Chain | Residue |
| A | THR94 |
| A | SER95 |
| A | LYS431 |
| A | HIS435 |
| A | HOH1288 |
| A | HOH1544 |
| site_id | BC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG6 A 1010 |
| Chain | Residue |
| A | TYR309 |
| A | LEU311 |
| A | GLY314 |
| A | ARG348 |
| A | ALA355 |
| A | HOH1252 |
| A | HOH1307 |
| site_id | BC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 A 1011 |
| Chain | Residue |
| A | GLN138 |
| A | PHE139 |
| A | ASN141 |
| A | TRP142 |
| A | ASP494 |
| A | HOH1362 |
| site_id | BC7 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE TRS A 1012 |
| Chain | Residue |
| A | MET99 |
| A | MET99 |
| A | LYS102 |
| A | LYS102 |
| A | LYS102 |
| A | AZI530 |
| A | AZI530 |
| A | HOH1052 |
| A | HOH1052 |
| A | HOH1328 |
| A | HOH1328 |
| A | HOH1328 |
| A | HOH1415 |
| A | HOH1415 |
| A | HOH1415 |
| site_id | BC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE AZI A 530 |
| Chain | Residue |
| A | PRO98 |
| A | TRS1012 |
| A | TRS1012 |
| A | HOH1046 |
| A | HOH1290 |
| A | HOH1394 |
| site_id | BC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 A 1013 |
| Chain | Residue |
| A | MET12 |
| A | ALA31 |
| A | THR32 |
| A | ASN34 |
| A | HOH1335 |
| B | PRO8 |
| B | VAL9 |
| B | PG41009 |
| site_id | CC1 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE HEC B 1004 |
| Chain | Residue |
| B | HIS113 |
| B | ALA114 |
| B | ASP125 |
| B | HIS126 |
| B | VAL129 |
| B | ARG131 |
| B | ALA179 |
| B | CYS184 |
| B | CYS187 |
| B | LYS188 |
| B | ARG242 |
| B | CYS299 |
| B | HIS300 |
| B | VAL301 |
| B | TYR303 |
| B | CYS305 |
| B | HIS361 |
| B | ASN486 |
| B | AZI526 |
| B | HEC1006 |
| B | HOH1039 |
| B | HOH1090 |
| B | HOH1210 |
| B | HOH1211 |
| B | HOH1217 |
| B | HOH1437 |
| site_id | CC2 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE HEC B 1005 |
| Chain | Residue |
| B | CYS66 |
| B | HIS70 |
| B | GLN73 |
| B | PHE74 |
| B | LEU225 |
| B | ASN226 |
| B | CYS227 |
| B | CYS230 |
| B | HIS231 |
| B | ALA290 |
| B | MET384 |
| B | TYR395 |
| B | THR396 |
| B | HIS398 |
| B | HEC1003 |
| B | HEC1006 |
| B | HOH1183 |
| B | HOH1295 |
| B | HOH1316 |
| B | HOH1350 |
| B | HOH1595 |
| site_id | CC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE HEC B 1006 |
| Chain | Residue |
| B | SER84 |
| B | PRO116 |
| B | ARG117 |
| B | HIS119 |
| B | PHE121 |
| B | MET122 |
| B | ASP125 |
| B | CYS187 |
| B | MET229 |
| B | CYS296 |
| B | CYS299 |
| B | HIS300 |
| B | HIS383 |
| B | MET384 |
| B | CA528 |
| B | HEC1004 |
| B | HEC1005 |
| B | HEC1007 |
| B | HOH1079 |
| B | HOH1097 |
| B | HOH1205 |
| site_id | CC4 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE HEC B 1007 |
| Chain | Residue |
| B | HIS300 |
| B | PHE367 |
| B | HIS372 |
| B | ALA378 |
| B | CYS379 |
| B | CYS382 |
| B | HIS383 |
| B | THR402 |
| B | PRO403 |
| B | ARG404 |
| B | LYS431 |
| B | ASN486 |
| B | PHE490 |
| B | HIS491 |
| B | CA528 |
| B | HEC1006 |
| B | HOH1205 |
| B | HOH1206 |
| B | HOH1214 |
| B | HOH1218 |
| B | HOH1397 |
| B | HOH1424 |
| site_id | CC5 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE HEC B 1008 |
| Chain | Residue |
| B | ASN141 |
| B | TRP142 |
| B | GLN143 |
| B | VAL371 |
| B | HIS372 |
| B | PRO403 |
| B | ALA410 |
| B | CYS411 |
| B | CYS414 |
| B | HIS415 |
| B | TRP418 |
| B | ILE427 |
| B | PHE490 |
| B | PG41016 |
| B | HOH1483 |
| B | HOH1530 |
| B | HOH1556 |
| site_id | CC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE HEC B 1002 |
| Chain | Residue |
| B | CYS14 |
| B | PHE15 |
| B | HIS18 |
| B | ILE21 |
| B | HIS25 |
| B | VAL33 |
| B | ASN34 |
| B | CYS35 |
| B | CYS38 |
| B | HIS39 |
| B | LEU194 |
| B | PHE228 |
| B | PRO233 |
| B | HIS234 |
| B | ARG239 |
| B | PHE274 |
| B | ARG276 |
| B | ARG282 |
| B | HEC1001 |
| B | HEC1003 |
| B | HOH1150 |
| B | HOH1407 |
| site_id | CC7 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE HEC B 1003 |
| Chain | Residue |
| A | THR68 |
| B | LYS29 |
| B | HIS30 |
| B | HIS37 |
| B | ALA65 |
| B | CYS66 |
| B | CYS69 |
| B | HIS70 |
| B | CYS227 |
| B | HIS231 |
| B | ALA236 |
| B | HEC1002 |
| B | HEC1005 |
| B | HOH1180 |
| B | HOH1375 |
| B | HOH1419 |
| B | HOH1458 |
| B | HOH1594 |
| site_id | CC8 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE HEC B 1001 |
| Chain | Residue |
| B | GLN13 |
| B | CYS14 |
| B | CYS17 |
| B | HIS18 |
| B | HIS39 |
| B | ALA41 |
| B | HIS44 |
| B | VAL45 |
| B | ALA48 |
| B | SER49 |
| B | SER50 |
| B | ARG51 |
| B | ARG51 |
| B | ARG52 |
| B | MET53 |
| B | ARG56 |
| B | PRO57 |
| B | THR59 |
| B | LEU194 |
| B | GLN275 |
| B | ARG276 |
| B | HEC1002 |
| B | HOH1153 |
| B | HOH1179 |
| B | HOH1407 |
| B | HOH1517 |
| B | HOH1522 |
| B | HOH1538 |
| site_id | CC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1009 |
| Chain | Residue |
| A | THR32 |
| A | PG41013 |
| A | HOH1534 |
| B | ALA31 |
| B | THR32 |
| B | ASN34 |
| B | HOH1396 |
| site_id | DC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE AZI B 526 |
| Chain | Residue |
| B | ARG131 |
| B | TYR303 |
| B | GLN360 |
| B | HIS361 |
| B | HEC1004 |
| B | HOH1039 |
| B | HOH1429 |
| B | HOH1437 |
| site_id | DC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B 527 |
| Chain | Residue |
| B | GLU302 |
| B | TYR303 |
| B | LYS358 |
| B | GLN360 |
| B | HOH1298 |
| B | HOH1299 |
| site_id | DC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CA B 528 |
| Chain | Residue |
| B | PRO116 |
| B | HEC1006 |
| B | HEC1007 |
| B | PG41014 |
| B | HOH1110 |
| B | HOH1397 |
| B | HOH1456 |
| site_id | DC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA B 529 |
| Chain | Residue |
| B | ASP450 |
| B | HOH1475 |
| B | HOH1475 |
| B | HOH1475 |
| B | HOH1476 |
| B | HOH1476 |
| site_id | DC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1010 |
| Chain | Residue |
| B | THR94 |
| B | LYS431 |
| B | HIS435 |
| B | HOH1478 |
| B | HOH1535 |
| B | HOH1607 |
| site_id | DC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG6 B 1011 |
| Chain | Residue |
| B | TYR309 |
| B | LEU311 |
| B | GLY314 |
| B | ARG348 |
| B | ALA355 |
| B | HOH1313 |
| B | HOH1368 |
| site_id | DC7 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE TRS B 1012 |
| Chain | Residue |
| B | MET99 |
| B | MET99 |
| B | LYS102 |
| B | LYS102 |
| B | AZI530 |
| B | AZI530 |
| B | HOH1113 |
| B | HOH1113 |
| B | HOH1389 |
| B | HOH1389 |
| B | HOH1389 |
| B | HOH1475 |
| B | HOH1475 |
| B | HOH1612 |
| B | HOH1612 |
| site_id | DC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1013 |
| Chain | Residue |
| B | PHE172 |
| B | SER174 |
| site_id | DC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE AZI B 530 |
| Chain | Residue |
| B | PRO98 |
| B | TRS1012 |
| B | TRS1012 |
| B | HOH1107 |
| B | HOH1351 |
| B | HOH1454 |
| B | HOH1454 |
| site_id | EC1 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1014 |
| Chain | Residue |
| B | ARG81 |
| B | GLU83 |
| B | SER84 |
| B | HIS85 |
| B | GLU115 |
| B | PRO116 |
| B | CA528 |
| B | HOH1206 |
| B | HOH1367 |
| B | HOH1379 |
| B | HOH1393 |
| B | HOH1397 |
| B | HOH1424 |
| B | HOH1446 |
| B | HOH1456 |
| B | HOH1548 |
| B | HOH1582 |
| site_id | EC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1015 |
| Chain | Residue |
| B | LEU311 |
| B | LYS336 |
| B | GLN340 |
| B | GLU470 |
| B | TYR473 |
| B | HOH1325 |
| B | HOH1581 |
| site_id | EC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1016 |
| Chain | Residue |
| B | SER370 |
| B | VAL371 |
| B | ASN492 |
| B | PRO493 |
| B | ASP494 |
| B | HEC1008 |
| B | HOH1104 |
| B | HOH1566 |
| site_id | EC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 B 1017 |
| Chain | Residue |
| B | ARG60 |
| B | ASP62 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 32 |
| Details | Binding site: {"description":"covalent","evidences":[{"source":"PubMed","id":"19393666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20944237","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22281743","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4L38","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3Y","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 28 |
| Details | Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"19393666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20944237","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22281743","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4L38","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3Y","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"19393666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20944237","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22281743","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"19393666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20944237","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22281743","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4L38","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4L3Y","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"3'-(S-cysteinyl)-tyrosine (Tyr-Cys)","evidences":[{"source":"PubMed","id":"19393666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22281743","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
