2Z3H
Crystal structure of blasticidin S deaminase (BSD) complexed with deaminohydroxy blasticidin S
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004126 | molecular_function | cytidine deaminase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0047711 | molecular_function | blasticidin-S deaminase activity |
| A | 0055086 | biological_process | nucleobase-containing small molecule metabolic process |
| A | 0072527 | biological_process | pyrimidine-containing compound metabolic process |
| A | 1901135 | biological_process | carbohydrate derivative metabolic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004126 | molecular_function | cytidine deaminase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046677 | biological_process | response to antibiotic |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0047711 | molecular_function | blasticidin-S deaminase activity |
| B | 0055086 | biological_process | nucleobase-containing small molecule metabolic process |
| B | 0072527 | biological_process | pyrimidine-containing compound metabolic process |
| B | 1901135 | biological_process | carbohydrate derivative metabolic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004126 | molecular_function | cytidine deaminase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008270 | molecular_function | zinc ion binding |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046677 | biological_process | response to antibiotic |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0047711 | molecular_function | blasticidin-S deaminase activity |
| C | 0055086 | biological_process | nucleobase-containing small molecule metabolic process |
| C | 0072527 | biological_process | pyrimidine-containing compound metabolic process |
| C | 1901135 | biological_process | carbohydrate derivative metabolic process |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004126 | molecular_function | cytidine deaminase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046677 | biological_process | response to antibiotic |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0047711 | molecular_function | blasticidin-S deaminase activity |
| D | 0055086 | biological_process | nucleobase-containing small molecule metabolic process |
| D | 0072527 | biological_process | pyrimidine-containing compound metabolic process |
| D | 1901135 | biological_process | carbohydrate derivative metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 2001 |
| Chain | Residue |
| A | CYS54 |
| A | CYS88 |
| A | CYS91 |
| A | BLO2500 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 2002 |
| Chain | Residue |
| B | CYS54 |
| B | CYS88 |
| B | CYS91 |
| B | BLO2501 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C 2003 |
| Chain | Residue |
| C | CYS88 |
| C | CYS91 |
| C | BLO2502 |
| C | CYS54 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ZN D 2004 |
| Chain | Residue |
| D | CYS54 |
| D | CYS88 |
| D | CYS91 |
| D | BLO2503 |
| D | HOH2617 |
| site_id | AC5 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE BLO A 2500 |
| Chain | Residue |
| A | GLU25 |
| A | ASP26 |
| A | SER28 |
| A | VAL29 |
| A | ASN45 |
| A | TYR47 |
| A | CYS54 |
| A | ALA55 |
| A | GLU56 |
| A | ARG82 |
| A | PRO87 |
| A | CYS88 |
| A | CYS91 |
| A | ZN2001 |
| A | HOH2510 |
| A | HOH2623 |
| A | HOH2629 |
| A | HOH2631 |
| B | TYR126 |
| B | TRP128 |
| C | PHE49 |
| site_id | AC6 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE BLO B 2501 |
| Chain | Residue |
| B | GLU25 |
| B | ASP26 |
| B | SER28 |
| B | VAL29 |
| B | ASN45 |
| B | TYR47 |
| B | CYS54 |
| B | ALA55 |
| B | GLU56 |
| B | ARG82 |
| B | PRO87 |
| B | CYS88 |
| B | ZN2002 |
| B | HOH2504 |
| B | HOH2550 |
| B | HOH2574 |
| B | HOH2647 |
| B | HOH2649 |
| B | HOH2679 |
| D | PHE49 |
| site_id | AC7 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE BLO C 2502 |
| Chain | Residue |
| A | PHE49 |
| C | GLU25 |
| C | ASP26 |
| C | SER28 |
| C | VAL29 |
| C | ASN45 |
| C | TYR47 |
| C | CYS54 |
| C | ALA55 |
| C | GLU56 |
| C | ARG82 |
| C | PRO87 |
| C | CYS88 |
| C | CYS91 |
| C | ZN2003 |
| C | HOH2505 |
| C | HOH2537 |
| C | HOH2572 |
| C | HOH2585 |
| C | HOH2612 |
| C | HOH2629 |
| site_id | AC8 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE BLO D 2503 |
| Chain | Residue |
| B | PHE49 |
| D | GLU25 |
| D | ASP26 |
| D | SER28 |
| D | VAL29 |
| D | ASN45 |
| D | TYR47 |
| D | CYS54 |
| D | ALA55 |
| D | GLU56 |
| D | ARG82 |
| D | PRO87 |
| D | CYS88 |
| D | CYS91 |
| D | ZN2004 |
| D | HOH2504 |
| D | HOH2537 |
| D | HOH2625 |
| D | HOH2628 |
| D | HOH2629 |
Functional Information from PROSITE/UniProt
| site_id | PS00903 |
| Number of Residues | 42 |
| Details | CYT_DCMP_DEAMINASES_1 Cytidine and deoxycytidylate deaminases zinc-binding region signature. CAElvVLgtaaaaaagnltcivaignenrgils.......PCgr......CrqvL |
| Chain | Residue | Details |
| A | CYS54-LEU95 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | GLU56 | |
| B | GLU56 | |
| C | GLU56 | |
| D | GLU56 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: in other chain |
| Chain | Residue | Details |
| A | SER28 | |
| D | SER28 | |
| D | ARG82 | |
| D | TRP128 | |
| A | ARG82 | |
| A | TRP128 | |
| B | SER28 | |
| B | ARG82 | |
| B | TRP128 | |
| C | SER28 | |
| C | ARG82 | |
| C | TRP128 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | CYS54 | |
| C | CYS88 | |
| C | CYS91 | |
| C | TYR126 | |
| D | CYS54 | |
| D | CYS88 | |
| D | CYS91 | |
| D | TYR126 | |
| A | CYS88 | |
| A | CYS91 | |
| A | TYR126 | |
| B | CYS54 | |
| B | CYS88 | |
| B | CYS91 | |
| B | TYR126 | |
| C | CYS54 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ctt |
| Chain | Residue | Details |
| A | GLU56 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ctt |
| Chain | Residue | Details |
| B | GLU56 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ctt |
| Chain | Residue | Details |
| C | GLU56 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ctt |
| Chain | Residue | Details |
| D | GLU56 |
