2YZN

Crystal structure of D-alanine:D-Alanine Ligase with AMPPNP from Thermus thermophilus HB8.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008360	biological_process	regulation of cell shape
A	0008716	molecular_function	D-alanine-D-alanine ligase activity
A	0009252	biological_process	peptidoglycan biosynthetic process
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
A	0071555	biological_process	cell wall organization
B	0000166	molecular_function	nucleotide binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008360	biological_process	regulation of cell shape
B	0008716	molecular_function	D-alanine-D-alanine ligase activity
B	0009252	biological_process	peptidoglycan biosynthetic process
B	0016874	molecular_function	ligase activity
B	0046872	molecular_function	metal ion binding
B	0071555	biological_process	cell wall organization
C	0000166	molecular_function	nucleotide binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008360	biological_process	regulation of cell shape
C	0008716	molecular_function	D-alanine-D-alanine ligase activity
C	0009252	biological_process	peptidoglycan biosynthetic process
C	0016874	molecular_function	ligase activity
C	0046872	molecular_function	metal ion binding
C	0071555	biological_process	cell wall organization

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ANP A 401

Chain	Residue
A	LYS116
A	ASN281
A	GLU282
A	PHE151
A	LYS153
A	SER160
A	ILE163
A	GLU189
A	LYS190
A	LEU192
A	GLU197

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ANP B 402

Chain	Residue
B	LYS116
B	VAL131
B	PHE151
B	LYS153
B	THR157
B	GLY158
B	SER159
B	SER160
B	ILE163
B	GLU189
B	LYS190
B	ALA191
B	LEU192
B	GLU197
B	PHE272
B	ASN281
B	GLU282
C	ALA220

---

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ANP C 403

Chain	Residue
B	ALA220
C	LYS116
C	PHE151
C	LYS153
C	SER159
C	SER160
C	LYS190
C	ALA191
C	LEU192
C	GLU197
C	ARG268
C	PHE272
C	ASN281
C	GLU282
C	ASN284

---

Functional Information from PROSITE/UniProt

site_id	PS00843
Number of Residues	12
Details	DALA_DALA_LIGASE_1 D-alanine--D-alanine ligase signature 1. HGrfGEDGtVQG

Chain	Residue	Details
A	HIS82-GLY93

---

site_id	PS00844
Number of Residues	28
Details	DALA_DALA_LIGASE_2 D-alanine--D-alanine ligase signature 2. LgvrGmARVDFFlaegely.....LnELNTiPG

Chain	Residue	Details
A	LEU261-GLY288

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	111
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00047","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 2dln

Chain	Residue	Details
A	GLU13
A	ARG268
A	TYR229
A	GLY288

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 2dln

Chain	Residue	Details
B	GLU13
B	ARG268
B	GLY288
B	SER159

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 2dln

Chain	Residue	Details
C	GLU13
C	ARG268
C	GLY288
C	SER159

---