2Y5B

Structure of USP21 in complex with linear diubiquitin-aldehyde

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0016579	biological_process	protein deubiquitination
E	0004843	molecular_function	cysteine-type deubiquitinase activity
E	0016579	biological_process	protein deubiquitination

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 1000

Chain	Residue
A	CYS384
A	CYS387
A	CYS437
A	CYS440

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 1559

Chain	Residue
A	SER472
A	ILE473

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 1560

Chain	Residue
A	VAL514
F	ARG54
A	LYS474
A	LYS475
A	SER513

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 E 1560

Chain	Residue
E	PRO235
E	LYS369
E	ARG457

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 E 1561

Chain	Residue
E	GLU276
E	ALA277

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 1561

Chain	Residue
A	ARG320

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 1562

Chain	Residue
A	PRO235
A	LYS369
A	ARG457

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 1563

Chain	Residue
A	PRO483
A	LEU484
A	GLN485

---

site_id	AC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 E 1562

Chain	Residue
E	ARG320

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 1564

Chain	Residue
A	SER541
A	ASN543
E	GLY296

---

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 1152

Chain	Residue
A	HIS515
B	GLN116
B	ARG150

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 F 1152

Chain	Residue
F	GLN116
F	ARG148
F	ARG150

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 1565

Chain	Residue
A	ARG215
A	ASN216
A	ARG390
A	ASP534
A	SER535

---

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 1000

Chain	Residue
E	CYS384
E	CYS387
E	CYS437
E	CYS440

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52
B	LYS103-ASP128

---

site_id	PS00972
Number of Residues	16
Details	USP_1 Ubiquitin specific protease (USP) domain signature 1. GLrnlGNtCFLNAvLQ

Chain	Residue	Details
A	GLY213-GLN228

---

site_id	PS00973
Number of Residues	18
Details	USP_2 Ubiquitin specific protease (USP) domain signature 2. YqLyALcnHsGsvhy..GHY

Chain	Residue	Details
A	TYR502-TYR519

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"10799498","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"32011234","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10092","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10093","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21399617","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2Y5B","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	75
Details	Domain: {"description":"Ubiquitin-like 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---