2XXF

Cu metallated H254F mutant of nitrite reductase

Replaces:  2JL3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005507	molecular_function	copper ion binding
A	0005515	molecular_function	protein binding
A	0006807	biological_process	obsolete nitrogen compound metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0019333	biological_process	denitrification pathway
A	0030288	cellular_component	outer membrane-bounded periplasmic space
A	0042128	biological_process	nitrate assimilation
A	0042597	cellular_component	periplasmic space
A	0046872	molecular_function	metal ion binding
A	0050421	molecular_function	nitrite reductase (NO-forming) activity
B	0004322	molecular_function	ferroxidase activity
B	0005507	molecular_function	copper ion binding
B	0005515	molecular_function	protein binding
B	0006807	biological_process	obsolete nitrogen compound metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0019333	biological_process	denitrification pathway
B	0030288	cellular_component	outer membrane-bounded periplasmic space
B	0042128	biological_process	nitrate assimilation
B	0042597	cellular_component	periplasmic space
B	0046872	molecular_function	metal ion binding
B	0050421	molecular_function	nitrite reductase (NO-forming) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU A 1337

Chain	Residue
A	HIS89
A	CYS130
A	HIS139
A	MET144

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CU A 1338

Chain	Residue
A	HOH2281
A	ASP92
A	HIS94
A	HIS129
A	HIS249
A	HIS300

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A 1339

Chain	Residue
A	ASP92
A	HIS94
A	HIS129
A	HIS300
A	HOH2281

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG A 1340

Chain	Residue
A	MET135
A	ALA194

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 1341

Chain	Residue
A	HIS165
A	ASP167
A	HOH2283
B	GLU195

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MES A 1342

Chain	Residue
A	ASP47
A	ASP48
A	LYS49
A	PHE306
A	GLU307
A	HOH2284
A	HOH2285
A	HOH2286

---

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE MES A 1343

Chain	Residue
A	PRO163
A	LEU164
A	HIS165
A	THR234
A	THR288
A	LYS290
A	PEG1347
A	HOH2287
A	HOH2288

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PG4 A 1344

Chain	Residue
A	ALA296
A	PHE306
A	HIS313
A	HOH2289

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN A 1345

Chain	Residue
A	ASP4
A	HIS8
A	HOH2290

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG A 1346

Chain	Residue
A	LYS10
A	VAL11
A	THR12
A	GLU34
A	THR36

---

site_id	BC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PEG A 1347

Chain	Residue
A	PRO163
A	LEU164
A	HIS165
A	MES1343
A	HOH2145
A	HOH2291
A	HOH2292
A	HOH2293
A	HOH2294
A	HOH2295
B	ALA191
B	THR192

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CU B 1337

Chain	Residue
B	HIS89
B	CYS130
B	HIS139
B	MET144

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1338

Chain	Residue
B	HIS94
B	HIS129
B	HIS300
B	HOH2308

---

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MES B 1339

Chain	Residue
B	PRO163
B	LEU164
B	HIS165
B	THR234
B	THR288
B	LYS290
B	HOH2309

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CU B 1340

Chain	Residue
B	ASP92
B	HIS94
B	HIS129
B	HIS249
B	HIS300
B	HOH2308

---

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG B 1341

Chain	Residue
B	PRO21
B	HIS22
B	GLU23

---

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG B 1342

Chain	Residue
B	VAL11
B	THR12
B	VAL14
B	PRO21

---

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG B 1343

Chain	Residue
B	VAL11
B	PHE35
B	GLN76
B	THR78
B	HOH2311

---

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1344

Chain	Residue
A	GLU195
B	HIS165
B	ASP167
B	HOH2312

---

site_id	CC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG B 1345

Chain	Residue
B	MET135
B	ALA194

---

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MES B 1346

Chain	Residue
B	ASP47
B	ASP48
B	LYS49
B	PHE306
B	HOH2149
B	HOH2314
B	HOH2315

---

site_id	CC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PG4 B 1347

Chain	Residue
B	GLU133
B	ALA296
B	PHE306
B	HIS313
B	HOH2146
B	HOH2316

---

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN B 1348

Chain	Residue
B	ASP4
B	HIS8
B	HOH2319

---