2XO6

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE Y132F MUTANT COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004803	molecular_function	transposase activity
A	0006310	biological_process	DNA recombination
A	0006313	biological_process	DNA transposition
A	0016787	molecular_function	hydrolase activity
A	0032196	biological_process	transposition
A	0046872	molecular_function	metal ion binding
D	0003677	molecular_function	DNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004803	molecular_function	transposase activity
D	0006310	biological_process	DNA recombination
D	0006313	biological_process	DNA transposition
D	0016787	molecular_function	hydrolase activity
D	0032196	biological_process	transposition
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CD A 1001

Chain	Residue
A	HIS67
A	HIS69
C	DT5
C	DG6
D	GLN136
D	HOH2106

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CD A 1002

Chain	Residue
B	HOH2069
B	HOH2072
B	HOH2073
B	HOH2074
B	HOH2075
B	HOH2076
B	HOH2077
A	PRO114
B	DC31
B	HOH2066

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CD A 1004

Chain	Residue
A	ASP41
A	HOH2030
A	HOH2032
A	HOH2034
A	HOH2035
B	DC27
B	HOH2054
B	HOH2055
B	HOH2056
B	HOH2057

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD A 1006

Chain	Residue
A	ASP66
A	HOH2017

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 1007

Chain	Residue
A	PRO7
A	GLU9
A	GLU38
A	GLU123

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CD D 1001

Chain	Residue
A	GLN136
D	MET64
D	HIS67
D	HIS69
F	DT5
F	DG6
F	HOH2010

---

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CD D 1002

Chain	Residue
D	PRO114
E	DC31
E	HOH2056
E	HOH2057
E	HOH2060
E	HOH2061
E	HOH2062
E	HOH2063
E	HOH2065
E	HOH2066

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CD D 1004

Chain	Residue
A	HIS137
D	ASP45
D	ASP49
D	HOH2038
D	HOH2039
D	HOH2045
D	HOH2046

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE CD E 1002

Chain	Residue
D	ASP41
D	HOH2028
D	HOH2029
D	HOH2032
D	HOH2033
E	DT26
E	HOH2041
E	HOH2042

---

site_id	BC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MG A 1141

Chain	Residue
A	PRO114
B	DC31
B	HOH2066
B	HOH2069
B	HOH2072
B	HOH2073
B	HOH2074
B	HOH2075
B	HOH2076
B	HOH2077

---

site_id	BC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MG A 1142

Chain	Residue
A	ASP41
A	HOH2030
A	HOH2032
A	HOH2034
A	HOH2035
B	DC27
B	HOH2054
B	HOH2055
B	HOH2056
B	HOH2057

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1143

Chain	Residue
A	PRO7
A	GLU9
A	GLU38
A	GLU123

---

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 1001

Chain	Residue
B	HOH2006
B	HOH2009
B	HOH2026
B	HOH2081
B	HOH2082
B	HOH2083

---

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG D 1137

Chain	Residue
D	HOH2045
D	HOH2046
A	HIS137
D	ASP45
D	ASP49
D	HOH2038
D	HOH2039

---

site_id	BC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT D 1138

Chain	Residue
D	TRP107

---

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG E 1001

Chain	Residue
E	HOH2003
E	HOH2022
E	HOH2072
E	HOH2074
E	HOH2075
E	HOH2078

---

site_id	BC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MG E 1038

Chain	Residue
D	PRO114
E	DC31
E	HOH2056
E	HOH2057
E	HOH2060
E	HOH2061
E	HOH2062
E	HOH2063
E	HOH2065
E	HOH2066

---

site_id	BC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MG E 1039

Chain	Residue
D	ASP41
D	HOH2028
D	HOH2029
D	HOH2032
D	HOH2033
E	DT26
E	HOH2041
E	HOH2042

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Region: {"description":"Mobile alpha helix","evidences":[{"source":"PubMed","id":"20890269","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"20890269","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20890269","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Critical for recognizing and stabilizing G:T mismatch in DNA substrate","evidences":[{"source":"PubMed","id":"20890269","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---