2XAU
Crystal structure of the Prp43p DEAH-box RNA helicase in complex with ADP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000390 | biological_process | spliceosomal complex disassembly |
| A | 0000462 | biological_process | maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| A | 0000463 | biological_process | maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| A | 0000466 | biological_process | maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003724 | molecular_function | RNA helicase activity |
| A | 0003729 | molecular_function | mRNA binding |
| A | 0004386 | molecular_function | helicase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005681 | cellular_component | spliceosomal complex |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0006364 | biological_process | rRNA processing |
| A | 0006397 | biological_process | mRNA processing |
| A | 0008186 | molecular_function | ATP-dependent activity, acting on RNA |
| A | 0008380 | biological_process | RNA splicing |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0022613 | biological_process | ribonucleoprotein complex biogenesis |
| A | 0030490 | biological_process | maturation of SSU-rRNA |
| A | 0030686 | cellular_component | 90S preribosome |
| A | 0042273 | biological_process | ribosomal large subunit biogenesis |
| A | 0071014 | cellular_component | post-mRNA release spliceosomal complex |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000390 | biological_process | spliceosomal complex disassembly |
| B | 0000462 | biological_process | maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| B | 0000463 | biological_process | maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| B | 0000466 | biological_process | maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003724 | molecular_function | RNA helicase activity |
| B | 0003729 | molecular_function | mRNA binding |
| B | 0004386 | molecular_function | helicase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005681 | cellular_component | spliceosomal complex |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0006364 | biological_process | rRNA processing |
| B | 0006397 | biological_process | mRNA processing |
| B | 0008186 | molecular_function | ATP-dependent activity, acting on RNA |
| B | 0008380 | biological_process | RNA splicing |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016887 | molecular_function | ATP hydrolysis activity |
| B | 0022613 | biological_process | ribonucleoprotein complex biogenesis |
| B | 0030490 | biological_process | maturation of SSU-rRNA |
| B | 0030686 | cellular_component | 90S preribosome |
| B | 0042273 | biological_process | ribosomal large subunit biogenesis |
| B | 0071014 | cellular_component | post-mRNA release spliceosomal complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE ADP A 1750 |
| Chain | Residue |
| A | LEU93 |
| A | PHE357 |
| A | ASP386 |
| A | ARG430 |
| A | MG1751 |
| A | HOH2212 |
| A | HOH2219 |
| A | HOH2465 |
| A | HOH2747 |
| A | HOH2748 |
| A | HOH2749 |
| A | THR118 |
| A | HOH2750 |
| A | HOH2751 |
| A | GLY119 |
| A | SER120 |
| A | GLY121 |
| A | LYS122 |
| A | THR123 |
| A | THR124 |
| A | ARG159 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 1751 |
| Chain | Residue |
| A | THR123 |
| A | ADP1750 |
| A | HOH2290 |
| A | HOH2462 |
| A | HOH2747 |
| A | HOH2750 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NI A 1752 |
| Chain | Residue |
| A | HIS479 |
| A | HIS606 |
| A | HOH2545 |
| A | HOH2648 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1753 |
| Chain | Residue |
| A | THR311 |
| A | GLY346 |
| A | HOH2456 |
| A | HOH2752 |
| A | HOH2753 |
| A | HOH2754 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1754 |
| Chain | Residue |
| A | LEU549 |
| A | VAL551 |
| A | THR709 |
| A | HOH2728 |
| A | HOH2755 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE ADP B 1748 |
| Chain | Residue |
| B | LEU93 |
| B | GLU117 |
| B | THR118 |
| B | GLY119 |
| B | SER120 |
| B | GLY121 |
| B | LYS122 |
| B | THR123 |
| B | THR124 |
| B | ARG159 |
| B | PHE357 |
| B | ASP386 |
| B | ARG430 |
| B | MG1749 |
| B | HOH2200 |
| B | HOH2207 |
| B | HOH2415 |
| B | HOH2418 |
| B | HOH2422 |
| B | HOH2655 |
| B | HOH2656 |
| B | HOH2657 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 1749 |
| Chain | Residue |
| B | THR123 |
| B | ADP1748 |
| B | HOH2188 |
| B | HOH2268 |
| B | HOH2415 |
| B | HOH2656 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NI B 1750 |
| Chain | Residue |
| B | HIS479 |
| B | HIS606 |
| B | HOH2570 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B 1751 |
| Chain | Residue |
| B | THR311 |
| B | TYR345 |
| B | GLY346 |
| B | HOH2658 |
| B | HOH2659 |
| B | HOH2660 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 1752 |
| Chain | Residue |
| B | LEU549 |
| B | VAL551 |
| B | THR709 |
| B | HOH2630 |
| B | HOH2631 |
| B | HOH2662 |
| B | HOH2663 |
Functional Information from PROSITE/UniProt
| site_id | PS00690 |
| Number of Residues | 10 |
| Details | DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ScIILDEAHE |
| Chain | Residue | Details |
| A | SER210-GLU219 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 330 |
| Details | Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 360 |
| Details | Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | Motif: {"description":"DEAH box"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 24 |
| Details | Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
