Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2XAU

Crystal structure of the Prp43p DEAH-box RNA helicase in complex with ADP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000390	biological_process	spliceosomal complex disassembly
A	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
A	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
A	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
A	0003676	molecular_function	nucleic acid binding
A	0003689	molecular_function	DNA clamp loader activity
A	0003723	molecular_function	RNA binding
A	0003724	molecular_function	RNA helicase activity
A	0003729	molecular_function	mRNA binding
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005681	cellular_component	spliceosomal complex
A	0005730	cellular_component	nucleolus
A	0005739	cellular_component	mitochondrion
A	0006338	biological_process	chromatin remodeling
A	0006364	biological_process	rRNA processing
A	0006396	biological_process	RNA processing
A	0006397	biological_process	mRNA processing
A	0008186	molecular_function	ATP-dependent activity, acting on RNA
A	0008380	biological_process	RNA splicing
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0022613	biological_process	ribonucleoprotein complex biogenesis
A	0030490	biological_process	maturation of SSU-rRNA
A	0030686	cellular_component	90S preribosome
A	0032040	cellular_component	small-subunit processome
A	0042273	biological_process	ribosomal large subunit biogenesis
A	0061775	molecular_function	cohesin loader activity
A	0071014	cellular_component	post-mRNA release spliceosomal complex
A	0140584	molecular_function	chromatin extrusion motor activity
A	0140588	biological_process	chromatin looping
A	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
A	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
A	1990904	cellular_component	ribonucleoprotein complex
B	0000390	biological_process	spliceosomal complex disassembly
B	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
B	0000463	biological_process	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
B	0000466	biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
B	0003676	molecular_function	nucleic acid binding
B	0003689	molecular_function	DNA clamp loader activity
B	0003723	molecular_function	RNA binding
B	0003724	molecular_function	RNA helicase activity
B	0003729	molecular_function	mRNA binding
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005681	cellular_component	spliceosomal complex
B	0005730	cellular_component	nucleolus
B	0005739	cellular_component	mitochondrion
B	0006338	biological_process	chromatin remodeling
B	0006364	biological_process	rRNA processing
B	0006396	biological_process	RNA processing
B	0006397	biological_process	mRNA processing
B	0008186	molecular_function	ATP-dependent activity, acting on RNA
B	0008380	biological_process	RNA splicing
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0022613	biological_process	ribonucleoprotein complex biogenesis
B	0030490	biological_process	maturation of SSU-rRNA
B	0030686	cellular_component	90S preribosome
B	0032040	cellular_component	small-subunit processome
B	0042273	biological_process	ribosomal large subunit biogenesis
B	0061775	molecular_function	cohesin loader activity
B	0071014	cellular_component	post-mRNA release spliceosomal complex
B	0140584	molecular_function	chromatin extrusion motor activity
B	0140588	biological_process	chromatin looping
B	0140665	molecular_function	ATP-dependent H3-H4 histone complex chaperone activity
B	0140849	molecular_function	ATP-dependent H2AZ histone chaperone activity
B	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ADP A 1750

Chain	Residue
A	LEU93
A	PHE357
A	ASP386
A	ARG430
A	MG1751
A	HOH2212
A	HOH2219
A	HOH2465
A	HOH2747
A	HOH2748
A	HOH2749
A	THR118
A	HOH2750
A	HOH2751
A	GLY119
A	SER120
A	GLY121
A	LYS122
A	THR123
A	THR124
A	ARG159

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 1751

Chain	Residue
A	THR123
A	ADP1750
A	HOH2290
A	HOH2462
A	HOH2747
A	HOH2750

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NI A 1752

Chain	Residue
A	HIS479
A	HIS606
A	HOH2545
A	HOH2648

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT A 1753

Chain	Residue
A	THR311
A	GLY346
A	HOH2456
A	HOH2752
A	HOH2753
A	HOH2754

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 1754

Chain	Residue
A	LEU549
A	VAL551
A	THR709
A	HOH2728
A	HOH2755

site_id	AC6
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ADP B 1748

Chain	Residue
B	LEU93
B	GLU117
B	THR118
B	GLY119
B	SER120
B	GLY121
B	LYS122
B	THR123
B	THR124
B	ARG159
B	PHE357
B	ASP386
B	ARG430
B	MG1749
B	HOH2200
B	HOH2207
B	HOH2415
B	HOH2418
B	HOH2422
B	HOH2655
B	HOH2656
B	HOH2657

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 1749

Chain	Residue
B	THR123
B	ADP1748
B	HOH2188
B	HOH2268
B	HOH2415
B	HOH2656

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE NI B 1750

Chain	Residue
B	HIS479
B	HIS606
B	HOH2570

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT B 1751

Chain	Residue
B	THR311
B	TYR345
B	GLY346
B	HOH2658
B	HOH2659
B	HOH2660

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 1752

Chain	Residue
B	LEU549
B	VAL551
B	THR709
B	HOH2630
B	HOH2631
B	HOH2662
B	HOH2663

Functional Information from PROSITE/UniProt

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ScIILDEAHE

Chain	Residue	Details
A	SER210-GLU219

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00541

Chain	Residue	Details
A	GLY116
B	GLY116

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER8
A	SER9
B	SER8
B	SER9

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)