2WOQ

Porphobilinogen Synthase (HemB) in Complex with 5-acetamido-4- oxohexanoic acid (Alaremycin 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004655	molecular_function	porphobilinogen synthase activity
A	0005829	cellular_component	cytosol
A	0006779	biological_process	porphyrin-containing compound biosynthetic process
A	0006782	biological_process	protoporphyrinogen IX biosynthetic process
A	0006783	biological_process	heme biosynthetic process
A	0033014	biological_process	tetrapyrrole biosynthetic process
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE AYC A 1333

Chain	Residue
A	ASP131
A	SER286
A	TYR324
A	HOH2085
A	HOH2173
A	SER175
A	TYR202
A	LYS205
A	TYR211
A	PHE214
A	LYS260
A	TYR283
A	VAL285

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1334

Chain	Residue
A	HOH2025
A	HOH2059
A	HOH2167
A	HOH2169

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 1335

Chain	Residue
A	GLU245
A	HOH2107
A	HOH2108
A	HOH2137
A	HOH2139
A	HOH2140

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1336

Chain	Residue
A	ASP131
A	ASP139
A	ASP176
A	HOH2090
A	HOH2105

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 1337

Chain	Residue
A	LEU27
A	ASP37
A	ASP319
A	HOH2018
A	HOH2022
A	HOH2031

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE A 1338

Chain	Residue
A	GLU30
A	GLU30
A	ASN31
A	ASN31
A	VAL32
A	VAL32
A	HOH2174

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE A 1339

Chain	Residue
A	ARG20
A	TYR147
A	GLY193
A	THR195
A	ASN196
A	HOH2092
A	HOH2118

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PE5 A 1340

Chain	Residue
A	TYR10
A	TYR12
A	GLU190

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PE5 A 1341

Chain	Residue
A	ILE141
A	PE51342
A	PE51342
A	HOH2176
A	HOH2176

---

site_id	BC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PE5 A 1342

Chain	Residue
A	ASP97
A	ASP97
A	VAL152
A	VAL152
A	ASP155
A	PE51341
A	PE51341
A	HOH2175
A	HOH2175
A	HOH2176
A	HOH2176

---

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE PE5 A 1343

Chain	Residue
A	ALA99
A	TYR102
A	ASN103
A	PRO104
A	ASP155
A	VAL158
A	ARG159
A	HOH2065

---

Functional Information from PROSITE/UniProt

site_id	PS00169
Number of Residues	13
Details	D_ALA_DEHYDRATASE Delta-aminolevulinic acid dehydratase active site. GaDmVMVKPGmpY

Chain	Residue	Details
A	GLY253-TYR265

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Schiff-base intermediate with substrate","evidences":[{"source":"PubMed","id":"12079382","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16819823","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10356331","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12079382","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1h7o

Chain	Residue	Details
A	SER175
A	ASP127
A	LYS260
A	LYS205

---

site_id	MCSA1
Number of Residues	2
Details	M-CSA 230

Chain	Residue	Details
A	LYS205	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor
A	LYS260	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor

---