2WOQ
Porphobilinogen Synthase (HemB) in Complex with 5-acetamido-4- oxohexanoic acid (Alaremycin 2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004655 | molecular_function | porphobilinogen synthase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006779 | biological_process | porphyrin-containing compound biosynthetic process |
| A | 0006782 | biological_process | protoporphyrinogen IX biosynthetic process |
| A | 0006783 | biological_process | heme biosynthetic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016829 | molecular_function | lyase activity |
| A | 0033014 | biological_process | tetrapyrrole biosynthetic process |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE AYC A 1333 |
| Chain | Residue |
| A | ASP131 |
| A | SER286 |
| A | TYR324 |
| A | HOH2085 |
| A | HOH2173 |
| A | SER175 |
| A | TYR202 |
| A | LYS205 |
| A | TYR211 |
| A | PHE214 |
| A | LYS260 |
| A | TYR283 |
| A | VAL285 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 1334 |
| Chain | Residue |
| A | HOH2025 |
| A | HOH2059 |
| A | HOH2167 |
| A | HOH2169 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 1335 |
| Chain | Residue |
| A | GLU245 |
| A | HOH2107 |
| A | HOH2108 |
| A | HOH2137 |
| A | HOH2139 |
| A | HOH2140 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 1336 |
| Chain | Residue |
| A | ASP131 |
| A | ASP139 |
| A | ASP176 |
| A | HOH2090 |
| A | HOH2105 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 1337 |
| Chain | Residue |
| A | LEU27 |
| A | ASP37 |
| A | ASP319 |
| A | HOH2018 |
| A | HOH2022 |
| A | HOH2031 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EPE A 1338 |
| Chain | Residue |
| A | GLU30 |
| A | GLU30 |
| A | ASN31 |
| A | ASN31 |
| A | VAL32 |
| A | VAL32 |
| A | HOH2174 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EPE A 1339 |
| Chain | Residue |
| A | ARG20 |
| A | TYR147 |
| A | GLY193 |
| A | THR195 |
| A | ASN196 |
| A | HOH2092 |
| A | HOH2118 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PE5 A 1340 |
| Chain | Residue |
| A | TYR10 |
| A | TYR12 |
| A | GLU190 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PE5 A 1341 |
| Chain | Residue |
| A | ILE141 |
| A | PE51342 |
| A | PE51342 |
| A | HOH2176 |
| A | HOH2176 |
| site_id | BC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PE5 A 1342 |
| Chain | Residue |
| A | ASP97 |
| A | ASP97 |
| A | VAL152 |
| A | VAL152 |
| A | ASP155 |
| A | PE51341 |
| A | PE51341 |
| A | HOH2175 |
| A | HOH2175 |
| A | HOH2176 |
| A | HOH2176 |
| site_id | BC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PE5 A 1343 |
| Chain | Residue |
| A | ALA99 |
| A | TYR102 |
| A | ASN103 |
| A | PRO104 |
| A | ASP155 |
| A | VAL158 |
| A | ARG159 |
| A | HOH2065 |
Functional Information from PROSITE/UniProt
| site_id | PS00169 |
| Number of Residues | 13 |
| Details | D_ALA_DEHYDRATASE Delta-aminolevulinic acid dehydratase active site. GaDmVMVKPGmpY |
| Chain | Residue | Details |
| A | GLY253-TYR265 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Schiff-base intermediate with substrate => ECO:0000269|PubMed:12079382, ECO:0000269|PubMed:16819823 |
| Chain | Residue | Details |
| A | LYS205 | |
| A | LYS260 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10356331, ECO:0000269|PubMed:12079382 |
| Chain | Residue | Details |
| A | ARG215 | |
| A | LYS229 | |
| A | GLU245 | |
| A | SER286 | |
| A | TYR324 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1h7o |
| Chain | Residue | Details |
| A | SER175 | |
| A | ASP127 | |
| A | LYS260 | |
| A | LYS205 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 230 |
| Chain | Residue | Details |
| A | LYS205 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
| A | LYS260 | covalently attached, electron pair acceptor, electron pair donor, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
