2WKI

Crystal structure of the OXA-10 K70C mutant at pH 7.0

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005886	cellular_component	plasma membrane
A	0008658	molecular_function	penicillin binding
A	0008800	molecular_function	beta-lactamase activity
A	0016787	molecular_function	hydrolase activity
A	0042597	cellular_component	periplasmic space
A	0046677	biological_process	response to antibiotic
A	0071555	biological_process	cell wall organization
B	0005886	cellular_component	plasma membrane
B	0008658	molecular_function	penicillin binding
B	0008800	molecular_function	beta-lactamase activity
B	0016787	molecular_function	hydrolase activity
B	0042597	cellular_component	periplasmic space
B	0046677	biological_process	response to antibiotic
B	0071555	biological_process	cell wall organization

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 1265

Chain	Residue
A	SER115
A	THR206
A	GLY207
A	PHE208
A	ARG250
A	HOH2145
A	HOH2146

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 1266

Chain	Residue
A	ARG160
A	HOH2147

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 1267

Chain	Residue
A	LYS134
A	LYS138
A	LYS152
A	HOH2085

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 1268

Chain	Residue
A	GLU199
A	GLU227
A	GLU229
A	GOL1269
A	HOH2148
B	THR107
B	ARG109
B	GLY110

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 1269

Chain	Residue
A	PRO198
A	GLU199
A	GLU229
A	GOL1268
B	ARG104
B	LEU106
B	GLY110
B	VAL114

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 1270

Chain	Residue
A	SER21
A	SER48
A	VAL164
A	GLU168
A	HOH2149

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 1271

Chain	Residue
A	ALA98
A	GLU129
A	ILE146
A	GLY149
A	ILE150

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1272

Chain	Residue
A	ARG125
A	GLY128
A	GLU129
A	HOH2064
A	HOH2069
A	HOH2150

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1273

Chain	Residue
A	PHE139
A	GLU168
A	PHE169
A	SER172
A	HOH2151
A	HOH2152

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 1274

Chain	Residue
A	THR194
A	GLU195
A	TRP222
A	LYS251
A	HOH2111

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1275

Chain	Residue
A	ARG131
A	LYS134
A	TYR135
A	LYS152
A	GLU156
A	HOH2031

---

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 1266

Chain	Residue
B	SER115
B	THR206
B	GLY207
B	PHE208
B	ARG250
B	HOH2128
B	HOH2129

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL B 1267

Chain	Residue
B	ARG131
B	LYS134
B	TYR135
B	HOH2130

---

site_id	BC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL B 1268

Chain	Residue
A	THR107
A	ARG109
A	GLY110
B	GLU199
B	TYR200
B	LEU201
B	GLU227
B	GLU229
B	HOH2099
B	HOH2108
B	HOH2131

---

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL B 1269

Chain	Residue
B	ARG250
B	LYS251
B	HOH2132

---

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO B 1270

Chain	Residue
B	PHE139
B	GLU168
B	SER172
B	HOH2133

---

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO B 1271

Chain	Residue
B	THR194
B	TRP222
B	LYS251
B	HOH2134

---

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 1272

Chain	Residue
B	HOH2126
B	LYS45
B	TYR233
B	GLU261
B	ILE263

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Acyl-ester intermediate","evidences":[{"source":"PubMed","id":"11724923","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19860471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1K54","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WGI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19860471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2WGI","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"PubMed","id":"11188693","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11724923","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19860471","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E4D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K4E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K4F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K54","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K55","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K56","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K57","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K6S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2RL3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---