Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2WCD

Crystal structure of the assembled cytolysin A pore

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0016020	cellular_component	membrane
A	0020002	cellular_component	host cell plasma membrane
A	0031640	biological_process	killing of cells of another organism
A	0033644	cellular_component	host cell membrane
A	0035821	biological_process	modulation of process of another organism
A	0042597	cellular_component	periplasmic space
A	0042802	molecular_function	identical protein binding
A	0044179	biological_process	hemolysis in another organism
A	0090729	molecular_function	toxin activity
B	0005576	cellular_component	extracellular region
B	0016020	cellular_component	membrane
B	0020002	cellular_component	host cell plasma membrane
B	0031640	biological_process	killing of cells of another organism
B	0033644	cellular_component	host cell membrane
B	0035821	biological_process	modulation of process of another organism
B	0042597	cellular_component	periplasmic space
B	0042802	molecular_function	identical protein binding
B	0044179	biological_process	hemolysis in another organism
B	0090729	molecular_function	toxin activity
C	0005576	cellular_component	extracellular region
C	0016020	cellular_component	membrane
C	0020002	cellular_component	host cell plasma membrane
C	0031640	biological_process	killing of cells of another organism
C	0033644	cellular_component	host cell membrane
C	0035821	biological_process	modulation of process of another organism
C	0042597	cellular_component	periplasmic space
C	0042802	molecular_function	identical protein binding
C	0044179	biological_process	hemolysis in another organism
C	0090729	molecular_function	toxin activity
D	0005576	cellular_component	extracellular region
D	0016020	cellular_component	membrane
D	0020002	cellular_component	host cell plasma membrane
D	0031640	biological_process	killing of cells of another organism
D	0033644	cellular_component	host cell membrane
D	0035821	biological_process	modulation of process of another organism
D	0042597	cellular_component	periplasmic space
D	0042802	molecular_function	identical protein binding
D	0044179	biological_process	hemolysis in another organism
D	0090729	molecular_function	toxin activity
E	0005576	cellular_component	extracellular region
E	0016020	cellular_component	membrane
E	0020002	cellular_component	host cell plasma membrane
E	0031640	biological_process	killing of cells of another organism
E	0033644	cellular_component	host cell membrane
E	0035821	biological_process	modulation of process of another organism
E	0042597	cellular_component	periplasmic space
E	0042802	molecular_function	identical protein binding
E	0044179	biological_process	hemolysis in another organism
E	0090729	molecular_function	toxin activity
F	0005576	cellular_component	extracellular region
F	0016020	cellular_component	membrane
F	0020002	cellular_component	host cell plasma membrane
F	0031640	biological_process	killing of cells of another organism
F	0033644	cellular_component	host cell membrane
F	0035821	biological_process	modulation of process of another organism
F	0042597	cellular_component	periplasmic space
F	0042802	molecular_function	identical protein binding
F	0044179	biological_process	hemolysis in another organism
F	0090729	molecular_function	toxin activity
G	0005576	cellular_component	extracellular region
G	0016020	cellular_component	membrane
G	0020002	cellular_component	host cell plasma membrane
G	0031640	biological_process	killing of cells of another organism
G	0033644	cellular_component	host cell membrane
G	0035821	biological_process	modulation of process of another organism
G	0042597	cellular_component	periplasmic space
G	0042802	molecular_function	identical protein binding
G	0044179	biological_process	hemolysis in another organism
G	0090729	molecular_function	toxin activity
H	0005576	cellular_component	extracellular region
H	0016020	cellular_component	membrane
H	0020002	cellular_component	host cell plasma membrane
H	0031640	biological_process	killing of cells of another organism
H	0033644	cellular_component	host cell membrane
H	0035821	biological_process	modulation of process of another organism
H	0042597	cellular_component	periplasmic space
H	0042802	molecular_function	identical protein binding
H	0044179	biological_process	hemolysis in another organism
H	0090729	molecular_function	toxin activity
I	0005576	cellular_component	extracellular region
I	0016020	cellular_component	membrane
I	0020002	cellular_component	host cell plasma membrane
I	0031640	biological_process	killing of cells of another organism
I	0033644	cellular_component	host cell membrane
I	0035821	biological_process	modulation of process of another organism
I	0042597	cellular_component	periplasmic space
I	0042802	molecular_function	identical protein binding
I	0044179	biological_process	hemolysis in another organism
I	0090729	molecular_function	toxin activity
J	0005576	cellular_component	extracellular region
J	0016020	cellular_component	membrane
J	0020002	cellular_component	host cell plasma membrane
J	0031640	biological_process	killing of cells of another organism
J	0033644	cellular_component	host cell membrane
J	0035821	biological_process	modulation of process of another organism
J	0042597	cellular_component	periplasmic space
J	0042802	molecular_function	identical protein binding
J	0044179	biological_process	hemolysis in another organism
J	0090729	molecular_function	toxin activity
K	0005576	cellular_component	extracellular region
K	0016020	cellular_component	membrane
K	0020002	cellular_component	host cell plasma membrane
K	0031640	biological_process	killing of cells of another organism
K	0033644	cellular_component	host cell membrane
K	0035821	biological_process	modulation of process of another organism
K	0042597	cellular_component	periplasmic space
K	0042802	molecular_function	identical protein binding
K	0044179	biological_process	hemolysis in another organism
K	0090729	molecular_function	toxin activity
L	0005576	cellular_component	extracellular region
L	0016020	cellular_component	membrane
L	0020002	cellular_component	host cell plasma membrane
L	0031640	biological_process	killing of cells of another organism
L	0033644	cellular_component	host cell membrane
L	0035821	biological_process	modulation of process of another organism
L	0042597	cellular_component	periplasmic space
L	0042802	molecular_function	identical protein binding
L	0044179	biological_process	hemolysis in another organism
L	0090729	molecular_function	toxin activity
M	0005576	cellular_component	extracellular region
M	0016020	cellular_component	membrane
M	0020002	cellular_component	host cell plasma membrane
M	0031640	biological_process	killing of cells of another organism
M	0033644	cellular_component	host cell membrane
M	0035821	biological_process	modulation of process of another organism
M	0042597	cellular_component	periplasmic space
M	0042802	molecular_function	identical protein binding
M	0044179	biological_process	hemolysis in another organism
M	0090729	molecular_function	toxin activity
N	0005576	cellular_component	extracellular region
N	0016020	cellular_component	membrane
N	0020002	cellular_component	host cell plasma membrane
N	0031640	biological_process	killing of cells of another organism
N	0033644	cellular_component	host cell membrane
N	0035821	biological_process	modulation of process of another organism
N	0042597	cellular_component	periplasmic space
N	0042802	molecular_function	identical protein binding
N	0044179	biological_process	hemolysis in another organism
N	0090729	molecular_function	toxin activity
O	0005576	cellular_component	extracellular region
O	0016020	cellular_component	membrane
O	0020002	cellular_component	host cell plasma membrane
O	0031640	biological_process	killing of cells of another organism
O	0033644	cellular_component	host cell membrane
O	0035821	biological_process	modulation of process of another organism
O	0042597	cellular_component	periplasmic space
O	0042802	molecular_function	identical protein binding
O	0044179	biological_process	hemolysis in another organism
O	0090729	molecular_function	toxin activity
P	0005576	cellular_component	extracellular region
P	0016020	cellular_component	membrane
P	0020002	cellular_component	host cell plasma membrane
P	0031640	biological_process	killing of cells of another organism
P	0033644	cellular_component	host cell membrane
P	0035821	biological_process	modulation of process of another organism
P	0042597	cellular_component	periplasmic space
P	0042802	molecular_function	identical protein binding
P	0044179	biological_process	hemolysis in another organism
P	0090729	molecular_function	toxin activity
Q	0005576	cellular_component	extracellular region
Q	0016020	cellular_component	membrane
Q	0020002	cellular_component	host cell plasma membrane
Q	0031640	biological_process	killing of cells of another organism
Q	0033644	cellular_component	host cell membrane
Q	0035821	biological_process	modulation of process of another organism
Q	0042597	cellular_component	periplasmic space
Q	0042802	molecular_function	identical protein binding
Q	0044179	biological_process	hemolysis in another organism
Q	0090729	molecular_function	toxin activity
R	0005576	cellular_component	extracellular region
R	0016020	cellular_component	membrane
R	0020002	cellular_component	host cell plasma membrane
R	0031640	biological_process	killing of cells of another organism
R	0033644	cellular_component	host cell membrane
R	0035821	biological_process	modulation of process of another organism
R	0042597	cellular_component	periplasmic space
R	0042802	molecular_function	identical protein binding
R	0044179	biological_process	hemolysis in another organism
R	0090729	molecular_function	toxin activity
S	0005576	cellular_component	extracellular region
S	0016020	cellular_component	membrane
S	0020002	cellular_component	host cell plasma membrane
S	0031640	biological_process	killing of cells of another organism
S	0033644	cellular_component	host cell membrane
S	0035821	biological_process	modulation of process of another organism
S	0042597	cellular_component	periplasmic space
S	0042802	molecular_function	identical protein binding
S	0044179	biological_process	hemolysis in another organism
S	0090729	molecular_function	toxin activity
T	0005576	cellular_component	extracellular region
T	0016020	cellular_component	membrane
T	0020002	cellular_component	host cell plasma membrane
T	0031640	biological_process	killing of cells of another organism
T	0033644	cellular_component	host cell membrane
T	0035821	biological_process	modulation of process of another organism
T	0042597	cellular_component	periplasmic space
T	0042802	molecular_function	identical protein binding
T	0044179	biological_process	hemolysis in another organism
T	0090729	molecular_function	toxin activity
U	0005576	cellular_component	extracellular region
U	0016020	cellular_component	membrane
U	0020002	cellular_component	host cell plasma membrane
U	0031640	biological_process	killing of cells of another organism
U	0033644	cellular_component	host cell membrane
U	0035821	biological_process	modulation of process of another organism
U	0042597	cellular_component	periplasmic space
U	0042802	molecular_function	identical protein binding
U	0044179	biological_process	hemolysis in another organism
U	0090729	molecular_function	toxin activity
V	0005576	cellular_component	extracellular region
V	0016020	cellular_component	membrane
V	0020002	cellular_component	host cell plasma membrane
V	0031640	biological_process	killing of cells of another organism
V	0033644	cellular_component	host cell membrane
V	0035821	biological_process	modulation of process of another organism
V	0042597	cellular_component	periplasmic space
V	0042802	molecular_function	identical protein binding
V	0044179	biological_process	hemolysis in another organism
V	0090729	molecular_function	toxin activity
W	0005576	cellular_component	extracellular region
W	0016020	cellular_component	membrane
W	0020002	cellular_component	host cell plasma membrane
W	0031640	biological_process	killing of cells of another organism
W	0033644	cellular_component	host cell membrane
W	0035821	biological_process	modulation of process of another organism
W	0042597	cellular_component	periplasmic space
W	0042802	molecular_function	identical protein binding
W	0044179	biological_process	hemolysis in another organism
W	0090729	molecular_function	toxin activity
X	0005576	cellular_component	extracellular region
X	0016020	cellular_component	membrane
X	0020002	cellular_component	host cell plasma membrane
X	0031640	biological_process	killing of cells of another organism
X	0033644	cellular_component	host cell membrane
X	0035821	biological_process	modulation of process of another organism
X	0042597	cellular_component	periplasmic space
X	0042802	molecular_function	identical protein binding
X	0044179	biological_process	hemolysis in another organism
X	0090729	molecular_function	toxin activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC A 304

Chain	Residue
A	TRP86
A	CYS87
A	THR91
A	LEU94
A	MET281
A	CYS285

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC A 305

Chain	Residue
A	CYS285

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC B 304

Chain	Residue
B	THR91
B	LEU94
B	MET281
B	CYS285
B	TRP86
B	CYS87

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC B 305

Chain	Residue
B	CYS285

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC C 304

Chain	Residue
C	TRP86
C	CYS87
C	ALA90
C	THR91
C	LEU94
C	MET281
C	CYS285

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC C 305

Chain	Residue
C	CYS285

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC D 304

Chain	Residue
D	TRP86
D	CYS87
D	THR91
D	LEU94
D	MET281
D	CYS285

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EMC D 305

Chain	Residue
D	THR91
D	CYS285

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC E 304

Chain	Residue
E	TRP86
E	CYS87
E	THR91
E	LEU94
E	MET281
E	CYS285

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC E 305

Chain	Residue
E	CYS285

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC F 304

Chain	Residue
F	TRP86
F	CYS87
F	ALA90
F	THR91
F	LEU94
F	MET281
F	CYS285

site_id	BC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC F 305

Chain	Residue
F	CYS285

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC G 304

Chain	Residue
G	TRP86
G	CYS87
G	ALA90
G	THR91
G	LEU94
G	MET281
G	CYS285

site_id	BC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC G 305

Chain	Residue
G	CYS285

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC H 304

Chain	Residue
H	TRP86
H	CYS87
H	ALA90
H	THR91
H	LEU94
H	MET281
H	CYS285

site_id	BC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC H 305

Chain	Residue
H	CYS285

site_id	BC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC I 304

Chain	Residue
I	TRP86
I	CYS87
I	THR91
I	LEU94
I	MET281
I	CYS285

site_id	BC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC I 305

Chain	Residue
I	CYS285

site_id	CC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC J 304

Chain	Residue
J	TRP86
J	CYS87
J	ALA90
J	THR91
J	LEU94
J	MET281
J	CYS285

site_id	CC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC J 305

Chain	Residue
J	CYS285

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC K 304

Chain	Residue
K	TRP86
K	CYS87
K	ALA90
K	THR91
K	LEU94
K	MET281
K	CYS285

site_id	CC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC K 305

Chain	Residue
K	CYS285

site_id	CC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC L 304

Chain	Residue
L	TRP86
L	CYS87
L	ALA90
L	THR91
L	LEU94
L	MET281
L	CYS285

site_id	CC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EMC L 305

Chain	Residue
L	THR91
L	CYS285

site_id	CC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC M 304

Chain	Residue
M	TRP86
M	CYS87
M	THR91
M	LEU94
M	MET281
M	CYS285

site_id	CC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC M 305

Chain	Residue
M	CYS285

site_id	CC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC N 304

Chain	Residue
N	TRP86
N	CYS87
N	THR91
N	LEU94
N	MET281
N	CYS285

site_id	DC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC N 305

Chain	Residue
N	CYS285

site_id	DC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC O 304

Chain	Residue
O	TRP86
O	CYS87
O	ALA90
O	THR91
O	LEU94
O	MET281
O	CYS285

site_id	DC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC O 305

Chain	Residue
O	CYS285

site_id	DC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC P 304

Chain	Residue
P	TRP86
P	CYS87
P	THR91
P	LEU94
P	MET281
P	CYS285

site_id	DC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC P 305

Chain	Residue
P	CYS285

site_id	DC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC Q 304

Chain	Residue
Q	TRP86
Q	CYS87
Q	THR91
Q	LEU94
Q	MET281
Q	CYS285

site_id	DC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC Q 305

Chain	Residue
Q	CYS285

site_id	DC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC R 304

Chain	Residue
R	TRP86
R	CYS87
R	THR91
R	LEU94
R	MET281
R	CYS285

site_id	DC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC R 305

Chain	Residue
R	CYS285

site_id	EC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC S 304

Chain	Residue
S	TRP86
S	CYS87
S	ALA90
S	THR91
S	LEU94
S	MET281
S	CYS285

site_id	EC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC S 305

Chain	Residue
S	CYS285

site_id	EC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC T 304

Chain	Residue
T	TRP86
T	CYS87
T	ALA90
T	THR91
T	LEU94
T	MET281
T	CYS285

site_id	EC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EMC T 305

Chain	Residue
T	THR91
T	CYS285

site_id	EC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EMC U 304

Chain	Residue
U	TRP86
U	CYS87
U	ALA90
U	THR91
U	LEU94
U	MET281
U	CYS285

site_id	EC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC U 305

Chain	Residue
U	CYS285

site_id	EC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC V 304

Chain	Residue
V	TRP86
V	CYS87
V	THR91
V	LEU94
V	MET281
V	CYS285

site_id	EC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC V 305

Chain	Residue
V	CYS285

site_id	EC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC W 304

Chain	Residue
W	TRP86
W	CYS87
W	THR91
W	LEU94
W	MET281
W	CYS285

site_id	FC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EMC W 305

Chain	Residue
W	THR91
W	CYS285

site_id	FC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EMC X 304

Chain	Residue
X	TRP86
X	CYS87
X	THR91
X	LEU94
X	MET281
X	CYS285

site_id	FC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EMC X 305

Chain	Residue
X	CYS285

Functional Information from PROSITE/UniProt

site_id	PS00136
Number of Residues	12
Details	SUBTILASE_ASP Serine proteases, subtilase family, aspartic acid active site. IKVLDDGItklN

Chain	Residue	Details
A	ILE117-ASN128

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	480
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
A	ALA183-VAL203
J	ALA183-VAL203
K	ALA183-VAL203
L	ALA183-VAL203
M	ALA183-VAL203
N	ALA183-VAL203
O	ALA183-VAL203
P	ALA183-VAL203
Q	ALA183-VAL203
R	ALA183-VAL203
S	ALA183-VAL203
B	ALA183-VAL203
T	ALA183-VAL203
U	ALA183-VAL203
V	ALA183-VAL203
W	ALA183-VAL203
X	ALA183-VAL203
C	ALA183-VAL203
D	ALA183-VAL203
E	ALA183-VAL203
F	ALA183-VAL203
G	ALA183-VAL203
H	ALA183-VAL203
I	ALA183-VAL203

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)