Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2V69

Crystal structure of Chlamydomonas reinhardtii Rubisco with a large- subunit mutation D473E

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004497	molecular_function	monooxygenase activity
A	0009507	cellular_component	chloroplast
A	0009853	biological_process	photorespiration
A	0015977	biological_process	carbon fixation
A	0015979	biological_process	photosynthesis
A	0016491	molecular_function	oxidoreductase activity
A	0016829	molecular_function	lyase activity
A	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004497	molecular_function	monooxygenase activity
B	0009507	cellular_component	chloroplast
B	0009853	biological_process	photorespiration
B	0015977	biological_process	carbon fixation
B	0015979	biological_process	photosynthesis
B	0016491	molecular_function	oxidoreductase activity
B	0016829	molecular_function	lyase activity
B	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
B	0019253	biological_process	reductive pentose-phosphate cycle
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004497	molecular_function	monooxygenase activity
C	0009507	cellular_component	chloroplast
C	0009853	biological_process	photorespiration
C	0015977	biological_process	carbon fixation
C	0015979	biological_process	photosynthesis
C	0016491	molecular_function	oxidoreductase activity
C	0016829	molecular_function	lyase activity
C	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004497	molecular_function	monooxygenase activity
D	0009507	cellular_component	chloroplast
D	0009853	biological_process	photorespiration
D	0015977	biological_process	carbon fixation
D	0015979	biological_process	photosynthesis
D	0016491	molecular_function	oxidoreductase activity
D	0016829	molecular_function	lyase activity
D	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
D	0019253	biological_process	reductive pentose-phosphate cycle
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004497	molecular_function	monooxygenase activity
E	0009507	cellular_component	chloroplast
E	0009853	biological_process	photorespiration
E	0015977	biological_process	carbon fixation
E	0015979	biological_process	photosynthesis
E	0016491	molecular_function	oxidoreductase activity
E	0016829	molecular_function	lyase activity
E	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
E	0019253	biological_process	reductive pentose-phosphate cycle
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004497	molecular_function	monooxygenase activity
F	0009507	cellular_component	chloroplast
F	0009853	biological_process	photorespiration
F	0015977	biological_process	carbon fixation
F	0015979	biological_process	photosynthesis
F	0016491	molecular_function	oxidoreductase activity
F	0016829	molecular_function	lyase activity
F	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
F	0019253	biological_process	reductive pentose-phosphate cycle
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0004497	molecular_function	monooxygenase activity
G	0009507	cellular_component	chloroplast
G	0009853	biological_process	photorespiration
G	0015977	biological_process	carbon fixation
G	0015979	biological_process	photosynthesis
G	0016491	molecular_function	oxidoreductase activity
G	0016829	molecular_function	lyase activity
G	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
G	0019253	biological_process	reductive pentose-phosphate cycle
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0004497	molecular_function	monooxygenase activity
H	0009507	cellular_component	chloroplast
H	0009853	biological_process	photorespiration
H	0015977	biological_process	carbon fixation
H	0015979	biological_process	photosynthesis
H	0016491	molecular_function	oxidoreductase activity
H	0016829	molecular_function	lyase activity
H	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
H	0019253	biological_process	reductive pentose-phosphate cycle
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A1470

Chain	Residue
A	LYS177
A	KCX201
A	ASP203
A	GLU204
A	CAP1471

site_id	AC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CAP A1471

Chain	Residue
A	ASP203
A	GLU204
A	HIS294
A	ARG295
A	HIS327
A	LYS334
A	LEU335
A	SER379
A	GLY380
A	GLY381
A	GLY403
A	GLY404
A	HOH1049
A	HOH1050
A	MG1470
B	GLU60
B	THR65
B	TRP66
B	ASN123
A	THR173
A	LYS175
A	LYS177
A	KCX201

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B1470

Chain	Residue
B	LYS177
B	KCX201
B	ASP203
B	GLU204
B	CAP1471

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE CAP B1471

Chain	Residue
A	GLU60
A	THR65
A	TRP66
A	ASN123
B	THR173
B	LYS175
B	LYS177
B	KCX201
B	ASP203
B	GLU204
B	HIS294
B	ARG295
B	HIS327
B	LYS334
B	LEU335
B	SER379
B	GLY380
B	GLY381
B	GLY403
B	GLY404
B	HOH1029
B	MG1470

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C1471

Chain	Residue
C	LYS177
C	KCX201
C	ASP203
C	GLU204
C	CAP1472

site_id	AC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CAP C1472

Chain	Residue
C	THR173
C	LYS175
C	LYS177
C	KCX201
C	ASP203
C	GLU204
C	HIS294
C	ARG295
C	HIS327
C	LYS334
C	LEU335
C	SER379
C	GLY380
C	GLY381
C	GLY403
C	GLY404
C	HOH1051
C	HOH1056
C	MG1471
D	GLU60
D	THR65
D	TRP66
D	ASN123

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D1475

Chain	Residue
D	LYS177
D	KCX201
D	ASP203
D	GLU204
D	CAP1476

site_id	AC8
Number of Residues	25
Details	BINDING SITE FOR RESIDUE CAP D1476

Chain	Residue
D	HIS294
D	ARG295
D	HIS327
D	LYS334
D	LEU335
D	SER379
D	GLY380
D	GLY381
D	GLY403
D	GLY404
D	HOH1029
D	HOH1035
D	HOH1036
D	MG1475
C	GLU60
C	THR65
C	TRP66
C	ASN123
C	HOH1015
D	THR173
D	LYS175
D	LYS177
D	KCX201
D	ASP203
D	GLU204

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E1470

Chain	Residue
E	LYS177
E	KCX201
E	ASP203
E	GLU204
E	CAP1471

site_id	BC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CAP E1471

Chain	Residue
E	THR173
E	LYS175
E	LYS177
E	KCX201
E	ASP203
E	GLU204
E	HIS294
E	ARG295
E	HIS327
E	LYS334
E	LEU335
E	SER379
E	GLY380
E	GLY381
E	GLY403
E	GLY404
E	HOH1048
E	HOH1049
E	MG1470
F	GLU60
F	THR65
F	TRP66
F	ASN123

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F1470

Chain	Residue
F	LYS177
F	KCX201
F	ASP203
F	GLU204
F	CAP1471

site_id	BC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CAP F1471

Chain	Residue
E	GLU60
E	THR65
E	TRP66
E	ASN123
F	THR173
F	LYS175
F	LYS177
F	KCX201
F	ASP203
F	GLU204
F	HIS294
F	ARG295
F	HIS327
F	LYS334
F	LEU335
F	SER379
F	GLY380
F	GLY381
F	GLY403
F	GLY404
F	HOH1039
F	HOH1040
F	MG1470

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG G1470

Chain	Residue
G	LYS177
G	KCX201
G	ASP203
G	GLU204
G	CAP1471

site_id	BC5
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CAP G1471

Chain	Residue
G	THR173
G	LYS175
G	LYS177
G	KCX201
G	ASP203
G	GLU204
G	HIS294
G	ARG295
G	HIS327
G	LYS334
G	LEU335
G	SER379
G	GLY380
G	GLY381
G	GLY403
G	GLY404
G	HOH1035
G	HOH1041
G	MG1470
H	GLU60
H	THR65
H	TRP66
H	ASN123

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG H1470

Chain	Residue
H	LYS177
H	KCX201
H	ASP203
H	GLU204
H	CAP1471

site_id	BC7
Number of Residues	22
Details	BINDING SITE FOR RESIDUE CAP H1471

Chain	Residue
G	GLU60
G	THR65
G	TRP66
G	ASN123
H	THR173
H	LYS175
H	LYS177
H	KCX201
H	ASP203
H	GLU204
H	HIS294
H	ARG295
H	HIS327
H	LYS334
H	LEU335
H	SER379
H	GLY380
H	GLY381
H	GLY403
H	GLY404
H	HOH1040
H	MG1470

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A1472

Chain	Residue
A	LYS18
A	ASP19
A	TRP66
A	THR67
A	THR68

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A1473

Chain	Residue
A	LYS466
A	PHE467
A	GLU468
A	PHE469

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO C1473

Chain	Residue
C	TYR24
C	THR68
C	VAL69
C	ASP72
C	HOH1057
C	EDO1474

site_id	CC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO C1474

Chain	Residue
C	LYS18
C	TYR20
C	THR65
C	TRP66
C	THR67
C	THR68
C	HOH1057
C	EDO1473

site_id	CC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO C1475

Chain	Residue
C	ARG295
C	GLU336
C	HOH1043

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO E1472

Chain	Residue
E	TYR24
E	THR68
E	VAL69
E	ASP72
E	HOH1053

site_id	CC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO E1473

Chain	Residue
E	LYS18
E	TYR20
E	THR65
E	TRP66
E	THR67
E	THR68
E	HOH1002

site_id	CC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO E1474

Chain	Residue
E	GLU52

site_id	CC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO G1472

Chain	Residue
G	TYR24
G	THR68
G	VAL69
G	ASP72

site_id	CC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO G1473

Chain	Residue
G	LYS18
G	ASP19
G	TYR20
G	THR65
G	TRP66
G	THR67
G	THR68

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO E1475

Chain	Residue
E	LEU270
E	HOH1054
F	LEU270
F	HOH1028

site_id	DC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A1474

Chain	Residue
A	LEU270
A	HOH1051
A	HOH1052
B	LEU270

site_id	DC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO H1472

Chain	Residue
G	LEU270
H	LEU270
H	HOH1041
H	HOH1042

site_id	DC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B1472

Chain	Residue
B	TYR24
B	GLY64
B	THR68
B	VAL69
B	ASP72
B	EDO1473

site_id	DC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO B1473

Chain	Residue
B	LYS18
B	TYR20
B	THR65
B	TRP66
B	THR67
B	THR68
B	EDO1472

site_id	DC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO H1473

Chain	Residue
H	TYR24
H	GLY64
H	THR68
H	VAL69
H	ASP72
H	HOH1043
H	EDO1474

site_id	DC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO H1474

Chain	Residue
H	LYS18
H	ASP19
H	TRP66
H	THR67
H	THR68
H	EDO1473

site_id	DC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO H1475

Chain	Residue
H	LYS466
H	PHE467
H	GLU468

site_id	DC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO F1472

Chain	Residue
F	TYR24
F	THR68
F	VAL69
F	ASP72
F	LEU77
F	HOH1041
F	EDO1473

site_id	DC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO F1473

Chain	Residue
F	LYS18
F	ASP19
F	THR65
F	TRP66
F	THR67
F	THR68
F	EDO1472

site_id	EC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO D1477

Chain	Residue
D	TYR24
D	GLY64
D	THR68
D	VAL69
D	ASP72
D	HOH1002

site_id	EC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO D1478

Chain	Residue
D	LYS18
D	ASP19
D	THR65
D	TRP66
D	THR67
D	THR68

site_id	EC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO D1479

Chain	Residue
C	LEU270
C	THR271
D	LEU270
D	GLY273
D	PHE274

site_id	EC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO G1474

Chain	Residue
E	ASP286
E	ASN287
E	HOH1039
G	ARG215
G	LYS252
G	SMC256
M	VAL63

Functional Information from PROSITE/UniProt

site_id	PS00157
Number of Residues	9
Details	RUBISCO_LARGE Ribulose bisphosphate carboxylase large chain active site. GlDFtKdDE

Chain	Residue	Details
A	GLY196-GLU204

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"description":"in homodimeric partner"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	40
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"description":"via carbamate group","evidences":[{"source":"PubMed","id":"11641402","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11866526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11641402","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11866526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	16
Details	Modified residue: {"description":"4-hydroxyproline","evidences":[{"source":"PubMed","id":"11641402","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11866526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"PubMed","id":"11641402","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11866526","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6302265","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	16
Details	Modified residue: {"description":"S-methylcysteine","evidences":[{"source":"PubMed","id":"11641402","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11866526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
A	LYS175
A	HIS294
A	LYS177
A	ASP203
A	HIS327

site_id	CSA2
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
B	LYS175
B	HIS294
B	LYS177
B	ASP203
B	HIS327

site_id	CSA3
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
C	LYS175
C	HIS294
C	LYS177
C	ASP203
C	HIS327

site_id	CSA4
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
D	LYS175
D	HIS294
D	LYS177
D	ASP203
D	HIS327

site_id	CSA5
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
E	LYS175
E	HIS294
E	LYS177
E	ASP203
E	HIS327

site_id	CSA6
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
F	LYS175
F	HIS294
F	LYS177
F	ASP203
F	HIS327

site_id	CSA7
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
G	LYS175
G	HIS294
G	LYS177
G	ASP203
G	HIS327

site_id	CSA8
Number of Residues	5
Details	Annotated By Reference To The Literature 1rbl

Chain	Residue	Details
H	LYS175
H	HIS294
H	LYS177
H	ASP203
H	HIS327

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)