2TAA

STRUCTURE AND POSSIBLE CATALYTIC RESIDUES OF TAKA-AMYLASE A

Replaces:  1TAA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004556	molecular_function	alpha-amylase activity
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0009277	cellular_component	fungal-type cell wall
A	0016052	biological_process	carbohydrate catabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0030287	cellular_component	cell wall-bounded periplasmic space
A	0030428	cellular_component	cell septum
A	0031521	cellular_component	spitzenkorper
A	0032163	cellular_component	hyphal septin band
A	0043169	molecular_function	cation binding
A	0046872	molecular_function	metal ion binding
B	0004556	molecular_function	alpha-amylase activity
B	0005509	molecular_function	calcium ion binding
B	0005576	cellular_component	extracellular region
B	0005975	biological_process	carbohydrate metabolic process
B	0009277	cellular_component	fungal-type cell wall
B	0016052	biological_process	carbohydrate catabolic process
B	0016787	molecular_function	hydrolase activity
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0030287	cellular_component	cell wall-bounded periplasmic space
B	0030428	cellular_component	cell septum
B	0031521	cellular_component	spitzenkorper
B	0032163	cellular_component	hyphal septin band
B	0043169	molecular_function	cation binding
B	0046872	molecular_function	metal ion binding
C	0004556	molecular_function	alpha-amylase activity
C	0005509	molecular_function	calcium ion binding
C	0005576	cellular_component	extracellular region
C	0005975	biological_process	carbohydrate metabolic process
C	0009277	cellular_component	fungal-type cell wall
C	0016052	biological_process	carbohydrate catabolic process
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0030287	cellular_component	cell wall-bounded periplasmic space
C	0030428	cellular_component	cell septum
C	0031521	cellular_component	spitzenkorper
C	0032163	cellular_component	hyphal septin band
C	0043169	molecular_function	cation binding
C	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA A 479

Chain	Residue
A	ASN121
A	GLU162
A	ASP175

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA B 479

Chain	Residue
B	ASN121
B	GLU162
B	ASP175

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CA C 479

Chain	Residue
C	ASN121
C	GLU162
C	ASP175

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16880540","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6609921","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"9283074","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","featureId":"CAR_000125","evidences":[{"source":"PubMed","id":"16880540","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP206
A	GLU230

---

site_id	CSA10
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ARG204
A	ASP206
A	HIS296
A	ASP297
A	GLU230

---

site_id	CSA11
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ARG204
B	ASP206
B	HIS296
B	ASP297
B	GLU230

---

site_id	CSA12
Number of Residues	5
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ARG204
C	ASP206
C	HIS296
C	ASP297
C	GLU230

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP206
B	GLU230

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP206
C	GLU230

---

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	HIS122
A	ASP206
A	ASP297
A	GLU230

---

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	HIS122
B	ASP206
B	ASP297
B	GLU230

---

site_id	CSA6
Number of Residues	4
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	HIS122
C	ASP206
C	ASP297
C	GLU230

---

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
A	ASP206
A	ASP297
A	GLU230

---

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
B	ASP206
B	ASP297
B	GLU230

---

site_id	CSA9
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
C	ASP206
C	ASP297
C	GLU230

---