2R2G
Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0009698 | biological_process | phenylpropanoid metabolic process |
| A | 0009699 | biological_process | phenylpropanoid biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0042855 | biological_process | eugenol biosynthetic process |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0009698 | biological_process | phenylpropanoid metabolic process |
| B | 0009699 | biological_process | phenylpropanoid biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0042855 | biological_process | eugenol biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAP A 401 |
| Chain | Residue |
| A | THR16 |
| A | LEU83 |
| A | PHE85 |
| A | GLN87 |
| A | SER110 |
| A | ASP111 |
| A | PHE112 |
| A | ASN152 |
| A | PHE154 |
| A | PHE158 |
| A | EMF601 |
| A | GLY17 |
| A | HOH1002 |
| A | HOH1015 |
| A | HOH1023 |
| A | HOH1041 |
| A | HOH1078 |
| A | HOH1086 |
| A | HOH1092 |
| A | HOH1126 |
| A | HOH1456 |
| A | TYR18 |
| A | ILE19 |
| A | THR38 |
| A | ARG39 |
| A | SER42 |
| A | LYS44 |
| A | ALA82 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE EMF A 601 |
| Chain | Residue |
| A | PHE85 |
| A | GLY113 |
| A | VAL114 |
| A | ASN152 |
| A | TYR157 |
| A | PHE158 |
| A | PRO258 |
| A | ILE261 |
| A | LEU262 |
| A | LEU265 |
| A | ALA312 |
| A | PHE314 |
| A | NAP401 |
| site_id | AC3 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAP B 401 |
| Chain | Residue |
| B | THR16 |
| B | GLY17 |
| B | TYR18 |
| B | ILE19 |
| B | THR38 |
| B | ARG39 |
| B | SER42 |
| B | LYS44 |
| B | ALA82 |
| B | LEU83 |
| B | ALA84 |
| B | PHE85 |
| B | GLN87 |
| B | SER110 |
| B | ASP111 |
| B | PHE112 |
| B | ASN152 |
| B | PHE154 |
| B | PHE158 |
| B | EMF602 |
| B | HOH1012 |
| B | HOH1018 |
| B | HOH1029 |
| B | HOH1032 |
| B | HOH1046 |
| B | HOH1061 |
| B | HOH1081 |
| B | HOH1094 |
| B | HOH1231 |
| B | HOH1465 |
| site_id | AC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE EMF B 602 |
| Chain | Residue |
| B | GLY113 |
| B | VAL114 |
| B | ASN152 |
| B | TYR157 |
| B | PHE158 |
| B | PRO258 |
| B | ILE261 |
| B | LEU262 |
| B | LEU265 |
| B | ALA312 |
| B | PHE314 |
| B | NAP401 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:17912370 |
| Chain | Residue | Details |
| A | GLN132 | |
| B | GLN132 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17912370, ECO:0000269|PubMed:18208524, ECO:0007744|PDB:2QW8, ECO:0007744|PDB:2QX7, ECO:0007744|PDB:2QZZ, ECO:0007744|PDB:2R2G, ECO:0007744|PDB:2R6J, ECO:0007744|PDB:3C3X |
| Chain | Residue | Details |
| A | THR16 | |
| A | THR38 | |
| A | SER110 | |
| A | ASN152 | |
| B | THR16 | |
| B | THR38 | |
| B | SER110 | |
| B | ASN152 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:D0VWT0 |
| Chain | Residue | Details |
| A | ARG39 | |
| B | ARG39 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17912370, ECO:0007744|PDB:2QW8, ECO:0007744|PDB:2QX7, ECO:0007744|PDB:2QZZ, ECO:0007744|PDB:2R2G, ECO:0007744|PDB:2R6J |
| Chain | Residue | Details |
| A | PHE85 | |
| B | PHE85 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:17912370, ECO:0007744|PDB:2QZZ, ECO:0007744|PDB:2R2G |
| Chain | Residue | Details |
| A | VAL114 | |
| B | VAL114 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17912370, ECO:0007744|PDB:2QW8, ECO:0007744|PDB:2QX7, ECO:0007744|PDB:2QZZ, ECO:0007744|PDB:2R6J |
| Chain | Residue | Details |
| A | GLN132 | |
| B | GLN132 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:17912370, ECO:0007744|PDB:2QZZ |
| Chain | Residue | Details |
| A | PRO258 | |
| B | PRO258 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | SITE: Confers substrate specificity => ECO:0000269|PubMed:18208524 |
| Chain | Residue | Details |
| A | PHE85 | |
| A | ILE88 | |
| B | PHE85 | |
| B | ILE88 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | SITE: Required for activity => ECO:0000269|PubMed:17912370 |
| Chain | Residue | Details |
| A | ILE261 | |
| B | ILE261 |
