2PXA

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH and GTPG

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE GTP A 401

Chain	Residue
A	LYS13
A	SER215
A	HOH444
A	HOH560
A	HOH619
B	ARG42
A	LEU16
A	ASN17
A	MET19
A	PHE24
A	ARG28
A	SER150
A	SER151
A	PRO152

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE GTP B 403

Chain	Residue
B	LYS13
B	LEU16
B	ASN17
B	MET19
B	PHE24
B	ARG28
B	SER150
B	SER151
B	PRO152
B	ARG213
B	SER215
B	HOH527
B	HOH592

---

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE SAH B 500

Chain	Residue
B	SER56
B	GLY58
B	GLY81
B	CYS82
B	GLY83
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	HIS110
B	GLU111
B	VAL130
B	ASP131
B	VAL132
B	PHE133
B	ASP146
B	HOH524
B	HOH563
B	HOH564
B	HOH602

---

site_id	AC4
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SAH A 500

Chain	Residue
A	SER56
A	GLY58
A	GLY81
A	CYS82
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	HIS110
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	ILE147
A	HOH448
A	HOH658

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS182
A	GLU218
B	LYS61
B	ASP146
B	LYS182
B	GLU218

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	SER56
B	SER56
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	ASP131
B	VAL132
B	ILE147
B	TYR220
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	ASP131
A	VAL132
A	ILE147
A	TYR220

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS13
B	PHE24
B	ARG28
B	SER150
B	ARG213
B	SER215
A	ASN17
A	PHE24
A	ARG28
A	SER150
A	ARG213
A	SER215
B	LYS13
B	ASN17

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU16
A	MET19
B	LEU16
B	MET19

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS182
A	GLU218
B	LYS61
B	LYS182
B	GLU218

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146
B	ASP146

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56
B	SER56

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