2PX9
The intrinsic affinity between E2 and the Cys domain of E1 in Ubiquitin-like modifications
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| B | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000795 | cellular_component | synaptonemal complex |
| B | 0001221 | molecular_function | transcription coregulator binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005635 | cellular_component | nuclear envelope |
| B | 0005643 | cellular_component | nuclear pore |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| B | 0007049 | biological_process | cell cycle |
| B | 0007059 | biological_process | chromosome segregation |
| B | 0007084 | biological_process | mitotic nuclear membrane reassembly |
| B | 0008134 | molecular_function | transcription factor binding |
| B | 0016605 | cellular_component | PML body |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016925 | biological_process | protein sumoylation |
| B | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| B | 0019789 | molecular_function | SUMO transferase activity |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0030335 | biological_process | positive regulation of cell migration |
| B | 0032446 | biological_process | protein modification by small protein conjugation |
| B | 0036211 | biological_process | protein modification process |
| B | 0044388 | molecular_function | small protein activating enzyme binding |
| B | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0051168 | biological_process | nuclear export |
| B | 0051301 | biological_process | cell division |
| B | 0061656 | molecular_function | SUMO conjugating enzyme activity |
| B | 0071535 | molecular_function | RING-like zinc finger domain binding |
| B | 0106068 | cellular_component | SUMO ligase complex |
| B | 1903755 | biological_process | positive regulation of SUMO transferase activity |
| B | 1990234 | cellular_component | transferase complex |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Glycyl thioester intermediate |
| Chain | Residue | Details |
| B | CYS93 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | SITE: Interaction with RANBP2 |
| Chain | Residue | Details |
| B | ILE4 | |
| B | VAL25 | |
| B | LEU57 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Substrate binding |
| Chain | Residue | Details |
| B | ASP100 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylserine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| B | SER2 | |
| A | LYS275 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| B | LYS65 | |
| A | LYS371 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by CDK1 => ECO:0000269|PubMed:22509284, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| B | SER71 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate |
| Chain | Residue | Details |
| B | LYS18 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS271 | |
| B | LYS101 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| B | LYS48 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25114211, ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| B | LYS49 |
