2PX5

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Orthorhombic crystal form)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	methyltransferase cap1 activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0004483	molecular_function	methyltransferase cap1 activity
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 902

Chain	Residue
A	ARG28
A	ARG213
A	SER215
A	ASN216
A	HOH719
A	HOH766

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 903

Chain	Residue
A	ARG57
A	ARG84
A	HOH504
A	HOH618
A	ARG37
A	ARG41
A	VAL55

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 904

Chain	Residue
A	ARG63
A	ARG160
A	VAL258
A	ASN259
A	HOH562
A	HOH709
A	HOH761

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 905

Chain	Residue
A	ARG44
A	ARG84
A	GLU111

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 906

Chain	Residue
B	ARG37
B	ARG41
B	ARG84
B	HOH577

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 907

Chain	Residue
B	ARG63
B	GLN159
B	ARG160
B	VAL258
B	ASN259
B	HOH638
B	HOH862

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 908

Chain	Residue
B	ARG44
B	ARG84
B	GLU111
B	HOH769

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 910

Chain	Residue
B	ARG28
B	ARG213
B	SER215
B	ASN216
B	HOH512

---

site_id	AC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 911

Chain	Residue
A	ARG243

---

site_id	BC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SAH A 500

Chain	Residue
A	SER56
A	GLY58
A	GLY81
A	CYS82
A	GLY86
A	TRP87
A	LYS105
A	HIS110
A	GLU111
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	HOH508
A	HOH513
A	HOH520

---

site_id	BC2
Number of Residues	20
Details	BINDING SITE FOR RESIDUE SAH B 500

Chain	Residue
B	SER56
B	GLY58
B	THR59
B	GLY81
B	CYS82
B	GLY83
B	GLY86
B	TRP87
B	LYS105
B	HIS110
B	GLU111
B	VAL130
B	ASP131
B	VAL132
B	PHE133
B	ASP146
B	GLU149
B	HOH509
B	HOH516
B	HOH534

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS182
A	GLU218
B	LYS61
B	ASP146
B	LYS182
B	GLU218

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	SER56
B	SER56
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	ASP131
B	VAL132
B	ILE147
B	TYR220
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	ASP131
A	VAL132
A	ILE147
A	TYR220

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS13
B	PHE24
B	ARG28
B	SER150
B	ARG213
B	SER215
A	ASN17
A	PHE24
A	ARG28
A	SER150
A	ARG213
A	SER215
B	LYS13
B	ASN17

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU16
A	MET19
B	LEU16
B	MET19

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS182
A	GLU218
B	LYS61
B	LYS182
B	GLU218

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146
B	ASP146

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56
B	SER56

---