Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2PKR

Crystal structure of (A+CTE)4 chimeric form of photosyntetic glyceraldehyde-3-phosphate dehydrogenase, complexed with NADP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006006	biological_process	glucose metabolic process
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding
B	0006006	biological_process	glucose metabolic process
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0050661	molecular_function	NADP binding
B	0051287	molecular_function	NAD binding
C	0006006	biological_process	glucose metabolic process
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0050661	molecular_function	NADP binding
C	0051287	molecular_function	NAD binding
D	0006006	biological_process	glucose metabolic process
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0050661	molecular_function	NADP binding
D	0051287	molecular_function	NAD binding
H	0006006	biological_process	glucose metabolic process
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0050661	molecular_function	NADP binding
H	0051287	molecular_function	NAD binding
I	0006006	biological_process	glucose metabolic process
I	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
I	0050661	molecular_function	NADP binding
I	0051287	molecular_function	NAD binding
L	0006006	biological_process	glucose metabolic process
L	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
L	0050661	molecular_function	NADP binding
L	0051287	molecular_function	NAD binding
M	0006006	biological_process	glucose metabolic process
M	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
M	0050661	molecular_function	NADP binding
M	0051287	molecular_function	NAD binding
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0006006	biological_process	glucose metabolic process
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0006006	biological_process	glucose metabolic process
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 O 363

Chain	Residue
O	THR179
O	ASP181
O	ARG195
O	ARG231
O	NDP372

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 O 364

Chain	Residue
O	ALA210
O	SER148
O	THR150
O	THR208
O	GLY209

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 O 371

Chain	Residue
O	PHE251
O	ALA252
O	GLU253

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 R 363

Chain	Residue
R	THR179
R	ASP181
R	ARG195
R	ARG231
R	SO4364
R	NDP377

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 R 364

Chain	Residue
R	SER148
R	CYS149
R	THR150
R	THR208
R	SO4363

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 R 376

Chain	Residue
R	PHE251
R	ALA252
R	VAL299

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 P 363

Chain	Residue
P	THR179
P	ASP181
P	ARG195
P	ARG231
P	SO4364
P	NDP373

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 P 364

Chain	Residue
P	SER148
P	CYS149
P	THR150
P	HIS176
P	THR208
P	GLY209
P	SO4363

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 P 372

Chain	Residue
P	GLU253
P	ASN256
P	ARG260

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 Q 363

Chain	Residue
Q	THR179
Q	ASP181
Q	ARG195
Q	ARG231
Q	SO4364
Q	NDP375

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 Q 364

Chain	Residue
Q	SER148
Q	CYS149
Q	THR150
Q	HIS176
Q	THR208
Q	ARG231
Q	SO4363

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 Q 373

Chain	Residue
O	GLU276
Q	SER41
Q	HIS42
Q	LYS45
Q	TYR46
Q	VAL57

site_id	BC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 Q 374

Chain	Residue
Q	SER62
Q	LYS72

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 363

Chain	Residue
A	THR179
A	ASP181
A	ARG195
A	ARG231
A	NDP376

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 364

Chain	Residue
A	SER148
A	CYS149
A	THR150
A	HIS176
A	THR208
A	GLY209

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 375

Chain	Residue
A	GLU253
A	ASN256
A	ARG260

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 363

Chain	Residue
B	THR179
B	ASP181
B	ARG195
B	ARG231
B	NDP378

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 364

Chain	Residue
B	SER148
B	CYS149
B	THR150
B	HIS176
B	THR208

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 377

Chain	Residue
B	SER41
B	HIS42
B	VAL57
C	GLU276

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 363

Chain	Residue
C	THR179
C	ASP181
C	ARG195
C	NDP365

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 364

Chain	Residue
C	GLY209
C	ALA210
C	SER148
C	THR150
C	THR208

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 363

Chain	Residue
D	THR179
D	ASP181
D	ARG195
D	ARG231
D	NDP365

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 364

Chain	Residue
D	SER148
D	THR150
D	THR208

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 363

Chain	Residue
H	THR179
H	ASP181
H	ARG195
H	ARG231
H	NDP379

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 364

Chain	Residue
H	SER148
H	CYS149
H	THR150
H	HIS176
H	THR208

site_id	CC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 378

Chain	Residue
H	PHE251
H	ALA252
H	GLU253
H	VAL299

site_id	CC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 I 365

Chain	Residue
I	THR179
I	ASP181
I	ARG195
I	ARG231
I	NDP380

site_id	DC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 366

Chain	Residue
I	SER148
I	THR208
I	GLY209
I	ALA210

site_id	DC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 379

Chain	Residue
I	GLU253
I	ASN256
I	ARG260
I	SER294

site_id	DC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 L 367

Chain	Residue
L	THR179
L	ASP181
L	ARG195
L	ARG231
L	NDP369

site_id	DC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 L 368

Chain	Residue
L	SER148
L	THR150
L	HIS176
L	THR208
L	GLY209

site_id	DC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 M 369

Chain	Residue
M	THR179
M	ASP181
M	ARG195
M	ARG231
M	NDP381

site_id	DC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 M 370

Chain	Residue
M	SER148
M	CYS149
M	THR150
M	THR208

site_id	DC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 M 380

Chain	Residue
M	SER62
M	LYS72

site_id	DC8
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NDP O 372

Chain	Residue
O	PHE8
O	GLY9
O	ARG10
O	ILE11
O	ASN31
O	ASP76
O	ARG77
O	GLY95
O	THR96
O	GLY97
O	VAL98
O	PHE99
O	THR119
O	ALA120
O	ASN313
O	GLU314
O	TYR317
O	SO4363
R	ARG183
R	SER188

site_id	DC9
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NDP R 377

Chain	Residue
O	ARG183
O	SER188
R	PHE8
R	GLY9
R	ARG10
R	ILE11
R	ASN31
R	THR33
R	ASP76
R	ARG77
R	GLY95
R	THR96
R	GLY97
R	VAL98
R	PHE99
R	THR119
R	ALA120
R	ASN313
R	GLU314
R	TYR317
R	SO4363

site_id	EC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP P 373

Chain	Residue
P	PHE8
P	GLY9
P	ARG10
P	ILE11
P	ASN31
P	ASP76
P	ARG77
P	GLY95
P	THR96
P	GLY97
P	PHE99
P	THR119
P	ALA120
P	ASN313
P	GLU314
P	TYR317
P	SO4363
Q	ARG183
Q	SER188

site_id	EC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NDP Q 375

Chain	Residue
P	ARG183
P	SER188
Q	GLY7
Q	PHE8
Q	GLY9
Q	ARG10
Q	ILE11
Q	ASN31
Q	THR33
Q	ASP76
Q	ARG77
Q	GLY95
Q	THR96
Q	GLY97
Q	VAL98
Q	PHE99
Q	THR119
Q	ALA120
Q	CYS149
Q	ASN313
Q	TYR317
Q	SO4363

site_id	EC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP A 376

Chain	Residue
A	GLY7
A	PHE8
A	GLY9
A	ARG10
A	ILE11
A	ASN31
A	ASP76
A	ARG77
A	GLY95
A	THR96
A	GLY97
A	PHE99
A	THR119
A	ALA120
A	ASN313
A	GLU314
A	TYR317
A	SO4363
B	SER188

site_id	EC4
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP B 378

Chain	Residue
A	SER188
B	GLY7
B	PHE8
B	GLY9
B	ARG10
B	ILE11
B	ASN31
B	ASP76
B	ARG77
B	GLY95
B	THR96
B	GLY97
B	VAL98
B	PHE99
B	THR119
B	ALA120
B	ASN313
B	TYR317
B	SO4363

site_id	EC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NDP C 365

Chain	Residue
C	PHE8
C	GLY9
C	ARG10
C	ILE11
C	ASN31
C	ASP76
C	ARG77
C	GLY95
C	THR96
C	GLY97
C	VAL98
C	PHE99
C	THR119
C	ALA120
C	ASN313
C	GLU314
C	TYR317
C	SO4363
D	ARG183
D	SER188

site_id	EC6
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP D 365

Chain	Residue
C	ARG183
C	SER188
D	PHE8
D	GLY9
D	ARG10
D	ILE11
D	ASN31
D	ASP76
D	ARG77
D	GLY95
D	THR96
D	GLY97
D	VAL98
D	PHE99
D	THR119
D	ALA120
D	ASN313
D	TYR317
D	SO4363

site_id	EC7
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP H 379

Chain	Residue
H	PHE8
H	GLY9
H	ARG10
H	ILE11
H	ASN31
H	ASP76
H	ARG77
H	GLY95
H	THR96
H	GLY97
H	VAL98
H	PHE99
H	THR119
H	ALA120
H	CYS149
H	ASN313
H	TYR317
H	SO4363
I	SER188

site_id	EC8
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NDP I 380

Chain	Residue
H	ARG183
H	SER188
I	GLY7
I	PHE8
I	GLY9
I	ARG10
I	ILE11
I	ASN31
I	ASP76
I	ARG77
I	GLY95
I	THR96
I	GLY97
I	VAL98
I	PHE99
I	THR119
I	ALA120
I	ASN313
I	TYR317
I	SO4365

site_id	EC9
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NDP L 369

Chain	Residue
L	GLY7
L	PHE8
L	GLY9
L	ARG10
L	ILE11
L	ASN31
L	ASP76
L	ARG77
L	GLY95
L	THR96
L	GLY97
L	VAL98
L	PHE99
L	THR119
L	ALA120
L	CYS149
L	ASN313
L	TYR317
L	SO4367
M	ARG183
M	SER188

site_id	FC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NDP M 381

Chain	Residue
L	SER188
M	GLY7
M	PHE8
M	GLY9
M	ARG10
M	ILE11
M	ASN31
M	ASP76
M	ARG77
M	GLY95
M	THR96
M	GLY97
M	VAL98
M	PHE99
M	THR119
M	ALA120
M	ASN313
M	TYR317
M	SO4369

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA147-LEU154

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
O	CYS149
I	CYS149
L	CYS149
M	CYS149
R	CYS149
P	CYS149
Q	CYS149
A	CYS149
B	CYS149
C	CYS149
D	CYS149
H	CYS149

site_id	SWS_FT_FI2
Number of Residues	48
Details	BINDING: BINDING => ECO:0000269\|PubMed:11846565, ECO:0000269\|PubMed:12705826, ECO:0000269\|PubMed:15236965, ECO:0000269\|PubMed:17573533

Chain	Residue	Details
O	ARG10
P	ASP32
P	ARG77
P	ASN313
Q	ARG10
Q	ASP32
Q	ARG77
Q	ASN313
A	ARG10
A	ASP32
A	ARG77
O	ASP32
A	ASN313
B	ARG10
B	ASP32
B	ARG77
B	ASN313
C	ARG10
C	ASP32
C	ARG77
C	ASN313
D	ARG10
O	ARG77
D	ASP32
D	ARG77
D	ASN313
H	ARG10
H	ASP32
H	ARG77
H	ASN313
I	ARG10
I	ASP32
I	ARG77
O	ASN313
I	ASN313
L	ARG10
L	ASP32
L	ARG77
L	ASN313
M	ARG10
M	ASP32
M	ARG77
M	ASN313
R	ARG10
R	ASP32
R	ARG77
R	ASN313
P	ARG10

site_id	SWS_FT_FI3
Number of Residues	60
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
O	SER148
R	ARG231
P	SER148
P	THR179
P	ARG195
P	THR208
P	ARG231
Q	SER148
Q	THR179
Q	ARG195
Q	THR208
O	THR179
Q	ARG231
A	SER148
A	THR179
A	ARG195
A	THR208
A	ARG231
B	SER148
B	THR179
B	ARG195
B	THR208
O	ARG195
B	ARG231
C	SER148
C	THR179
C	ARG195
C	THR208
C	ARG231
D	SER148
D	THR179
D	ARG195
D	THR208
O	THR208
D	ARG231
H	SER148
H	THR179
H	ARG195
H	THR208
H	ARG231
I	SER148
I	THR179
I	ARG195
I	THR208
O	ARG231
I	ARG231
L	SER148
L	THR179
L	ARG195
L	THR208
L	ARG231
M	SER148
M	THR179
M	ARG195
M	THR208
R	SER148
M	ARG231
R	THR179
R	ARG195
R	THR208

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: Activates thiol group during catalysis => ECO:0000250

Chain	Residue	Details
O	HIS176
I	HIS176
L	HIS176
M	HIS176
R	HIS176
P	HIS176
Q	HIS176
A	HIS176
B	HIS176
C	HIS176
D	HIS176
H	HIS176

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
O	CYS149
O	HIS176

site_id	CSA10
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
I	CYS149
I	HIS176

site_id	CSA11
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
L	CYS149
L	HIS176

site_id	CSA12
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
M	CYS149
M	HIS176

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
R	CYS149
R	HIS176

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
P	CYS149
P	HIS176

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
Q	CYS149
Q	HIS176

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
A	CYS149
A	HIS176

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
B	CYS149
B	HIS176

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
C	CYS149
C	HIS176

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
D	CYS149
D	HIS176

site_id	CSA9
Number of Residues	2
Details	Annotated By Reference To The Literature 1szj

Chain	Residue	Details
H	CYS149
H	HIS176

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)