2OWO

Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003911	molecular_function	DNA ligase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006259	biological_process	DNA metabolic process
A	0006260	biological_process	DNA replication
A	0006266	biological_process	DNA ligation
A	0006281	biological_process	DNA repair
A	0006288	biological_process	base-excision repair, DNA ligation
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
A	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 672

Chain	Residue
A	CYS408
A	CYS411
A	CYS426
A	CYS432

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 673

Chain	Residue
A	ARG446
A	ARG447
A	HOH922
C	HOH42
D	DC40

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 674

Chain	Residue
A	ARG74
A	ARG510
A	HOH718
A	HOH759

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 675

Chain	Residue
A	ASN95
A	GLN99
A	SER262
A	ALA263
A	HOH799

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 676

Chain	Residue
A	HIS559
A	ASN562

---

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE AMP D 53

Chain	Residue
A	SER81
A	LEU82
A	GLU113
A	LYS115
A	ALA120
A	ARG136
A	GLU173
A	TYR225
A	VAL288
A	LYS290
A	HOH752
C	HOH137
D	DC40

---

Functional Information from PROSITE/UniProt

site_id	PS01055
Number of Residues	30
Details	DNA_LIGASE_N1 NAD-dependent DNA ligase signature 1. KLDGLAvsilYenGvLvsaaTRGDGttGED

Chain	Residue	Details
A	LYS115-ASP144

---

site_id	PS01056
Number of Residues	16
Details	DNA_LIGASE_N2 NAD-dependent DNA ligase signature 2. VGRTGaITpVarLePV

Chain	Residue	Details
A	VAL331-VAL346

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: N6-AMP-lysine intermediate => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000269|PubMed:11781321

Chain	Residue	Details
A	LYS115

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588

Chain	Residue	Details
A	ASP32
A	GLU173
A	LYS314

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000305|PubMed:17466627

Chain	Residue	Details
A	SER81
A	GLU113
A	ARG136
A	LYS290

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000269|PubMed:17466627

Chain	Residue	Details
A	CYS408
A	CYS411
A	CYS426
A	CYS432

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Interaction with target DNA => ECO:0000305|PubMed:17466627

Chain	Residue	Details
A	ARG487
A	SER492

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1b04

Chain	Residue	Details
A	LYS115

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1b04

Chain	Residue	Details
A	ARG200
A	ASP117
A	LYS314
A	LYS115

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