2OQL

Structure of Phosphotriesterase mutant H254Q/H257F

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004063	molecular_function	aryldialkylphosphatase activity
A	0005886	cellular_component	plasma membrane
A	0008270	molecular_function	zinc ion binding
A	0009056	biological_process	catabolic process
A	0016787	molecular_function	hydrolase activity
A	0016788	molecular_function	hydrolase activity, acting on ester bonds
A	0046872	molecular_function	metal ion binding
B	0004063	molecular_function	aryldialkylphosphatase activity
B	0005886	cellular_component	plasma membrane
B	0008270	molecular_function	zinc ion binding
B	0009056	biological_process	catabolic process
B	0016787	molecular_function	hydrolase activity
B	0016788	molecular_function	hydrolase activity, acting on ester bonds
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 401

Chain	Residue
A	HIS55
A	HIS57
A	KCX169
A	ASP301
A	GOL802
A	HOH856

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 402

Chain	Residue
A	HOH856
A	HOH988
A	HOH1098
A	KCX169
A	HIS201
A	HIS230

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 401

Chain	Residue
B	HIS55
B	HIS57
B	KCX169
B	ASP301
B	GOL803
B	HOH1041

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 402

Chain	Residue
B	KCX169
B	HIS201
B	HIS230
B	HOH1027
B	HOH1038
B	HOH1041

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE BTB A 701

Chain	Residue
A	PHE72
A	PHE73
A	THR311
A	ASP315
A	HOH826
A	HOH849
A	HOH934
A	HOH1074
A	HOH1086
A	HOH1139

---

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE BTB B 701

Chain	Residue
B	PHE72
B	PHE73
B	THR311
B	ASP315
B	HOH817
B	HOH913
B	HOH976
B	HOH1074
B	HOH1096
B	HOH1116

---

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 802

Chain	Residue
A	HIS57
A	TRP131
A	KCX169
A	ASP301
A	ZN401
A	HOH856
A	HOH924
A	HOH980
A	HOH1098
A	HOH1104

---

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL B 801

Chain	Residue
B	ALA63
B	GLY64
B	ARG67
B	ARG108
B	ASP109
B	GLU159
B	HOH818
B	HOH851
B	HOH891

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL B 803

Chain	Residue
B	HIS57
B	KCX169
B	ASP301
B	ZN401
B	HOH941
B	HOH1027
B	HOH1038
B	HOH1041

---

Functional Information from PROSITE/UniProt

site_id	PS01322
Number of Residues	9
Details	PHOSPHOTRIESTERASE_1 Phosphotriesterase family signature 1. GfTLtHEHI

Chain	Residue	Details
A	GLY50-ILE58

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7794910","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DPM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EYW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EZ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1HZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4M","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OB3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"description":"via carbamate group","evidences":[{"source":"PubMed","id":"7794910","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DPM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EYW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EZ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1HZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4M","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OB3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00679","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7794910","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1DPM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EYW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1EZ2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1HZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4M","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O4Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OB3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OQL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1ez2

Chain	Residue	Details
A	GLN254
A	ASP233
A	ASP301

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1ez2

Chain	Residue	Details
B	GLN254
B	ASP233
B	ASP301

---

site_id	MCSA1
Number of Residues	8
Details	M-CSA 159

Chain	Residue	Details
A	HIS55	metal ligand
A	HIS57	metal ligand
A	KCX169	metal ligand
A	SER205	metal ligand
A	THR234	metal ligand
A	LEU237	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	SER258	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
A	GLY305	hydrogen bond acceptor, hydrogen bond donor, metal ligand, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	8
Details	M-CSA 159

Chain	Residue	Details
B	HIS55	metal ligand
B	HIS57	metal ligand
B	KCX169	metal ligand
B	SER205	metal ligand
B	THR234	metal ligand
B	LEU237	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	SER258	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
B	GLY305	hydrogen bond acceptor, hydrogen bond donor, metal ligand, proton acceptor, proton donor

---