2NUG

Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0003725	molecular_function	double-stranded RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004525	molecular_function	ribonuclease III activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006364	biological_process	rRNA processing
A	0006396	biological_process	RNA processing
A	0006397	biological_process	mRNA processing
A	0008033	biological_process	tRNA processing
A	0010468	biological_process	regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0003725	molecular_function	double-stranded RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004525	molecular_function	ribonuclease III activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006364	biological_process	rRNA processing
B	0006396	biological_process	RNA processing
B	0006397	biological_process	mRNA processing
B	0008033	biological_process	tRNA processing
B	0010468	biological_process	regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	ASP44
A	GLU110
A	HOH518
A	HOH519
D	G12
D	HOH517
E	A17

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	MG503
A	HOH519
A	HOH520
A	HOH531
E	A17
E	HOH506
A	GLU40
A	GLU110

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 503

Chain	Residue
A	ASP107
A	MG502
A	HOH521
A	HOH522
A	HOH523
E	HOH506
E	HOH507

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 504

Chain	Residue
A	GLU37
A	GLU40
A	HOH524
A	HOH525
B	HOH519
E	G18
E	HOH508

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG E 505

Chain	Residue
E	A17
E	G18
E	HOH509
E	HOH510
E	HOH511
E	HOH512

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 506

Chain	Residue
A	HOH526
A	HOH527
A	HOH528
A	HOH529
C	HOH520
C	HOH521

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 507

Chain	Residue
B	ASP44
B	GLU110
B	HOH520
C	G12
C	HOH522
C	HOH523
F	A17

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 508

Chain	Residue
B	GLU40
B	GLU110
B	HOH521
B	HOH530
C	HOH523
F	A17
F	HOH514

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 509

Chain	Residue
B	ASP107
B	HOH521
B	HOH522
B	HOH523
B	HOH524
B	HOH530
F	HOH515

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 510

Chain	Residue
A	HOH530
B	GLU37
B	GLU40
B	HOH525
B	HOH526
F	HOH516

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG F 511

Chain	Residue
F	A17
F	G18
F	HOH517
F	HOH518
F	HOH519
F	HOH520

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 512

Chain	Residue
B	HOH527
B	HOH528
B	HOH529
D	HOH518
D	HOH519
D	HOH563

---

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG F 513

Chain	Residue
E	HOH522
F	HOH542
F	HOH543
F	HOH544
F	HOH545
F	HOH552

---

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 514

Chain	Residue
D	HOH529
D	HOH533
D	HOH550
D	HOH551
D	HOH552

---

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG C 515

Chain	Residue
C	HOH535
C	HOH538
C	HOH559
C	HOH563

---

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG D 516

Chain	Residue
E	HOH513
E	HOH526
D	HOH522
D	HOH531
D	HOH535
D	HOH562

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 517

Chain	Residue
A	GLY12
A	HOH534
A	HOH620
A	HOH632
B	ARG194

---

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG B 518

Chain	Residue
B	HOH538
B	HOH561
B	HOH657

---

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 519

Chain	Residue
C	HOH526
C	HOH530
C	HOH540
C	HOH546
C	HOH562
F	HOH551

---

Functional Information from PROSITE/UniProt

site_id	PS00517
Number of Residues	9
Details	RNASE_3_1 Ribonuclease III family signature. ETLEFLGDA

Chain	Residue	Details
A	GLU37-ALA45

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	234
Details	Domain: {"description":"RNase III"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15016361","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18047582","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11738048","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1JFZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1RC5","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	68
Details	Domain: {"description":"DRBM"}

Chain

Residue

Details

---