2NPJ
An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0008519 | molecular_function | ammonium channel activity |
| A | 0015670 | biological_process | carbon dioxide transport |
| A | 0016020 | cellular_component | membrane |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0072488 | biological_process | ammonium transmembrane transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 601 |
| Chain | Residue |
| A | VAL147 |
| A | ALA158 |
| A | LEU159 |
| A | ASP160 |
| A | TYR278 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE IMD A 701 |
| Chain | Residue |
| A | SER219 |
| A | HOH827 |
| A | GLN104 |
| A | PHE107 |
| A | TRP148 |
| A | PHE161 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE IMD A 801 |
| Chain | Residue |
| A | HIS100 |
| A | PHE103 |
| A | GLN104 |
| A | SER219 |
| A | GLY221 |
| A | HOH845 |
| A | HOH888 |
Functional Information from PROSITE/UniProt
| site_id | PS01219 |
| Number of Residues | 26 |
| Details | AMMONIUM_TRANSP Ammonium transporters signature. DFAGGtvVhinAAiaGLvgaYLiGkR |
| Chain | Residue | Details |
| A | ASP160-ARG185 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 236 |
| Details | Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 62 |
| Details | Topological domain: {"description":"Periplasmic","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for the deprotonation of the ammonium cation","evidences":[{"source":"PubMed","id":"19278252","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Twin-His motif. Important for optimum substrate conductance","evidences":[{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"23667517","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"32662768","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for optimum substrate conductance","evidences":[{"source":"PubMed","id":"18362341","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
