2NP6

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0006304	biological_process	DNA modification
A	0008168	molecular_function	methyltransferase activity
A	0009007	molecular_function	site-specific DNA-methyltransferase (adenine-specific) activity
A	0009307	biological_process	DNA restriction-modification system
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
D	0003676	molecular_function	nucleic acid binding
D	0003677	molecular_function	DNA binding
D	0006304	biological_process	DNA modification
D	0008168	molecular_function	methyltransferase activity
D	0009007	molecular_function	site-specific DNA-methyltransferase (adenine-specific) activity
D	0009307	biological_process	DNA restriction-modification system
D	0016740	molecular_function	transferase activity
D	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE NEA A 601

Chain	Residue
A	ARG20
A	ASP89
A	PHE90
A	ASN105
A	PRO107
A	PHE146
A	HOH724
A	HOH788
A	HOH850
A	HOH907
A	VAL21
A	ALA47
A	ALA49
A	GLU71
A	ILE72
A	ASP73
A	ALA76
A	ALA88

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE NEA D 602

Chain	Residue
D	VAL21
D	ALA47
D	GLU71
D	ILE72
D	ALA88
D	ASP89
D	PHE90
D	ASN105
D	PRO107
D	PHE146
D	GOL701
D	GOL702
D	HOH786

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 701

Chain	Residue
D	VAL121
D	TYR129
D	LEU142
D	NEA602

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL D 702

Chain	Residue
D	VAL21
D	ASN105
D	PRO106
D	PRO107
D	TYR108
D	LYS199
D	NEA602

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE IPA A 703

Chain	Residue
A	ILE242
A	ARG244
A	GLU246
A	ARG402
A	HOH869

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE IPA E 704

Chain	Residue
D	ARG296
E	DG8
F	DA14
F	DT15

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE IPA B 705

Chain	Residue
A	ARG296
B	DG8
B	DT9
C	DT15
C	HOH26

---

Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. ILGNPPY

Chain	Residue	Details
A	ILE102-TYR108

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	BINDING: BINDING => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	THR23
D	PRO107
A	GLU45
A	GLU71
A	ASP89
A	PRO107
D	THR23
D	GLU45
D	GLU71
D	ASP89

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Important for catalytic activity => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	ASN105
A	TYR108
D	ASN105
D	TYR108

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Important for catalytic activity; via amide nitrogen => ECO:0007744|PDB:2ADM

Chain	Residue	Details
A	PRO106
D	PRO106

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 2adm

Chain	Residue	Details
A	PRO106
A	ASN105
A	TYR108

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 2adm

Chain	Residue	Details
D	PRO106
D	ASN105
D	TYR108

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 46

Chain

Residue

Details

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 46

Chain

Residue

Details

---