2NAP

DISSIMILATORY NITRATE REDUCTASE (NAP) FROM DESULFOVIBRIO DESULFURICANS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005506	molecular_function	iron ion binding
A	0006777	biological_process	Mo-molybdopterin cofactor biosynthetic process
A	0008940	molecular_function	nitrate reductase activity
A	0009055	molecular_function	electron transfer activity
A	0009325	cellular_component	nitrate reductase complex
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0030151	molecular_function	molybdenum ion binding
A	0042128	biological_process	nitrate assimilation
A	0042597	cellular_component	periplasmic space
A	0043546	molecular_function	molybdopterin cofactor binding
A	0045333	biological_process	cellular respiration
A	0046872	molecular_function	metal ion binding
A	0050140	molecular_function	nitrate reductase (cytochrome) activity
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051539	molecular_function	4 iron, 4 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SF4 A 800

Chain	Residue
A	CYS47
A	VAL183
A	CYS13
A	TYR15
A	CYS16
A	GLY19
A	CYS20

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MO A 810

Chain	Residue
A	CYS140
A	MGD811
A	MGD812
A	HOH813

---

site_id	AC3
Number of Residues	33
Details	BINDING SITE FOR RESIDUE MGD A 811

Chain	Residue
A	ARG14
A	GLN111
A	ASN136
A	CYS140
A	GLN312
A	GLU416
A	THR417
A	ASN418
A	THR422
A	ILE443
A	GLU444
A	ALA445
A	PHE446
A	ALA449
A	PRO461
A	ALA462
A	PHE463
A	SER615
A	ARG617
A	TRP622
A	HIS623
A	THR624
A	THR626
A	MET627
A	PHE689
A	ASN697
A	TYR713
A	LYS714
A	MO810
A	MGD812
A	HOH1181
A	HOH1249
A	HOH1507

---

site_id	AC4
Number of Residues	36
Details	BINDING SITE FOR RESIDUE MGD A 812

Chain	Residue
A	CYS16
A	LYS49
A	CYS140
A	ILE173
A	GLY174
A	SER175
A	ASN176
A	GLU179
A	ALA180
A	ASP204
A	PRO205
A	ARG206
A	PHE220
A	PRO222
A	GLY223
A	ASP225
A	CYS307
A	MET308
A	GLY309
A	ARG313
A	GLY345
A	THR614
A	MET616
A	ARG617
A	VAL618
A	ILE619
A	HIS621
A	TRP622
A	HIS623
A	LYS714
A	MO810
A	MGD811
A	HOH1074
A	HOH1099
A	HOH1150
A	HOH1251

---

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE MES A 2300

Chain	Residue
A	ALA142
A	VAL145
A	GLY146
A	VAL149
A	THR150
A	HIS361
A	VAL707
A	SER708
A	HOH1096
A	HOH1276
A	HOH1347
A	HOH1456
A	HOH1542

---

site_id	FS4
Number of Residues	1
Details	4 IRON - 4 SULFUR CLUSTER

Chain	Residue
A	SF4800

---

site_id	MGD
Number of Residues	3
Details	MOLYBDOPTERIN GUANINE DINUCLEOTIDE COFACTOR

Chain	Residue
A	MO810
A	MGD811
A	MGD812

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	55
Details	Domain: {"description":"4Fe-4S Mo/W bis-MGD-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01630","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01630","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10368307","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2JIM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NAP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V3V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V45","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01630","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10368307","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2JIM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NAP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V3V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V45","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10368307","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2JIM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NAP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V3V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V45","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01630","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10368307","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2JIM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NAP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V3V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V45","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2JIQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01630","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18327621","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2JIQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JIR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1tmo

Chain	Residue	Details
A	CYS140

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1tmo

Chain	Residue	Details
A	ARG138

---