Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2J9D

Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006808	biological_process	regulation of nitrogen utilization
A	0030234	molecular_function	enzyme regulator activity
B	0000166	molecular_function	nucleotide binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006808	biological_process	regulation of nitrogen utilization
B	0030234	molecular_function	enzyme regulator activity
C	0000166	molecular_function	nucleotide binding
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006808	biological_process	regulation of nitrogen utilization
C	0030234	molecular_function	enzyme regulator activity
D	0000166	molecular_function	nucleotide binding
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006808	biological_process	regulation of nitrogen utilization
D	0030234	molecular_function	enzyme regulator activity
E	0000166	molecular_function	nucleotide binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006808	biological_process	regulation of nitrogen utilization
E	0030234	molecular_function	enzyme regulator activity
F	0000166	molecular_function	nucleotide binding
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006808	biological_process	regulation of nitrogen utilization
F	0030234	molecular_function	enzyme regulator activity
G	0000166	molecular_function	nucleotide binding
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006808	biological_process	regulation of nitrogen utilization
G	0030234	molecular_function	enzyme regulator activity
H	0000166	molecular_function	nucleotide binding
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006808	biological_process	regulation of nitrogen utilization
H	0030234	molecular_function	enzyme regulator activity
I	0000166	molecular_function	nucleotide binding
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006808	biological_process	regulation of nitrogen utilization
I	0030234	molecular_function	enzyme regulator activity
J	0000166	molecular_function	nucleotide binding
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006808	biological_process	regulation of nitrogen utilization
J	0030234	molecular_function	enzyme regulator activity
K	0000166	molecular_function	nucleotide binding
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006808	biological_process	regulation of nitrogen utilization
K	0030234	molecular_function	enzyme regulator activity
L	0000166	molecular_function	nucleotide binding
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006808	biological_process	regulation of nitrogen utilization
L	0030234	molecular_function	enzyme regulator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT A1116

Chain	Residue
A	LYS3
A	GLU5
B	LYS3
B	GLU5
C	LYS3
C	GLU5
C	ILE94

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ACT A1117

Chain	Residue
A	GLY87
A	ASP88
A	HOH2043
A	HOH2057
C	ARG101
C	ARG103
A	ASN85
A	PRO86

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE ACT E1116

Chain	Residue
D	LYS3
D	GLU5
D	ILE94
E	LYS3
E	GLU5
F	LYS3
F	GLU5
F	ILE94

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT H1113

Chain	Residue
G	LYS3
G	GLU5
G	ILE94
H	LYS3
H	GLU5
I	LYS3
I	GLU5

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL J1114

Chain	Residue
J	LYS60

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT J1116

Chain	Residue
J	LYS3
J	GLU5
K	LYS3
K	GLU5
L	LYS3
L	GLU5

site_id	AC7
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ADP B1115

Chain	Residue
B	GLY27
B	MET28
B	THR29
B	GLU62
B	LEU63
B	VAL64
B	ARG101
B	ARG103
B	HOH2077
B	HOH2078
C	ILE7
C	GLY35
C	ARG36
C	GLY37
C	VAL38
C	LYS58
C	GLY87
C	ASP88
C	GLY89
C	LYS90

site_id	AC8
Number of Residues	16
Details	BINDING SITE FOR RESIDUE AMP E1115

Chain	Residue
E	GLY27
E	MET28
E	THR29
E	GLU62
E	LEU63
E	VAL64
E	ARG101
E	GLU114
F	ILE7
F	GLY35
F	VAL38
F	LYS58
F	GLY87
F	ASP88
F	GLY89
F	LYS90

site_id	AC9
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ADP I1115

Chain	Residue
H	GLY27
H	THR29
H	GLU62
H	LEU63
H	VAL64
H	ARG101
H	ARG103
I	ILE7
I	GLY35
I	ARG36
I	LYS58
I	ASN85
I	PRO86
I	GLY87
I	ASP88
I	GLY89
I	LYS90
I	PHE92
I	HOH2040
I	HOH2041

site_id	BC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ADP J1115

Chain	Residue
J	PRO86
J	GLY87
J	ASP88
J	GLY89
J	LYS90
J	HOH2043
J	HOH2044
L	GLY27
L	THR29
L	GLU62
L	LEU63
L	VAL64
L	ARG101
L	ARG103
J	ILE7
J	GLY35
J	ARG36
J	GLY37
J	VAL38
J	LYS58

site_id	BC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ADP L1114

Chain	Residue
K	GLY27
K	MET28
K	THR29
K	GLU62
K	LEU63
K	VAL64
K	ARG101
K	ARG103
K	GLU114
L	ILE7
L	GLY35
L	ARG36
L	GLY37
L	VAL38
L	GLN39
L	LYS58
L	GLY87
L	ASP88
L	GLY89
L	LYS90
L	HOH2072
L	HOH2073

Functional Information from PROSITE/UniProt

site_id	PS00638
Number of Residues	14
Details	PII_GLNB_CTER P-II protein C-terminal region signature. TgnpGDGKIFVipV

Chain	Residue	Details
E	THR83-VAL96
A	THR83-VAL96

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	107
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17203075","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2J9C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J9E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17203075","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2J9D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17203075","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)