Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2J2F

The T199D Mutant of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0006631	biological_process	fatty acid metabolic process
A	0006633	biological_process	fatty acid biosynthetic process
A	0009507	cellular_component	chloroplast
A	0016491	molecular_function	oxidoreductase activity
A	0045300	molecular_function	stearoyl-[ACP] desaturase activity
A	0046872	molecular_function	metal ion binding
A	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity
B	0005515	molecular_function	protein binding
B	0006631	biological_process	fatty acid metabolic process
B	0006633	biological_process	fatty acid biosynthetic process
B	0009507	cellular_component	chloroplast
B	0016491	molecular_function	oxidoreductase activity
B	0045300	molecular_function	stearoyl-[ACP] desaturase activity
B	0046872	molecular_function	metal ion binding
B	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity
C	0005515	molecular_function	protein binding
C	0006631	biological_process	fatty acid metabolic process
C	0006633	biological_process	fatty acid biosynthetic process
C	0009507	cellular_component	chloroplast
C	0016491	molecular_function	oxidoreductase activity
C	0045300	molecular_function	stearoyl-[ACP] desaturase activity
C	0046872	molecular_function	metal ion binding
C	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity
D	0005515	molecular_function	protein binding
D	0006631	biological_process	fatty acid metabolic process
D	0006633	biological_process	fatty acid biosynthetic process
D	0009507	cellular_component	chloroplast
D	0016491	molecular_function	oxidoreductase activity
D	0045300	molecular_function	stearoyl-[ACP] desaturase activity
D	0046872	molecular_function	metal ion binding
D	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity
E	0005515	molecular_function	protein binding
E	0006631	biological_process	fatty acid metabolic process
E	0006633	biological_process	fatty acid biosynthetic process
E	0009507	cellular_component	chloroplast
E	0016491	molecular_function	oxidoreductase activity
E	0045300	molecular_function	stearoyl-[ACP] desaturase activity
E	0046872	molecular_function	metal ion binding
E	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity
F	0005515	molecular_function	protein binding
F	0006631	biological_process	fatty acid metabolic process
F	0006633	biological_process	fatty acid biosynthetic process
F	0009507	cellular_component	chloroplast
F	0016491	molecular_function	oxidoreductase activity
F	0045300	molecular_function	stearoyl-[ACP] desaturase activity
F	0046872	molecular_function	metal ion binding
F	0102786	molecular_function	obsolete stearoyl-[acp] desaturase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE A 370

Chain	Residue
A	GLU105
A	GLU143
A	HIS146
A	GLU229
A	HOH2010

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE A 371

Chain	Residue
A	HIS232
A	HOH2016
A	TRP139
A	GLU143
A	GLU196
A	GLU229

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE B 370

Chain	Residue
B	GLU105
B	GLU143
B	HIS146
B	GLU229
B	HOH2019

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE B 371

Chain	Residue
B	TRP139
B	GLU143
B	GLU196
B	GLU229
B	HIS232
B	HOH2032

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE C 370

Chain	Residue
C	GLU105
C	GLU143
C	HIS146
C	GLU229
C	HOH2012

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE C 371

Chain	Residue
C	GLU143
C	GLU196
C	GLU229
C	HIS232
C	HOH2023

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE D 370

Chain	Residue
D	GLU105
D	GLU143
D	HIS146
D	GLU229

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE D 371

Chain	Residue
D	TRP139
D	GLU143
D	GLU196
D	GLU229
D	HIS232
D	HOH2027

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE E 370

Chain	Residue
E	GLU105
E	GLU143
E	HIS146
E	GLU229

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE E 371

Chain	Residue
E	GLU143
E	GLU196
E	GLU229
E	HIS232
E	HOH2017

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FE F 370

Chain	Residue
F	GLU105
F	GLU143
F	HIS146
F	GLU229
F	HOH2016

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE F 371

Chain	Residue
F	TRP139
F	GLU143
F	GLU196
F	GLU229
F	HIS232
F	HOH2017

Functional Information from PROSITE/UniProt

site_id	PS00574
Number of Residues	20
Details	FATTY_ACID_DESATUR_2 Fatty acid desaturases family 2 signature. SAvaqRLgvytakDYadILE

Chain	Residue	Details
A	SER283-GLU302

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	36
Details	BINDING: BINDING => ECO:0000269\|PubMed:12704186, ECO:0000269\|PubMed:17088542, ECO:0000269\|PubMed:8861937

Chain	Residue	Details
A	GLU105
B	GLU196
B	GLU229
B	HIS232
C	GLU105
C	GLU143
C	HIS146
C	GLU196
C	GLU229
C	HIS232
D	GLU105
A	GLU143
D	GLU143
D	HIS146
D	GLU196
D	GLU229
D	HIS232
E	GLU105
E	GLU143
E	HIS146
E	GLU196
E	GLU229
A	HIS146
E	HIS232
F	GLU105
F	GLU143
F	HIS146
F	GLU196
F	GLU229
F	HIS232
A	GLU196
A	GLU229
A	HIS232
B	GLU105
B	GLU143
B	HIS146

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
A	ASP199
A	ASP228
A	TRP62
A	HIS146

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
B	ASP199
B	ASP228
B	TRP62
B	HIS146

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
C	ASP199
C	ASP228
C	TRP62
C	HIS146

site_id	CSA4
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
D	ASP199
D	ASP228
D	TRP62
D	HIS146

site_id	CSA5
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
E	ASP199
E	ASP228
E	TRP62
E	HIS146

site_id	CSA6
Number of Residues	4
Details	Annotated By Reference To The Literature 1afr

Chain	Residue	Details
F	ASP199
F	ASP228
F	TRP62
F	HIS146

site_id	MCSA1
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
A	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
A	GLU105	metal ligand
A	GLU143	metal ligand
A	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
A	GLU196	metal ligand
A	ASP199	electrostatic stabiliser, hydrogen bond donor
A	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
A	GLU229	metal ligand
A	HIS232	metal ligand

site_id	MCSA2
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
B	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
B	GLU105	metal ligand
B	GLU143	metal ligand
B	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
B	GLU196	metal ligand
B	ASP199	electrostatic stabiliser, hydrogen bond donor
B	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
B	GLU229	metal ligand
B	HIS232	metal ligand

site_id	MCSA3
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
C	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
C	GLU105	metal ligand
C	GLU143	metal ligand
C	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
C	GLU196	metal ligand
C	ASP199	electrostatic stabiliser, hydrogen bond donor
C	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
C	GLU229	metal ligand
C	HIS232	metal ligand

site_id	MCSA4
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
D	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
D	GLU105	metal ligand
D	GLU143	metal ligand
D	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
D	GLU196	metal ligand
D	ASP199	electrostatic stabiliser, hydrogen bond donor
D	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
D	GLU229	metal ligand
D	HIS232	metal ligand

site_id	MCSA5
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
E	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
E	GLU105	metal ligand
E	GLU143	metal ligand
E	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
E	GLU196	metal ligand
E	ASP199	electrostatic stabiliser, hydrogen bond donor
E	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
E	GLU229	metal ligand
E	HIS232	metal ligand

site_id	MCSA6
Number of Residues	9
Details	M-CSA 136

Chain	Residue	Details
F	TRP62	hydrogen bond donor, single electron acceptor, single electron donor, single electron relay
F	GLU105	metal ligand
F	GLU143	metal ligand
F	HIS146	hydrogen bond donor, metal ligand, single electron acceptor, single electron donor, single electron relay
F	GLU196	metal ligand
F	ASP199	electrostatic stabiliser, hydrogen bond donor
F	ASP228	hydrogen bond acceptor, single electron acceptor, single electron donor, single electron relay
F	GLU229	metal ligand
F	HIS232	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)