2IWM
precursor mutant Cys1Ser of Penicillin V Acylase from Bacillus sphaericus
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008953 | molecular_function | penicillin amidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046677 | biological_process | response to antibiotic |
| B | 0008953 | molecular_function | penicillin amidase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046677 | biological_process | response to antibiotic |
| C | 0008953 | molecular_function | penicillin amidase activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0046677 | biological_process | response to antibiotic |
| D | 0008953 | molecular_function | penicillin amidase activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0046677 | biological_process | response to antibiotic |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Propeptide: {"description":"Removed in mature form","featureId":"PRO_0000045278","evidences":[{"source":"PubMed","id":"10331865","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16508111","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"10331865","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16508111","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 241 |
| Chain | Residue | Details |
| A | SER4 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | TYR85 | electrostatic stabiliser, hydrogen bond donor |
| A | ASN178 | electrostatic stabiliser, hydrogen bond donor |
| A | ARG231 | activator, hydrogen bond donor, increase acidity, increase basicity, repulsive charge-charge interaction |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 241 |
| Chain | Residue | Details |
| B | SER4 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | TYR85 | electrostatic stabiliser, hydrogen bond donor |
| B | ASN178 | electrostatic stabiliser, hydrogen bond donor |
| B | ARG231 | activator, hydrogen bond donor, increase acidity, increase basicity, repulsive charge-charge interaction |
| site_id | MCSA3 |
| Number of Residues | 4 |
| Details | M-CSA 241 |
| Chain | Residue | Details |
| C | SER4 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| C | TYR85 | electrostatic stabiliser, hydrogen bond donor |
| C | ASN178 | electrostatic stabiliser, hydrogen bond donor |
| C | ARG231 | activator, hydrogen bond donor, increase acidity, increase basicity, repulsive charge-charge interaction |
| site_id | MCSA4 |
| Number of Residues | 4 |
| Details | M-CSA 241 |
| Chain | Residue | Details |
| D | SER4 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| D | TYR85 | electrostatic stabiliser, hydrogen bond donor |
| D | ASN178 | electrostatic stabiliser, hydrogen bond donor |
| D | ARG231 | activator, hydrogen bond donor, increase acidity, increase basicity, repulsive charge-charge interaction |
