Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006749 | biological_process | glutathione metabolic process |
| A | 0008216 | biological_process | spermidine metabolic process |
| A | 0008884 | molecular_function | glutathionylspermidine amidase activity |
| A | 0008885 | molecular_function | glutathionylspermidine synthase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016874 | molecular_function | ligase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005829 | cellular_component | cytosol |
| B | 0006749 | biological_process | glutathione metabolic process |
| B | 0008216 | biological_process | spermidine metabolic process |
| B | 0008884 | molecular_function | glutathionylspermidine amidase activity |
| B | 0008885 | molecular_function | glutathionylspermidine synthase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016874 | molecular_function | ligase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 5001 |
| Chain | Residue |
| A | GLU330 |
| A | ASN332 |
| A | ADP3001 |
| A | GGA6001 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 5002 |
| Chain | Residue |
| A | ASP318 |
| A | GLU330 |
| A | ADP3001 |
| A | HOH4190 |
| A | GGA6001 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 5003 |
| Chain | Residue |
| B | GLU330 |
| B | ASN332 |
| B | ADP3002 |
| B | HOH4024 |
| B | GGA6002 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 5004 |
| Chain | Residue |
| B | ASP318 |
| B | GLU330 |
| B | ADP3002 |
| B | HOH4293 |
| B | GGA6002 |
| site_id | AC5 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE GGA A 6001 |
| Chain | Residue |
| A | ASN241 |
| A | ARG316 |
| A | ASP318 |
| A | GLU330 |
| A | ASN332 |
| A | SER335 |
| A | ALA336 |
| A | SER337 |
| A | ASP387 |
| A | GLU391 |
| A | GLU392 |
| A | THR441 |
| A | TRP442 |
| A | ALA443 |
| A | THR446 |
| A | ARG538 |
| A | CYS539 |
| A | ARG598 |
| A | LYS607 |
| A | ASP610 |
| A | ADP3001 |
| A | HOH4001 |
| A | HOH4002 |
| A | HOH4176 |
| A | HOH4190 |
| A | HOH4241 |
| A | HOH4315 |
| A | MG5001 |
| A | MG5002 |
| site_id | AC6 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE GGA B 6002 |
| Chain | Residue |
| B | ARG316 |
| B | ASP318 |
| B | GLU330 |
| B | ASN332 |
| B | SER335 |
| B | ALA336 |
| B | SER337 |
| B | GLU341 |
| B | ASP387 |
| B | GLU391 |
| B | GLU392 |
| B | THR441 |
| B | TRP442 |
| B | ALA443 |
| B | THR446 |
| B | ARG538 |
| B | CYS539 |
| B | ARG598 |
| B | LYS607 |
| B | SER609 |
| B | ASP610 |
| B | ADP3002 |
| B | HOH4029 |
| B | HOH4467 |
| B | MG5003 |
| B | MG5004 |
| site_id | AC7 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE ADP A 3001 |
| Chain | Residue |
| A | ASP318 |
| A | TYR329 |
| A | GLU330 |
| A | LYS498 |
| A | ALA531 |
| A | LYS533 |
| A | ARG538 |
| A | CYS539 |
| A | GLY540 |
| A | ILE543 |
| A | GLN568 |
| A | GLN569 |
| A | LEU570 |
| A | TRP571 |
| A | LEU573 |
| A | GLN582 |
| A | LEU603 |
| A | ILE605 |
| A | HOH4018 |
| A | HOH4190 |
| A | MG5001 |
| A | MG5002 |
| A | GGA6001 |
| site_id | AC8 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE ADP B 3002 |
| Chain | Residue |
| B | GLU330 |
| B | LYS498 |
| B | ALA531 |
| B | LYS533 |
| B | ARG538 |
| B | CYS539 |
| B | GLY540 |
| B | GLN568 |
| B | GLN569 |
| B | TRP571 |
| B | GLN582 |
| B | LEU603 |
| B | VAL604 |
| B | ILE605 |
| B | HOH4293 |
| B | MG5003 |
| B | MG5004 |
| B | GGA6002 |
| B | ASP318 |
| B | TYR329 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate => ECO:0000269|PubMed:9398217 |
| Chain | Residue | Details |
| A | CYS59 | |
| B | CYS59 | |
| site_id | SWS_FT_FI2 |
| Number of Residues | 38 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | VAL78 | |
| A | ASN149 | |
| A | ARG316 | |
| A | ASP318 | |
| A | GLU330 | |
| A | ASN332 | |
| A | SER335 | |
| A | GLU391 | |
| A | GLU392 | |
| A | THR446 | |
| A | LYS498 | |
| A | LYS533 | |
| A | CYS539 | |
| A | GLN568 | |
| A | GLN582 | |
| A | LEU603 | |
| A | ASP610 | |
| B | GLN58 | |
| B | ARG64 | |
| B | VAL78 | |
| B | ASN149 | |
| B | ARG316 | |
| B | ASP318 | |
| B | GLU330 | |
| B | ASN332 | |
| B | SER335 | |
| B | GLU391 | |
| B | GLU392 | |
| B | THR446 | |
| B | LYS498 | |
| B | LYS533 | |
| B | CYS539 | |
| B | GLN568 | |
| B | GLN582 | |
| B | LEU603 | |
| B | ASP610 | |
| A | GLN58 | |
| A | ARG64 | |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Increases nucleophilicity of active site Cys; for amidase activity |
| Chain | Residue | Details |
| A | HIS131 | |
| B | HIS131 | |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | SITE: Transition state stabilizer; for synthetase activity |
| Chain | Residue | Details |
| A | ARG316 | |
| B | ARG316 | |
| Chain | Residue | Details |
| A | CYS59 | |
| B | CYS59 | |
