2IO7
E. coli Bifunctional glutathionylspermidine synthetase/amidase Incomplex with Mg2+ and AMPPNP
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0005515 | molecular_function | protein binding |
A | 0005524 | molecular_function | ATP binding |
A | 0005829 | cellular_component | cytosol |
A | 0006749 | biological_process | glutathione metabolic process |
A | 0008216 | biological_process | spermidine metabolic process |
A | 0008884 | molecular_function | glutathionylspermidine amidase activity |
A | 0008885 | molecular_function | glutathionylspermidine synthase activity |
A | 0016787 | molecular_function | hydrolase activity |
A | 0016874 | molecular_function | ligase activity |
A | 0046872 | molecular_function | metal ion binding |
B | 0005515 | molecular_function | protein binding |
B | 0005524 | molecular_function | ATP binding |
B | 0005829 | cellular_component | cytosol |
B | 0006749 | biological_process | glutathione metabolic process |
B | 0008216 | biological_process | spermidine metabolic process |
B | 0008884 | molecular_function | glutathionylspermidine amidase activity |
B | 0008885 | molecular_function | glutathionylspermidine synthase activity |
B | 0016787 | molecular_function | hydrolase activity |
B | 0016874 | molecular_function | ligase activity |
B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE MG A 5001 |
Chain | Residue |
A | GLU330 |
A | ASN332 |
A | GLY537 |
A | ANP964 |
site_id | AC2 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE MG A 5002 |
Chain | Residue |
A | ASP318 |
A | GLU330 |
A | ANP964 |
site_id | AC3 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE MG B 5003 |
Chain | Residue |
B | ANP965 |
B | GLU330 |
B | ASN332 |
site_id | AC4 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE MG B 5004 |
Chain | Residue |
B | ASP318 |
B | GLU330 |
B | ANP965 |
site_id | AC5 |
Number of Residues | 25 |
Details | BINDING SITE FOR RESIDUE ANP A 964 |
Chain | Residue |
A | ARG316 |
A | ASP318 |
A | TYR329 |
A | GLU330 |
A | ASN332 |
A | LYS498 |
A | ALA531 |
A | LYS533 |
A | GLY537 |
A | ARG538 |
A | CYS539 |
A | GLY540 |
A | GLN568 |
A | GLN569 |
A | LEU570 |
A | TRP571 |
A | LEU573 |
A | GLN582 |
A | LEU603 |
A | VAL604 |
A | ILE605 |
A | MG5001 |
A | MG5002 |
A | HOH5026 |
A | HOH5036 |
site_id | AC6 |
Number of Residues | 24 |
Details | BINDING SITE FOR RESIDUE ANP B 965 |
Chain | Residue |
B | ARG316 |
B | ASP318 |
B | TYR329 |
B | GLU330 |
B | ASN332 |
B | LYS498 |
B | ALA531 |
B | LYS533 |
B | GLY537 |
B | ARG538 |
B | CYS539 |
B | GLY540 |
B | GLN568 |
B | GLN569 |
B | LEU570 |
B | TRP571 |
B | LEU573 |
B | GLN582 |
B | LEU603 |
B | VAL604 |
B | ILE605 |
B | MG5003 |
B | MG5004 |
B | HOH5199 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate => ECO:0000269|PubMed:9398217 |
Chain | Residue | Details |
A | CYS59 | |
B | CYS59 |
site_id | SWS_FT_FI2 |
Number of Residues | 38 |
Details | BINDING: |
Chain | Residue | Details |
A | VAL78 | |
A | ASN149 | |
A | ARG316 | |
A | ASP318 | |
A | GLU330 | |
A | ASN332 | |
A | SER335 | |
A | GLU391 | |
A | GLU392 | |
A | THR446 | |
A | LYS498 | |
A | LYS533 | |
A | CYS539 | |
A | GLN568 | |
A | GLN582 | |
A | LEU603 | |
A | ASP610 | |
B | GLN58 | |
B | ARG64 | |
B | VAL78 | |
B | ASN149 | |
B | ARG316 | |
B | ASP318 | |
B | GLU330 | |
B | ASN332 | |
B | SER335 | |
B | GLU391 | |
B | GLU392 | |
B | THR446 | |
B | LYS498 | |
B | LYS533 | |
B | CYS539 | |
B | GLN568 | |
B | GLN582 | |
B | LEU603 | |
B | ASP610 | |
A | GLN58 | |
A | ARG64 |
site_id | SWS_FT_FI3 |
Number of Residues | 2 |
Details | SITE: Increases nucleophilicity of active site Cys; for amidase activity |
Chain | Residue | Details |
A | HIS131 | |
B | HIS131 |
site_id | SWS_FT_FI4 |
Number of Residues | 2 |
Details | SITE: Transition state stabilizer; for synthetase activity |
Chain | Residue | Details |
A | ARG316 | |
B | ARG316 |
site_id | SWS_FT_FI5 |
Number of Residues | 2 |
Details | MOD_RES: Cysteine sulfenic acid (-SOH); transient => ECO:0000269|PubMed:20530482 |
Chain | Residue | Details |
A | CYS59 | |
B | CYS59 |