2IK4

Yeast inorganic pyrophosphatase variant D117E with magnesium and phosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004427	molecular_function	inorganic diphosphate phosphatase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0006796	biological_process	phosphate-containing compound metabolic process
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004427	molecular_function	inorganic diphosphate phosphatase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0006796	biological_process	phosphate-containing compound metabolic process
B	0016787	molecular_function	hydrolase activity
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 287

Chain	Residue
A	ASP115
A	ASP120
A	ASP152
A	HOH289
A	HOH293
A	HOH294

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 288

Chain	Residue
A	HOH292
A	HOH295
A	HOH296
A	ASP120
A	HOH290
A	HOH291

---

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MG B 287

Chain	Residue
B	ASP115
B	GLU117
B	ASP120
B	ASP152
B	MG288
B	MG289
B	PO4290
B	HOH291

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 288

Chain	Residue
B	GLU117
B	ASP120
B	MG287
B	PO4290
B	HOH292
B	HOH293
B	HOH294

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 289

Chain	Residue
B	ASP147
B	ASP152
B	MG287
B	PO4290
B	HOH295
B	HOH296
B	HOH297

---

site_id	AC6
Number of Residues	17
Details	BINDING SITE FOR RESIDUE PO4 B 290

Chain	Residue
B	LYS56
B	GLU58
B	TYR93
B	ASP115
B	GLU117
B	ASP120
B	ASP152
B	LYS154
B	MG287
B	MG288
B	MG289
B	HOH294
B	HOH295
B	HOH296
B	HOH297
B	HOH376
B	HOH427

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"1322842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"15665377","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1wgi

Chain	Residue	Details
A	GLU117

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1wgi

Chain	Residue	Details
B	GLU117

---