Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2I2X

Crystal structure of methanol:cobalamin methyltransferase complex MtaBC from Methanosarcina barkeri

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0008168	molecular_function	methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
A	0046872	molecular_function	metal ion binding
A	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0008168	molecular_function	methyltransferase activity
B	0008705	molecular_function	methionine synthase activity
B	0009086	biological_process	methionine biosynthetic process
B	0015948	biological_process	methanogenesis
B	0031419	molecular_function	cobalamin binding
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046872	molecular_function	metal ion binding
B	0050667	biological_process	homocysteine metabolic process
B	0050897	molecular_function	cobalt ion binding
B	0071267	biological_process	L-methionine salvage
C	0005515	molecular_function	protein binding
C	0008168	molecular_function	methyltransferase activity
C	0016740	molecular_function	transferase activity
C	0032259	biological_process	methylation
C	0046872	molecular_function	metal ion binding
C	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0008168	molecular_function	methyltransferase activity
D	0008705	molecular_function	methionine synthase activity
D	0009086	biological_process	methionine biosynthetic process
D	0015948	biological_process	methanogenesis
D	0031419	molecular_function	cobalamin binding
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0046872	molecular_function	metal ion binding
D	0050667	biological_process	homocysteine metabolic process
D	0050897	molecular_function	cobalt ion binding
D	0071267	biological_process	L-methionine salvage
E	0005515	molecular_function	protein binding
E	0008168	molecular_function	methyltransferase activity
E	0016740	molecular_function	transferase activity
E	0032259	biological_process	methylation
E	0046872	molecular_function	metal ion binding
E	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0008168	molecular_function	methyltransferase activity
F	0008705	molecular_function	methionine synthase activity
F	0009086	biological_process	methionine biosynthetic process
F	0015948	biological_process	methanogenesis
F	0031419	molecular_function	cobalamin binding
F	0046653	biological_process	tetrahydrofolate metabolic process
F	0046872	molecular_function	metal ion binding
F	0050667	biological_process	homocysteine metabolic process
F	0050897	molecular_function	cobalt ion binding
F	0071267	biological_process	L-methionine salvage
G	0005515	molecular_function	protein binding
G	0008168	molecular_function	methyltransferase activity
G	0016740	molecular_function	transferase activity
G	0032259	biological_process	methylation
G	0046872	molecular_function	metal ion binding
G	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0008168	molecular_function	methyltransferase activity
H	0008705	molecular_function	methionine synthase activity
H	0009086	biological_process	methionine biosynthetic process
H	0015948	biological_process	methanogenesis
H	0031419	molecular_function	cobalamin binding
H	0046653	biological_process	tetrahydrofolate metabolic process
H	0046872	molecular_function	metal ion binding
H	0050667	biological_process	homocysteine metabolic process
H	0050897	molecular_function	cobalt ion binding
H	0071267	biological_process	L-methionine salvage
I	0005515	molecular_function	protein binding
I	0008168	molecular_function	methyltransferase activity
I	0016740	molecular_function	transferase activity
I	0032259	biological_process	methylation
I	0046872	molecular_function	metal ion binding
I	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
J	0005515	molecular_function	protein binding
J	0005829	cellular_component	cytosol
J	0008168	molecular_function	methyltransferase activity
J	0008705	molecular_function	methionine synthase activity
J	0009086	biological_process	methionine biosynthetic process
J	0015948	biological_process	methanogenesis
J	0031419	molecular_function	cobalamin binding
J	0046653	biological_process	tetrahydrofolate metabolic process
J	0046872	molecular_function	metal ion binding
J	0050667	biological_process	homocysteine metabolic process
J	0050897	molecular_function	cobalt ion binding
J	0071267	biological_process	L-methionine salvage
K	0005515	molecular_function	protein binding
K	0008168	molecular_function	methyltransferase activity
K	0016740	molecular_function	transferase activity
K	0032259	biological_process	methylation
K	0046872	molecular_function	metal ion binding
K	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
L	0005515	molecular_function	protein binding
L	0005829	cellular_component	cytosol
L	0008168	molecular_function	methyltransferase activity
L	0008705	molecular_function	methionine synthase activity
L	0009086	biological_process	methionine biosynthetic process
L	0015948	biological_process	methanogenesis
L	0031419	molecular_function	cobalamin binding
L	0046653	biological_process	tetrahydrofolate metabolic process
L	0046872	molecular_function	metal ion binding
L	0050667	biological_process	homocysteine metabolic process
L	0050897	molecular_function	cobalt ion binding
L	0071267	biological_process	L-methionine salvage
M	0005515	molecular_function	protein binding
M	0008168	molecular_function	methyltransferase activity
M	0016740	molecular_function	transferase activity
M	0032259	biological_process	methylation
M	0046872	molecular_function	metal ion binding
M	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
N	0005515	molecular_function	protein binding
N	0005829	cellular_component	cytosol
N	0008168	molecular_function	methyltransferase activity
N	0008705	molecular_function	methionine synthase activity
N	0009086	biological_process	methionine biosynthetic process
N	0015948	biological_process	methanogenesis
N	0031419	molecular_function	cobalamin binding
N	0046653	biological_process	tetrahydrofolate metabolic process
N	0046872	molecular_function	metal ion binding
N	0050667	biological_process	homocysteine metabolic process
N	0050897	molecular_function	cobalt ion binding
N	0071267	biological_process	L-methionine salvage
O	0005515	molecular_function	protein binding
O	0008168	molecular_function	methyltransferase activity
O	0016740	molecular_function	transferase activity
O	0032259	biological_process	methylation
O	0046872	molecular_function	metal ion binding
O	0047152	molecular_function	methanol-5-hydroxybenzimidazolylcobamide Co-methyltransferase activity
P	0005515	molecular_function	protein binding
P	0005829	cellular_component	cytosol
P	0008168	molecular_function	methyltransferase activity
P	0008705	molecular_function	methionine synthase activity
P	0009086	biological_process	methionine biosynthetic process
P	0015948	biological_process	methanogenesis
P	0031419	molecular_function	cobalamin binding
P	0046653	biological_process	tetrahydrofolate metabolic process
P	0046872	molecular_function	metal ion binding
P	0050667	biological_process	homocysteine metabolic process
P	0050897	molecular_function	cobalt ion binding
P	0071267	biological_process	L-methionine salvage

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 501

Chain	Residue
A	GLU164
A	CYS220
A	CYS269
A	K511

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 524

Chain	Residue
A	HIS318
A	GLU320
C	HIS318
C	GLU320

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K A 511

Chain	Residue
A	GLU164
A	GLU313
A	ZN501
A	GLU86

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 502

Chain	Residue
C	GLU164
C	CYS220
C	CYS269
C	K512

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE K C 512

Chain	Residue
C	GLU86
C	GLU164
C	GLU313
C	ZN502
C	HOH679

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 503

Chain	Residue
E	GLU164
E	CYS220
E	CYS269
E	K513

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 522

Chain	Residue
E	HIS318
E	GLU320
G	HIS318
G	GLU320

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K E 513

Chain	Residue
E	GLU86
E	GLU164
E	GLU313
E	ZN503

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN G 504

Chain	Residue
G	GLU164
G	CYS220
G	CYS269
G	GLU313
G	K514

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K G 514

Chain	Residue
G	GLU86
G	GLU164
G	GLU313
G	ZN504

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN I 505

Chain	Residue
I	GLU164
I	CYS220
I	CYS269
I	K515

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN I 521

Chain	Residue
I	HIS318
I	GLU320
K	HIS318
K	GLU320

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K I 515

Chain	Residue
I	GLU86
I	GLU164
I	GLU313
I	ZN505

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN K 506

Chain	Residue
K	GLU164
K	CYS220
K	CYS269
K	K516

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K K 516

Chain	Residue
K	GLU86
K	GLU164
K	GLU313
K	ZN506

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN M 507

Chain	Residue
M	GLU164
M	CYS220
M	CYS269
M	K517

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN M 523

Chain	Residue
M	HIS318
M	GLU320
O	HIS318
O	GLU320

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE K M 517

Chain	Residue
M	GLU86
M	GLU164
M	GLU313
M	ZN507
M	HOH615

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN O 508

Chain	Residue
O	GLU164
O	CYS220
O	CYS269
O	K518

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K O 518

Chain	Residue
O	GLU86
O	GLU164
O	GLU313
O	ZN508

site_id	CC3
Number of Residues	32
Details	BINDING SITE FOR RESIDUE B13 B 500

Chain	Residue
B	THR181
B	LEU183
B	MET184
B	THR185
B	ALA208
B	GLY210
B	GLY211
B	GLY212
B	GLY228
B	GLU229
B	GLU230
B	ALA231
B	HOH504
B	HOH512
B	HOH534
C	PHE321
A	LYS169
A	ASP173
A	THR225
A	PHE228
A	ALA294
A	HOH606
B	GLY133
B	ASP134
B	VAL135
B	HIS136
B	ASP137
B	ILE138
B	GLY139
B	VAL143
B	THR179
B	GLY180

site_id	CC4
Number of Residues	33
Details	BINDING SITE FOR RESIDUE B13 D 500

Chain	Residue
A	PHE321
C	LYS169
C	ASP173
C	THR225
C	PHE228
C	ALA294
C	SER314
C	HOH643
D	GLY133
D	ASP134
D	VAL135
D	HIS136
D	ASP137
D	ILE138
D	GLY139
D	VAL143
D	THR179
D	GLY180
D	THR181
D	LEU183
D	MET184
D	THR185
D	ALA208
D	GLY210
D	GLY211
D	GLY212
D	GLY228
D	GLU229
D	GLU230
D	ALA231
D	HOH507
D	HOH512
D	HOH527

site_id	CC5
Number of Residues	29
Details	BINDING SITE FOR RESIDUE B13 F 500

Chain	Residue
E	LYS169
E	ASP173
E	THR225
E	PHE228
E	ALA294
E	SER314
F	GLY133
F	VAL135
F	HIS136
F	ASP137
F	ILE138
F	GLY139
F	VAL143
F	THR179
F	GLY180
F	THR181
F	LEU183
F	MET184
F	THR185
F	ALA208
F	CYS209
F	GLY210
F	GLY211
F	GLY212
F	GLY228
F	GLU229
F	GLU230
F	ALA231
G	PHE321

site_id	CC6
Number of Residues	29
Details	BINDING SITE FOR RESIDUE B13 H 500

Chain	Residue
E	PHE321
G	LYS169
G	ASP173
G	THR225
G	PHE228
G	ALA294
G	SER314
H	GLY133
H	ASP134
H	VAL135
H	HIS136
H	ASP137
H	ILE138
H	GLY139
H	VAL143
H	THR179
H	GLY180
H	THR181
H	LEU183
H	MET184
H	THR185
H	ALA208
H	GLY210
H	GLY211
H	GLY212
H	GLY228
H	GLU229
H	GLU230
H	ALA231

site_id	CC7
Number of Residues	28
Details	BINDING SITE FOR RESIDUE B13 J 500

Chain	Residue
I	LYS169
I	THR225
I	PHE228
I	ALA294
I	SER314
J	GLY133
J	ASP134
J	VAL135
J	HIS136
J	ASP137
J	ILE138
J	GLY139
J	ILE142
J	THR179
J	GLY180
J	THR181
J	LEU183
J	MET184
J	THR185
J	ALA208
J	GLY210
J	GLY211
J	GLY212
J	GLY228
J	GLU229
J	GLU230
J	ALA231
K	PHE321

site_id	CC8
Number of Residues	33
Details	BINDING SITE FOR RESIDUE B13 L 500

Chain	Residue
I	PHE321
K	LYS169
K	ASP173
K	ALA221
K	THR225
K	PHE228
K	ALA294
L	GLY133
L	ASP134
L	VAL135
L	HIS136
L	ASP137
L	ILE138
L	GLY139
L	VAL143
L	THR179
L	GLY180
L	THR181
L	LEU183
L	MET184
L	THR185
L	ALA208
L	GLY210
L	GLY211
L	GLY212
L	GLY228
L	GLU229
L	GLU230
L	ALA231
L	HOH501
L	HOH505
L	HOH507
L	HOH514

site_id	CC9
Number of Residues	27
Details	BINDING SITE FOR RESIDUE B13 N 500

Chain	Residue
M	LYS169
M	THR225
M	ALA294
M	SER314
N	GLY133
N	VAL135
N	HIS136
N	ASP137
N	ILE138
N	GLY139
N	VAL143
N	THR179
N	GLY180
N	THR181
N	LEU183
N	MET184
N	THR185
N	ALA208
N	GLY210
N	GLY211
N	GLY212
N	GLY228
N	GLU229
N	GLU230
N	ALA231
N	HOH518
O	PHE321

site_id	DC1
Number of Residues	28
Details	BINDING SITE FOR RESIDUE B13 P 500

Chain	Residue
M	PHE321
O	LYS169
O	ASP173
O	THR225
O	PHE228
O	ALA294
O	SER314
O	HOH592
P	GLY133
P	VAL135
P	HIS136
P	ASP137
P	ILE138
P	GLY139
P	VAL143
P	THR179
P	THR181
P	LEU183
P	MET184
P	THR185
P	ALA208
P	GLY210
P	GLY211
P	GLY212
P	GLY228
P	GLU229
P	GLU230
P	ALA231

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17142327","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	752
Details	Domain: {"description":"B12-binding N-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00667","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	1000
Details	Domain: {"description":"B12-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00666","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
B	THR187
B	HIS136
B	ASP134

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
D	THR187
D	HIS136
D	ASP134

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
F	THR187
F	HIS136
F	ASP134

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
H	THR187
H	HIS136
H	ASP134

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
J	THR187
J	HIS136
J	ASP134

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
L	THR187
L	HIS136
L	ASP134

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
N	THR187
N	HIS136
N	ASP134

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
P	THR187
P	HIS136
P	ASP134

site_id	CSA9
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
B	LEU147

site_id	CSA10
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
D	LEU147

site_id	CSA11
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
F	LEU147

site_id	CSA12
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
H	LEU147

site_id	CSA13
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
J	LEU147

site_id	CSA14
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
L	LEU147

site_id	CSA15
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
N	LEU147

site_id	CSA16
Number of Residues	1
Details	Annotated By Reference To The Literature 1bmt

Chain	Residue	Details
P	LEU147

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)