2HIS

CELLULOMONAS FIMI XYLANASE/CELLULASE DOUBLE MUTANT E127A/H205N WITH COVALENT CELLOBIOSE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process

Functional Information from PDB Data

site_id	NUC
Number of Residues	1
Details	CATALYTIC NUCLEOPHILE, COVALENTLY LINKED TO CELLOBIOSE.

Chain	Residue
A	GLU233

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Functional Information from PROSITE/UniProt

site_id	PS00591
Number of Residues	11
Details	GH10_1 Glycosyl hydrolases family 10 (GH10) active site. GVDVrITELDI

Chain	Residue	Details
A	GLY226-ILE236

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	310
Details	Domain: {"description":"GH10","evidences":[{"source":"PROSITE-ProRule","id":"PRU01096","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"7918478","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10061","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1678739","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	a catalytic site defined by CSA, PubMed 1678739, 9731776, 7910761, 7901761

Chain	Residue	Details
A	ALA127

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 548

Chain	Residue	Details
A	ALA127	proton acceptor, proton donor
A	ASN169	electrostatic stabiliser
A	ASN205	electrostatic stabiliser
A	GLU233	electrostatic stabiliser, nucleofuge, nucleophile
A	ASP235	electrostatic stabiliser

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