2HFJ
Pikromycin thioesterase with covalent pentaketide affinity label
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 501 |
| Chain | Residue |
| A | ARG24 |
| B | ALA37 |
| B | GLN41 |
| B | ARG215 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 502 |
| Chain | Residue |
| A | ALA37 |
| A | GLN41 |
| A | ARG215 |
| B | ARG24 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 504 |
| Chain | Residue |
| B | GLU246 |
| B | GLU247 |
| A | ARG248 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 509 |
| Chain | Residue |
| A | HIS254 |
| A | ASP256 |
| A | ASP256 |
| A | HOH561 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS B 503 |
| Chain | Residue |
| B | PRO179 |
| B | ARG227 |
| B | HIS254 |
| B | TRP255 |
| B | ASP256 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 2036 |
| Chain | Residue |
| A | GLN192 |
| A | ASP243 |
| A | HOH514 |
| B | ARG191 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE YML A 448 |
| Chain | Residue |
| A | TYR25 |
| A | LEU29 |
| A | THR77 |
| A | ALA78 |
| A | SER148 |
| A | GLY149 |
| A | MET213 |
| A | HIS268 |
| A | HOH541 |
| A | HOH586 |
| A | HOH685 |
| A | HOH698 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile; for thioesterase activity => ECO:0000250|UniProtKB:Q03133, ECO:0000269|PubMed:16969372, ECO:0000305|PubMed:16969373 |
| Chain | Residue | Details |
| A | SER148 | |
| B | SER148 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor; for thioesterase activity => ECO:0000250|UniProtKB:Q03133, ECO:0000305|PubMed:16969372, ECO:0000305|PubMed:16969373 |
| Chain | Residue | Details |
| A | HIS268 | |
| B | HIS268 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:16969372 |
| Chain | Residue | Details |
| A | THR77 | |
| B | THR77 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:16969372, ECO:0000269|PubMed:16969373 |
| Chain | Residue | Details |
| A | GLY149 | |
| B | GLY149 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q03133 |
| Chain | Residue | Details |
| A | ASP176 | |
| B | ASP176 |
