2H5C

0.82A resolution crystal structure of alpha-lytic protease at pH 5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006508	biological_process	proteolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SO4 A 246

Chain	Residue
A	ARG48
A	HOH559
A	HOH568
A	HOH596
A	HOH635
A	HOH698
A	HOH707
A	HOH718
A	HOH719
A	ARG120
A	GLN237
A	TYR238
A	HOH389
A	HOH390
A	HOH391
A	HOH485
A	HOH534

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE SO4 A 247

Chain	Residue
A	ALA15
A	ASN15
A	ARG230
A	PRO233
A	HOH333
A	HOH403
A	HOH434
A	HOH460
A	HOH488
A	HOH509
A	HOH633
A	HOH634
A	HOH643

---

site_id	AC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE SO4 A 248

Chain	Residue
A	HIS57
A	ARG122
A	ARG192
A	GLY193
A	SER195
A	GOL253
A	HOH424
A	HOH459
A	HOH491
A	HOH668
A	HOH669
A	HOH670
A	HOH671
A	HOH676

---

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 203

Chain	Residue
A	THR59
A	VAL59
A	ARG90
A	HOH386
A	HOH393
A	HOH468
A	HOH562
A	HOH626
A	HOH627
A	HOH708

---

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE SO4 A 204

Chain	Residue
A	ALA48
A	THR49
A	SER110
A	ALA176
A	ARG178
A	HOH309
A	HOH349
A	HOH461
A	HOH555
A	HOH573
A	HOH661
A	HOH662
A	HOH730

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 205

Chain	Residue
A	THR168
A	ARG178
A	HOH417
A	HOH549
A	HOH709
A	HOH731

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 206

Chain	Residue
A	GLN219
A	SER219
A	HOH353
A	HOH355
A	HOH630
A	HOH700

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 249

Chain	Residue
A	ASN36
A	THR62
A	ARG65
A	HOH365
A	HOH599
A	HOH701
A	HOH702

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 250

Chain	Residue
A	ALA131
A	VAL132
A	LYS165
A	HOH566
A	HOH646
A	HOH703
A	HOH711

---

site_id	BC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE SO4 A 251

Chain	Residue
A	HOH529
A	HOH548
A	HOH567
A	HOH590
A	HOH611
A	HOH623
A	HOH665
A	HOH674
A	HOH712
A	ARG141
A	HOH314
A	HOH380
A	HOH432
A	HOH527
A	HOH528

---

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 252

Chain	Residue
A	ASN217
A	GLN219
A	HOH569
A	HOH679
A	HOH720
A	HOH721
A	HOH722

---

site_id	BC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE GOL A 253

Chain	Residue
A	HIS57
A	ARG122
A	TYR171
A	GLU174
A	ARG192
A	SER214
A	GLY215
A	SO4248
A	HOH337
A	HOH474
A	HOH477
A	HOH491
A	HOH629
A	HOH671
A	HOH745

---

site_id	BC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 254

Chain	Residue
A	PHE120
A	VAL120
A	THR120
A	ALA173
A	GLU174
A	HOH489
A	HOH531
A	HOH532
A	HOH576

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VTAGHC

Chain	Residue	Details
A	VAL53-CYS58

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. CMgrGDSGGSWI

Chain	Residue	Details
A	CYS189-ILE200

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Active site: {"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	5
Details	Annotated By Reference To The Literature 1ssx

Chain	Residue	Details
A	ASP102
A	SER195
A	GLY193
A	HIS57
A	SER214

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ssx

Chain	Residue	Details
A	ASP102
A	SER195
A	GLY193
A	HIS57

---

site_id	MCSA1
Number of Residues	5
Details	M-CSA 609

Chain	Residue	Details
A	HIS57	proton acceptor, proton donor
A	ASP102	electrostatic stabiliser
A	GLY193	electrostatic stabiliser
A	SER195	electrostatic stabiliser
A	SER214	electrostatic stabiliser

---